Next-generation biosensors for detecting bioaerosols: Challenges, innovations, and applications [0.03%]
Krishan Kumar
Krishan Kumar
Bioaerosols, which include airborne bacteria, viruses, fungi, spores, and biological toxins, are an important but understudied component of atmospheric particulate matter, with serious consequences for public health, environmental safety, a...
Methodological validation of microbial limit tests for high-viscosity and common pharmaceutical excipients [0.03%]
Xi Wang,Yuying Zhao,Jing Wang et al.
Xi Wang et al.
Microbial contamination of pharmaceutical excipients remains a critical precursor to finished product failure, which necessitates rigorous microbial limit testing of pharmaceutical excipients. However, high-viscosity excipients present dist...
Innovation in acid-fast staining methods: A comparative study of a modified staining rack, a conventional rack and an automated stainer [0.03%]
Liping Wang,Lijun Kong,Pei Zhang et al.
Liping Wang et al.
Manual acid-fast staining often results in specimen slippage, inaccurate positioning, and increased biosafety risks. This study evaluated a modified staining rack incorporating a slide-positioning device and an adjustable tilt function to i...
Response surface methodology optimization of cell-free supernatant from P. pentosaceus BJQ fermentation of CeRS3 and its in vitro lipid-lowering effects [0.03%]
Maochun Xiao,Aji Li,Chunying Song et al.
Maochun Xiao et al.
This study established and optimized a high-density fermentation process for BJQ-CeRS3, a co-fermentation system of Pediococcus pentosaceus BJQ (P. pentosaceus BJQ) and type 3 resistant starch derived from canna (CeRS3), and evaluated its m...
Effects of 16S rRNA hypervariable region selection on respiratory microbiome profiling in healthy adults [0.03%]
Lijun Liang,Yuqi Fu,Dawei Guan et al.
Lijun Liang et al.
Selection of the 16S rRNA gene target region can substantially influence inferred respiratory microbiome profiles, particularly in low-biomass samples. In this study, we compared full-length V1-V9 (Pacific Biosciences) and V3-V4 (Illumina) ...
Molecular detection of foodborne pathogens with emphasis on multiplex-allele-specific PCR, computational primer design, and gene amplification approaches [0.03%]
Rajkumar Prabhakaran,Meenakshi Sundari Rajendran,Ramkumar Muthu
Rajkumar Prabhakaran
Foodborne diseases continue to be major public health challenges around the world, with contaminated food linked to an estimated 420,000 deaths annually, many of which are children under five. Traditional testing methods for foodborne patho...
Biomolecular insights into opportunistic pathogens and a risk-threshold control strategy in rural household hot water systems [0.03%]
Hai Wang,Kangyin Guo,Jingang Huang et al.
Hai Wang et al.
Opportunistic pathogens (OPs) are typical biological indicators for public health monitoring in both freshwater and freshwater-derived engineered waters. This study investigated the OPs abundance and microbial communities in household hot w...
Establishment of autoclave conditions for disaccharide-containing liquid media using spectrophotometry and high-performance liquid chromatography [0.03%]
Kozue Shimabukuro,Taito Kobayashi,Eiji Yokoyama et al.
Kozue Shimabukuro et al.
Disaccharides undergo heat-induced hydrolysis, generating monosaccharides. In Japan, 10% sucrose solution autoclaved at 121 °C for 10 min is commonly added to basal media, but this caused false-positive fermentation for Shigella spp. Spect...
Advancing viral detection: Recent progress in phage immunoprecipitation sequencing (PhIP-Seq) for immune profiling and surveillance [0.03%]
Gustavo Carvalho Amorim,Hernan Hermes Monteiro da Costa,Carlos Henrique Gomes et al.
Gustavo Carvalho Amorim et al.
Global efforts to characterize emerging or reemerging viruses from wildlife strongly depend on comprehensive detection platforms based on highly multiplexed serology. With the rising risk of new pandemics, there is an increasing need for im...
Redefining Xylella fastidiosa cultivation: An improved growth system for faster physiological and antimicrobial studies [0.03%]
Eduardo Costa-Camilo,Tomás Martins,Cláudia Carvalho et al.
Eduardo Costa-Camilo et al.
Xylella fastidiosa is a xylem-limited, Gram-negative phytopathogenic bacterium responsible for severe diseases affecting a wide range of economically important crops worldwide. Its recent expansion into Europe has reinforced its status as a...