A rapid and visual detection for canine Adenovirus-2 using CRISPR-Cas13a-based SHERLOCK technology [0.03%]
基于CRISPR-Cas13a的SHERLOCK技术在犬腺病毒-2快速可视化检测中的应用研究
Boyu Liu,Yifan Li,Zaixing Yang et al.
Boyu Liu et al.
Canine adenovirus type 2 (CAdV-2) is an important pathogen causing infectious tracheobronchitis (ITB) and viral enteritis in puppies, often exacerbating clinical symptoms through co-infection with other viruses. However, existing diagnostic...
Comparison of three commercial DNA extraction kits and assemblers for AMR determinant detection in Pseudomonas aeruginosa and Enterobacter cloacae using long-read sequencing [0.03%]
基于长读测序的铜绿假单胞菌和阴沟肠杆菌AMR决定因子检测的三种商业DNA提取试剂盒和组装工具比较
Anurag Kumar Bari,Basil Britto Xavier,Tim Severs et al.
Anurag Kumar Bari et al.
Background: Pseudomonas aeruginosa and Enterobacter cloacae, both members of the ESKAPE group, are multidrug-resistant pathogens that pose significant challenges in clinical care. The high GC-content (∼67 %) of the P. ae...
A workflow for selective isolation of bacterial RNA from Streptococcus agalactiae during in vivo infection [0.03%]
小鼠感染期间选择性分离链球菌RNA的工作流程
Agata Famà,Germana Lentini,Alessia Berbiglia et al.
Agata Famà et al.
Studying bacterial gene expression directly in infected tissues is crucial for understanding host-pathogen interactions, however recovery of intact bacterial RNA in vivo remains technically challenging. Contamination with host RNA and the i...
Comparison of diagnostic efficacy of three different oral samples in pulmonary tuberculosis using cepheid gene Xpert® MTB/RIF ultra [0.03%]
比较三种不同口腔样本使用Cepheid Gene Xpert® MTB/RIF Ultra检测肺结核诊断效能的研究
Yan Liu,Qianfang Hu,Shuliang Guo
Yan Liu
Context: The etiological confirmation of pulmonary tuberculosis typically relies on sputum or bronchoalveolar lavage fluid (BALF). Oral sampling offers a non-invasive and non-sputum alternative, but evidence varies widely...
Development of a bi-epitope peptide as fail-safe internal control for a modified proximity extension assay and implementation in the detection of procalcitonin in human plasma [0.03%]
一种双表位肽的开发及其作为失效安全内部控制在人血浆中检测降钙素原中的应用：用于改进型邻近延伸测定法
Bedin Frederic,Benoit Vincent,Rubens Agnes et al.
Bedin Frederic et al.
Proximity Extension Assay (PEA) is a simple, minimally invasive assay developed for proteomic applications and biomarker detection in biological samples. The method is the combination of a homogeneous-phase immunoassay with quantitative PCR...
A rapid and efficient method for visualisation of microbial biofilms on natural, and industrially- and medically-relevant surfaces, using field emission- scanning electron microscopy [0.03%]
一种利用场发射扫描电子显微镜快速而高效地观察自然以及工业和医学相关表面微生物菌膜的方法
Abhinaba Chakraborty,Bomba Dam
Abhinaba Chakraborty
A rapid and efficient sample preparation method for visualising surface-associated microbial biofilms using Field Emission-Scanning Electron Microscopy (FE-SEM) was developed by optimising the fixative concentration and dehydration process....
Streamlined construction of episomal vectors for rapid assessment of fusion phytase display in Saccharomyces cerevisiae [0.03%]
用于快速评估融合植酸酶在酿酒酵母中展示的表型简化载体构建方法
Vo Thi Hoang Lan,Chau Quoc Cuong,Luc Mai Thanh et al.
Vo Thi Hoang Lan et al.
We developed a fusion phytase combining acid- and alkaline-active phytases, displayed on Saccharomyces cerevisiae cells. Recombinant replicative vectors were constructed to harbor phytase expression cassettes, enabling rapid screening of fu...
Kinetic analysis of dye reduction profiles provide deeper insights into the electron transfer activity of bacterial cultures [0.03%]
动力学分析染料还原谱图可更深入地洞察细菌培养物的电子转移活性
S K Shakthi Thangavel,Sahashransu S Mahapatra,Prajal Chettri et al.
S K Shakthi Thangavel et al.
The Dye Reduction-based Electron-transfer Activity Monitoring (DREAM) assay offers a rapid, scalable, and culture-independent method for assessing bacterial respiratory activity. In this study, we have developed and applied a kinetic framew...
"Evaluating the susceptibility of MBL carbapenemase-producing Enterobacteriaceae and Pseudomonas spp. to ceftazidime/avibactam plus aztreonam and Cefiderocol: A synergy study and susceptibility profile" [0.03%]
头孢他啶/阿维巴坦联合 azi 络氨卡青霉素和伊米配众对于金属酶碳青霉烯酶产生肠杆菌科细菌和铜绿假单胞菌的体外活性：一项协同作用研究及敏感性图谱分析
Ana Collazos Blanco,Ana Belén García Sáez,Carolina Plaza Cristobal et al.
Ana Collazos Blanco et al.
This study assessed the in vitro susceptibility of aztreonam/avibactam (ATM/AVI) and the synergy analyses against 38 clinical carbapenemase-producing strains (31 Enterobacterales and 7 Pseudomonas spp.) collected over two years. Susceptibil...
Deciphering microbial diversity and predicting metabolic functionalities in fermented pigmented rice water using culture-independent characterization [0.03%]
基于无培养特征解码发酵色素大米水的微生物多样性和预测其代谢功能
Shruti Mishra,Asif Ali Vadakkethil,Mir Asif Iquebal et al.
Shruti Mishra et al.
Fermented rice water is gaining importance lately due to its traditional food culture and potential beneficial effects. Flavored fermented rice water (FFRW) produced from pigmented rice varieties, viz., black, brown, and red, is shown to ha...