Determining the best method for assessing microbiological contamination on flexible endoscopes without a working channel: a pilot study [0.03%]
评估柔性无工作通道内镜微生物污染的最佳方法：一项试点研究
Yana Halmans,David J Wellenstein,Michael Romijn et al.
Yana Halmans et al.
Background: Flexible endoscopes (FEs) without a working channel can become extensively contaminated. Adequate reprocessing is critical and the quality of these processes can be verified by microbiological sampling. This s...
A rapid MALDI Biotyper based approach for screening anovaginal colonization by Streptococcus agalactiae in pregnant women [0.03%]
一种快速的基质辅助激光解吸电离生物型分析法用于筛查孕妇阴道中由产乳链球菌引起的感染
Suellen Bernardo de Queiroz,Carlos Gabriel Andrade Barbosa,Marcelo Henrique da Silva Pereira et al.
Suellen Bernardo de Queiroz et al.
Introduction: Colonization of pregnant women with Streptococcus agalactiae is the main risk factor for vertical transmission to neonates and the occurrence of early onset neonatal disease. Screening for maternal carriage ...
Unmasking metabolic endotoxemia: Proteinase K-enhanced detection of free and protein-associated endotoxin in human serum [0.03%]
利用蛋白酶K增强法检测人血清中的游离和蛋白结合脂多糖以揭示代谢内毒素血症真相
Alejandra Vargas-Caraveo,Keren E Tovar-Presas,Dalia S Flores-Molina et al.
Alejandra Vargas-Caraveo et al.
Metabolic endotoxemia is a low-grade endotoxin exposure that leaks from the gut into the bloodstream, contributing to low-grade chronic inflammation. The most used method to quantify endotoxins is the Limulus Amebocyte Lysate (LAL) assay; h...
Improving bacterial smear detection with Wright-Giemsa staining: A multicenter study in clinical microbiology laboratories [0.03%]
一种改良的wright-giemsa染色法可提高临床微生物室细菌涂片检出率：多中心研究
Pengyu Ji,Shuqiao Yuan,Tingting Zeng et al.
Pengyu Ji et al.
Improvement in bacterial detection in smear is needed because pathogens in smears and positive blood cultures (PBCs) can be missed by microscopy if Gram staining is used. This study assessed the efficacy of Wright-Giemsa staining in remedyi...
Rapid estimation of bacterial abundance in mouse feces by flow cytometry with SYBR green I and counting beads [0.03%]
SYBR Green I和计数微球的流式细胞仪技术在小鼠粪便细菌菌群快速定量中的应用研究
Virginia A Piqueras,Silvia G Correa
Virginia A Piqueras
Aims: To present a standardized and reproducible flow cytometry (FCM) protocol for the rapid estimation of bacterial abundance in mouse fecal samples using SYBR Green I and counting beads. ...
Microscale assay for the quantification of total polysaccharides to estimate extracellular polymeric substances (EPS) in soil [0.03%]
一种用于量化土壤中总多糖以估算胞外聚合物（EPS）的微尺度测定法
G Bogar,J T Lennon,H M Vander Stel et al.
G Bogar et al.
We present an inexpensive and practical microscale spectrophotometric acid-phenol assay to quantify polysaccharides in aqueous solution. The instant reaction within disposable materials is designed and benchmarked for use in quantifying ext...
Disk-based method for rapid determination of primary antituberculosis drug susceptibility in Mycobacterium tuberculosis [0.03%]
结核分枝杆菌一线抗结核药物敏感性盘基快速测定方法研究
Cemilenur Atas,Kubra Yildirim,Ahmet Yilmaz Coban
Cemilenur Atas
Rapid and accurate determination of Mycobacterium tuberculosis susceptibility to primary drugs is critical for the timely initiation of effective therapy and the control of resistant tuberculosis (TB). This study aims to evaluate disk-based...
Dioscorea tokoro Makino extract suppresses the growth of Saccharomyces cerevisiae INVSc1 by disrupting the endolysosomal pathway [0.03%]
天南星科薯蓣提取物通过破坏内溶酶体途径抑制酿酒母菌INVSc1的生长
Sen Takeshita,Hideki Inoue,Yasuhiro Iida
Sen Takeshita
Exploring novel antifungal mechanisms is needed because of the side effects of existing treatments and the increasing prevalence of drug resistance. We recently reported that inhibition of the endocytic pathway via a lysosome inhibitor or F...
Suppression/competition PCR: A novel method to minimize unwanted amplicons in metabarcoding, with applications to parasite detection in fecal samples [0.03%]
一种新颖的方法可减少元条形码中的不需要的扩增子，并将其应用于粪便样本中寄生虫检测领域
Justin Mark Carpani,John R Barta,Rebecca A Guy
Justin Mark Carpani
Metabarcoding is widely used for detecting microorganisms in fecal samples, but its effectiveness is often limited by the co-amplification of abundant non-target DNA. In this study, a novel metabarcoding assay was developed to amplify a nea...
Rapid qualitative identification and application of six common lactic acid bacteria based on surface enhanced Raman spectroscopy [0.03%]
基于表面增强拉曼光谱的六种常见乳酸菌的快速定性鉴别及应用研究
Huihui Zhi,Shijie Liu,Yueyu Bai et al.
Huihui Zhi et al.
To achieve rapid identification and detection of lactic acid bacteria (LAB) from different genera and species, six types of lactic acid bacteria were selected as the research objects, including Lactobacillus plantarum L1, Lactobacillus gass...