Recovery of Escherichia coli and Salmonella enterica from long frozen rectal swabs using enrichment methods in a low-resource setting [0.03%]
在资源匮乏条件下使用富集方法从长期冷冻的直肠棉签样本中分离鉴定大肠杆菌和伤寒沙门氏菌
Hafeez A Adekola,Jacob I Yisau,Ajoke O Adagbada et al.
Hafeez A Adekola et al.
This study evaluated bacterial recovery from rectal swabs stored at -20 °C for one month in Cary Blair medium. Enrichment methods markedly improved detection of Escherichia coli and Salmonella enterica compared to direct plating. Tryptone ...
Fungal false positive in BioFire® FilmArray® analysis for bloodstream infection [0.03%]
BioFire®FilmArray®血流感染检测中真菌假阳性结果的分析
Osamu Imataki,Ryoko Masumoto,Masahiro Hamano et al.
Osamu Imataki et al.
Background: The BioFire FilmArray® system is an automated multiplex PCR tool for detecting pathogens in clinical samples. False positives occasionally occur due to cross-reactivity, contamination, or assay specificity is...
A strategy to enhance detection of Corynebacterium in pus from human non-puerperal mastitis via pre-culture and extended incubation [0.03%]
一种通过预培养和延长培养时间来提高人类非产褥期乳腺炎脓液中棒状杆菌检测率的策略
Nan Zheng,Xidong Gu,Jiangting Yu et al.
Nan Zheng et al.
This study aimed to optimize the detection method for Corynebacterium in pus from patients with non-puerperal mastitis (NPM) to enhance diagnostic accuracy and provide guidance for clinical treatment. A total of 216 pus samples from NPM pat...
Restoration of crude oil-contaminated soil using microbes with degradative and plant-growth-promoting abilities [0.03%]
应用兼具降解和促生性能的微生物修复石油污染土壤
Mukesh Aakula,Mayur Mahindra Kedare,Sanjukta Patra et al.
Mukesh Aakula et al.
Microorganisms with traits such as biosurfactant production, hydrocarbonoclastic ability, and PGP activity can be used to restore the soil health of agricultural fields affected by oil pollution. In this study, the ability of a microbial co...
A comparative review of vector insertion techniques in Saccharomyces cerevisiae [0.03%]
模式酵母菌株的载体构建及转化方法比较研究进展综述
Ritika Gopalakrishnan,Kamala Kannan,Ragul Gunasekaran et al.
Ritika Gopalakrishnan et al.
Saccharomyces cerevisiae, a model organism in genetics and molecular biology has been extensively engineered using various vector insertion techniques. This review compares and contrasts three prominent techniques: In vivo homologous recomb...
Experimental and computational approaches to adaptive viral evolution: Linking molecular variation to phenotypic outcomes [0.03%]
实验和计算适应性病毒进化的方法：将分子变异与表型结果相联系
Yanfeng Huang,Zhiying Ou,Xi Xue et al.
Yanfeng Huang et al.
Viruses pose a persistent global health threat due to their high mutation rates and rapid evolutionary capacity, which drive zoonotic spillover, vaccine escape, and drug resistance. Even single amino acid substitutions might impact viral in...
Validation of a representative sampling protocol for studying grape berry microbiota at the plot scale [0.03%]
在小区尺度上研究葡萄浆果微生物群的代表性取样协议的有效性
Gabriela Pinto Miguel,Olivier Rué,Martine Pradal et al.
Gabriela Pinto Miguel et al.
In viticulture, a comprehensive understanding of the grape microbiota is crucial, as it influences various aspects of the winemaking process, from vine health and grape development to fermentation dynamics and the final sensory profile of t...
Application of newly developed PCR detection and sequencing methods for the lafA gene to Aeromonas isolates [0.03%]
新开发的lafA基因PCR检测和序列分析方法在气单胞菌属分离株中的应用
Kazufumi Miyagi,Noriaki Shimoji,Itaru Hirai
Kazufumi Miyagi
The novel polymerase chain reaction (PCR) and sequencing assays were developed to reveal the prevalence and nucleotide sequence of the lateral flagellar A gene (lafA) in clinical and environmental strains of Aeromonas spp. and were applied ...
Sephadex filtration in conjunction with preputial washing: A strategy to minimize bacterial load and enhance cryo-survival in Murrah buffalo bull semen [0.03%]
联合使用塞弗多克斯过滤和包皮清洗降低水牛冷冻精液的细菌含量及提高解冻精子质量的一种策略
Dipti Nain,T K Mohanty,M Bhakat et al.
Dipti Nain et al.
The presence of bacteria poses a significant challenge to the quality of bovine semen used in artificial insemination. This study was conducted based on the hypothesis that Sephadex reduces the bacterial content of semen, while also separat...
Exploring improved sample recovery and processing methods targeting Bacillus anthracis spores from vegetation matrices [0.03%]
针对植被基质中炭疽芽胞杆菌孢子的改进取样回收及前处理方法研究
Michael Pirhalla,Denise Aslett,Worth Calfee et al.
Michael Pirhalla et al.
Following an accidental or intentional outdoor release of an environmentally persistent biological agent, such as Bacillus anthracis (Ba), a sampling campaign involving the collection of bulk vegetation matrices (grass, ground litter, and l...