Atinuke M Olajide,Gisèle LaPointe
Atinuke M Olajide
Paenibacillus macerans can cause spoilage of milk during extended storage. However, the natural milk microbiota interferes with the enumeration of Paenibacillus species in raw milk. In this study, a qualitative SYBR Green real-time PCR assa...
Biotransformation of 12-hydroxystearic acid to gamma-decalactone: Comparison of two separation systems [0.03%]
12-羟基硬脂酸生物转化法合成γ-十氢萘内酯：两种分离系统的比较研究
Shaofeng Rong,Xinhui Guan,Qianqian Li et al.
Shaofeng Rong et al.
Biotransformation of natural products to the natural flavoring, gamma-decalactone (GDL), has attracted considerable attention. However, improving its yield is challenging due to its high feedback inhibition of yeast cells, which lowers the ...
Multi fragment melting analysis system (MFMAS) for one-step identification of lactobacilli [0.03%]
用于一步鉴定乳酸菌的多片段熔解分析系统(MFMAS)
Zülal Kesmen,Özge Kılıç,Yasin Gormez et al.
Zülal Kesmen et al.
The accurate identification of lactobacilli is essential for the effective management of industrial practices associated with lactobacilli strains, such as the production of fermented foods or probiotic supplements. For this reason, in this...
Influence of PCR cycle number on 16S rRNA gene amplicon sequencing of low biomass samples [0.03%]
PCR循环次数对低生物量样品16S rRNA基因扩增子测序的影响
Monica C Witzke,Alexis Gullic,Peggy Yang et al.
Monica C Witzke et al.
The objective of this study was to evaluate the effects of increased PCR cycle number on sequencing results from samples with low microbial biomass, including bovine milk, and murine pelage and blood. We hypothesized that subjecting DNA fro...
"Sporotan" a new fluorescent stain for identifying cryptic spores of Rhodobacter johrii [0.03%]
"Sporotan"用于鉴定Rhodobacter johrii的隐性孢子的新荧光染料
Ashif Ali,Kumar Gaurav,N Senthilnathan et al.
Ashif Ali et al.
We propose a new fluorescent stain "sporotan" and staining protocol which aid in the identification of cryptic endospores which are otherwise mistaken as poly-β-hydroxyalkanoate granules. ...
An efficient genetic transformation system for Chinese medicine fungus Tolypocladium ophioglossoides [0.03%]
中药真菌 Tolypocladium ophioglossoides 的高效遗传转化系统
Xin-Ai Chen,Xian He,Min Zhang et al.
Xin-Ai Chen et al.
Tolypocladium ophioglossoides is a rare and valuable fungus extensively used in Chinese medicine for relieving postmenopausal syndrome in women yet its bioactive molecules are unknown. To explore its molecular mechanisms, we have developed ...
Avidity of IgG to Rickettsia prowazekii and the presence of specific IgM in blood sera for retrospective analysis of the 1998 epidemic typhus outbreak in Russia [0.03%]
对普氏立克次体IgG的亲和力以及血清中特异性IgM的存在，用于回顾分析俄罗斯1998年流行性斑疹伤寒疫情
Tatiana Chekanova,Stanislav Shpynov
Tatiana Chekanova
The authors applied a new methodological approach based not only on the study of IgM/IgG to Rickettsia prowazekii in sera, but also on the estimation of the avidity index of specific IgG. The data allowed the authors to draw new conclusions...
Five-year cryopreservation at -80 °C of edible and medicinal basidiomycetes by wheat grain technique [0.03%]
小麦谷粒法冻存食用和药用伞菌5年的研究
Lienine Luiz Zaghi Junior,Miria Benetati Delgado Bertéli,Janyeli Dorini Silva de Freitas et al.
Lienine Luiz Zaghi Junior et al.
This research has focused on basidiomycete cryopreservation at -80 °C and developed a cryopreservation method based on the use of hard or medium-hard endosperm wheat grains as a mycelial carrier for cryopreservation. The aim of this study ...
A novel image cytometry-based Lactobacillus bacterial enumeration method for the production of kettle sour beer [0.03%]
新型基于图像细胞计量的乳酸菌计数方法在煮沸前麦汁发酵中的应用研究
Matthew Hodgkin,Suzanne M Purseglove,Leo Li-Ying Chan et al.
Matthew Hodgkin et al.
The demands for a variety of craft beer flavors have been increasing in the United States. To meet this rising demand, breweries have been experimenting with kettle sour beer that utilizes lactic acid-producing bacteria for fermentation. Th...
The impact of delayed analysis of positive blood cultures on the performance of short-term culture followed by MALDI-TOF MS [0.03%]
阳性血培养延迟分析对短期培养后MALDI-TOF MS性能的影响
Alexander T A Johnsson,Alicia Y W Wong,Volkan Özenci
Alexander T A Johnsson
Background: Short-term culture followed by MALDI-TOF MS is one of the most widely used methods for fast identification of microorganisms from blood cultures. The method identifies the vast majority of bloodstream infectio...