Fetal bovine serum enhances recombinant protein secretion and stability in Pichia pastoris [0.03%]
胎牛血清可提高毕赤酵母中重组蛋白的分泌及稳定性
Kenneth L Nguyen,Craig Vierra,David Vang et al.
Kenneth L Nguyen et al.
Background: Komagataella phaffii, formerly known as Pichia pastoris, is a methylotrophic yeast employed as a recombinant protein factory for academic and industrial purposes. Despite its advantages, some recombinant prote...
Stage-dependent association of Vitamin D receptor with macrophage polarization in allergic rhinitis [0.03%]
维生素D受体在过敏性鼻炎中与巨噬细胞极化阶段依赖相关性研究
Yajing Huang,Dingyi Shi,Mengni Zhang et al.
Yajing Huang et al.
Background: Allergic rhinitis (AR) is driven by Th1/Th2 imbalance, with macrophages as key mediators. Vitamin D (VitD) deficiency is associated with AR; however, how VitD regulates macrophage-related molecules remains unc...
Inka Brockhausen,Sriram Neelamegham,Khushi Matta
Inka Brockhausen
Harry Schachter was at the forefront of glycobiology research for more than 60 years. His work led to the discovery of novel enzymes and pathways critical for glycoprotein biosynthesis, exploring glycan functions across various model system...
Matrix metalloproteinase-14 promotes LRP6 shedding in vascular smooth muscle cells and its expression is elevated in the tibial arteries from peripheral artery disease [0.03%]
基质金属蛋白酶-14通过促进血管平滑肌细胞LRP6的脱落来促进血管钙化及其在动脉疾病胫骨动脉中的表达升高
Hui-Guang Chen,Di Gao,Gui-Feng Zeng et al.
Hui-Guang Chen et al.
Vascular smooth muscle cells (VSMCs) dedifferentiation is a key driver of peripheral artery disease (PAD), a common complication of atherosclerosis. Although matrix metallopeptidase 14 (MMP14) has been implicated in promoting VSMC dediffere...
Importance of N-terminal P2 residue in substrate recognition by bacterial DPP7 activity revealed by coefficient of variation in protease activity [0.03%]
细菌DPP7活性的变异系数揭示底物识别中N-末端P2残基的重要性
Momo Sawase,Kana Shirakura,Yuko Ohara-Nemoto et al.
Momo Sawase et al.
Dipeptidyl-peptidases (DPPs), including the S9-family DPP4 and DPP5 and the S46-family DPP7 and DPP11, are essential for growth of Porphyromonas gingivalis, a periodontopathic bacterium associated with systemic diseases. DPPs release dipept...
Complex anionic and zwitterionic N-glycans in Drosophila melanogaster [0.03%]
果蝇复合阴离子及两性离子N-糖链结构复杂且多样
Kristína Maďarová,Zuzanna Dutkiewicz,Lena Nuschy et al.
Kristína Maďarová et al.
Drosophila melanogaster is arguably the most widely-employed invertebrate model organism; here we have focussed on its anionic N-glycome applying our off-line HPLC-MALDI-TOF-MS approach. Using MS/MS in combination with chemical and enzymati...
Structure and biosynthetic mechanisms of galactomannans in filamentous fungi [0.03%]
丝状真菌半乳甘露聚糖的结构及其生物合成机制
Takuji Oka,Chihiro Kadooka,Yutaka Tanaka et al.
Takuji Oka et al.
The cell wall of filamentous fungi is crucial for the physical protection of the cell, the sensing environmental cues, and the expression of virulence. The fungal cell wall consists of a rigid yet flexible three-dimensional network in which...
Reduced adverse effects of infrared free electron laser-irradiated insulin amyloid in vitro and in mice [0.03%]
体内和小鼠中近红外自由电子激光辐照的胰岛素淀粉样体的副作用减少
Akiko Katano-Toki,Fumio Hayashi,Hiroshi Takahashi et al.
Akiko Katano-Toki et al.
Insulin is a hormone that physiologically contributes to the control of glucose metabolism and therefore insulin self-injection has been one of the highly effective therapeutic approaches for diabetes. However, repeated injection of insulin...
High-dose vitamin C preferentially exerts a sustained growth-inhibitory effect in human tongue carcinoma cells over dysplastic oral keratinocytes [0.03%]
大剂量维生素C对人类舌癌细胞的持续生长抑制作用优先于异型增生口腔角质细胞
Yasukazu Saitoh,Takuya Ishikawa,Mai Yamamoto et al.
Yasukazu Saitoh et al.
The effects of ascorbic acid (AsA) on human tongue cancer cells have been reported; however, anticancer mechanisms and preferential effects in carcinoma cells compared with those in dysplastic keratinocytes remain unclear. Here, we investig...
SSD: Targeting inflammasome and oxidative stress as a therapeutic strategy in inflammatory diseases [0.03%]
靶向炎症小体和氧化应激作为治疗炎症性疾病的策略
Yukai Zhao,Xinyu Fu,Hongli Zuo et al.
Yukai Zhao et al.
Objective and design: This study systematically investigated the therapeutic efficacy of SSD, a stigmasterol derivative with H₂S-releasing properties, against NLRP3 inflammasome activation using both in vitro and in vivo...