Da-Kang Yao,Jin-Yu Shao
Da-Kang Yao
In cellular and molecular biomechanics, extensional stiffness of rod-like structures such as leukocyte microvilli can be easily measured with many techniques, but not many techniques are available for measuring their flexural stiffness. In ...
Stretch and Shear Interactions Affect Intercellular Junction Protein Expression and Turnover in Endothelial Cells [0.03%]
拉伸和剪切相互作用影响内皮细胞间连接蛋白的表达和周转
Danielle E Berardi,John M Tarbell
Danielle E Berardi
Complex hemodynamics play a role in the localization and development of atherosclerosis. Endothelial cells (ECs) lining blood vessel walls are directly influenced by various hemodynamic forces: simultaneous wall shear stress (WSS), normal s...
Intracellular Flow Cytometric Measurement of Extracellular Matrix Components in Porcine Intervertebral Disc Cells [0.03%]
猪椎间盘细胞细胞外基质成分的流式细胞术测定
Daniel J Flagler,Chun-Yuh Huang,Tai-Yi Yuan et al.
Daniel J Flagler et al.
The objective of this study was to develop and demonstrate the utility of a novel method of evaluating intracellular levels of extracellular matrix (ECM) components in intervertebral disc (IVD) cells using flow cytometry. By using this meth...
Cyclic Hydraulic Pressure and Fluid Flow Differentially Modulate Cytoskeleton Re-Organization in MC3T3 Osteoblasts [0.03%]
周期性液压压力和流体流动对MC3T3成骨细胞细胞骨架重构的协同与拮抗作用
Joseph D Gardinier,Shyama Majumdar,Randall L Duncan et al.
Joseph D Gardinier et al.
Mechanical loads are essential towards maintaining bone mass and skeletal integrity. Such loads generate various stimuli at the cellular level, including cyclic hydraulic pressure (CHP) and fluid shear stress (FSS). To gain insight into the...
Dynamics of Microvillus Extension and Tether Formation in Rolling Leukocytes [0.03%]
滚动白细胞中的微绒毛伸展和牵拉的动力学过程研究
Maria K Pospieszalska,Klaus Ley
Maria K Pospieszalska
P-selectin glycoprotein ligand-1 (PSGL-1) binding to P-selectin mediates leukocyte rolling under conditions of flow. In human neutrophils, a type of leukocyte belonging to the innate immune system, PSGL-1 molecules are located on the neutro...
Proteomic Profiling of Mesenchymal Stem Cell Responses to Mechanical Strain and TGF-beta1 [0.03%]
机械拉伸和TGF-β1诱导的间充质干细胞蛋白质组学特征分析
Kyle Kurpinski,Julia Chu,Daojing Wang et al.
Kyle Kurpinski et al.
Mesenchymal stem cells (MSCs) are a potential source of smooth muscle cells (SMCs) for constructing tissue-engineered vascular grafts. However, the details of how specific combinations of vascular microenvironmental factors regulate MSCs ar...
Shear Stress Regulates the Flk-1/Cbl/PI3K/NF-κB Pathway Via Actin and Tyrosine Kinases [0.03%]
剪切应力通过肌动蛋白和酪氨酸激酶调节Flk-1/Cbl/PI3K/NF-κB途径
Yingxiao Wang,Leona Flores,Shaoying Lu et al.
Yingxiao Wang et al.
Vascular endothelial cells (ECs) are continuously exposed to mechanical stimuli (e.g., shear stress). Our previous study has shown that the shear-induced nuclear factor-κB (NF-κB) activation is mediated by integrins [Bhullar, I. S., Y. S....
Dependence of zonal chondrocyte water transport properties on osmotic environment [0.03%]
软骨细胞渗透环境对其区间水运输性质的影响分析
Elizabeth S Oswald,Pen-Hsiu Grace Chao,J Chloe Bulinski et al.
Elizabeth S Oswald et al.
OBJECTIVE: The increasing concentration of proteoglycans from the surface to the deep zone of articular cartilage produces a depth-dependent gradient in fixed charge density, and therefore extracellular osmolarity, which may vary with loadi...
Yanan Du,Edward Lo,Mahesh K Vidula et al.
Yanan Du et al.
The paper describes a protocol to fabricate cell-laden microgel assemblies with pre-defined micro-architecture and complexity by a bottom-up approach, which can be used for tissue engineering applications. The assembly process was driven by...
Pericellular Matrix Mechanics in the Anulus Fibrosus Predicted by a Three-Dimensional Finite Element Model and In Situ Morphology [0.03%]
三维有限元模型和原位形态预测的纤维环细胞外基质力学性质
Li Cao,Farshid Guilak,Lori A Setton
Li Cao
Anulus fibrosus (AF) cells have been demonstrated to exhibit dramatic differences in morphology and biologic responses to different types of mechanical stimuli. AF cells may reside as single cell, paired or multiple cells in a contiguous pe...