Jeremy H Snook,William H Guilford
Jeremy H Snook
Molecular dissociation rates have long been known to be sensitive to applied force. We use a laser trap to provide evidence that rates of association may also be force-dependent. We use the thermal fluctuation assay to study single bonds be...
Stochastic Dynamics of Membrane Protrusion Mediated by the DOCK180/Rac Pathway in Migrating Cells [0.03%]
迁移细胞中DOCK180/Rac信号途径介导的膜突起的随机动力学过程研究
Erik S Welf,Jason M Haugh
Erik S Welf
Cell migration is regulated by processes that control adhesion to extracellular matrix (ECM) and force generation. While our fundamental understanding of how these control mechanisms are actuated at the molecular level (signal transduction)...
Model Convolution: A Computational Approach to Digital Image Interpretation [0.03%]
模型卷积：数字图像解读的计算方法
Melissa K Gardner,Brian L Sprague,Chad G Pearson et al.
Melissa K Gardner et al.
Digital fluorescence microscopy is commonly used to track individual proteins and their dynamics in living cells. However, extracting molecule-specific information from fluorescence images is often limited by the noise and blur intrinsic to...
Coarse-Grained Structural Modeling of Molecular Motors Using Multibody Dynamics [0.03%]
多刚体动力学在分子马达的粗粒度结构建模中的应用研究
David Parker,Zev Bryant,Scott L Delp
David Parker
Experimental and computational approaches are needed to uncover the mechanisms by which molecular motors convert chemical energy into mechanical work. In this article, we describe methods and software to generate structurally realistic mode...
Application of Population Dynamics to Study Heterotypic Cell Aggregations in the Near-Wall Region of a Shear Flow [0.03%]
应用群体动力学研究剪切流近壁区域异质细胞聚集现象
Yanping Ma,Jiakou Wang,Shile Liang et al.
Yanping Ma et al.
Our research focused on the polymorphonuclear neutrophils (PMNs) tethering to the vascular endothelial cells (EC) and the subsequent melanoma cell emboli formation in a shear flow, an important process of tumor cell extravasation from the c...
P Shi,K Shen,S Ghassemi et al.
P Shi et al.
Mechanical cues may have important roles in tissue morphogenesis; progression through complex functions like differentiation may be associated with changes in cellular force generation and mechanosensing. To explore this concept, we use ela...
Mechanical Stability Determines Stress Fiber and Focal Adhesion Orientation [0.03%]
力学稳定性决定应力纤维和焦斑粘附的取向
Dimitrije Stamenović,Konstantinos A Lazopoulos,Athanassios Pirentis et al.
Dimitrije Stamenović et al.
It is well documented in a variety of adherent cell types that in response to anisotropic signals from the microenvironment cells alter their cytoskeletal organization. Previous theoretical studies of these phenomena were focused primarily ...
Internalization of Formyl Peptide Receptor in Leukocytes Subject to Fluid Stresses [0.03%]
流体应力下白细胞中肽酰基肽受体的内化
Susan S Su,Geert W Schmid-Schönbein
Susan S Su
Human leukocytes retract pseudopods under normal physiologic levels of fluid shear stress even in the absence of any other mediator. To gain more detailed understanding of the mechanisms that regulate this cell behavior, we exposed leukocyt...
Lateral Mobility of E-cadherin Enhances Rac1 Response in Epithelial Cells [0.03%]
E-钙黏蛋白侧向扩散增强上皮细胞中Rac1的反应
J Tsai,L C Kam
J Tsai
The fluidity of cellular membranes imparts lateral mobility of proteins across the cell surface. To understand the impact of lateral mobility on cell-cell communication, a protein consisting of the extracellular recognition domains of E-cad...
Validation, In-Depth Analysis, and Modification of the Micropipette Aspiration Technique [0.03%]
微管吸取技术的验证、深入分析及改进方法研究
Yong Chen,Baoyu Liu,Gang Xu et al.
Yong Chen et al.
The micropipette aspiration technique (MAT) has been successfully applied to many studies in cell adhesion such as leukocyte-endothelium interactions. However, this technique has never been validated experimentally and it has been only empl...