Manipulation of Suspended Single Cells by Microfluidics and Optical Tweezers [0.03%]
基于微流控技术和光镊的单细胞操作技术
Nathalie Nève,Sean S Kohles,Shelley R Winn et al.
Nathalie Nève et al.
Chondrocytes and osteoblasts experience multiple stresses in vivo. The optimum mechanical conditions for cell health are not fully understood. This paper describes the optical and microfluidic mechanical manipulation of single suspended cel...
The Role of Primary Cilia in Mesenchymal Stem Cell Differentiation: A Pivotal Switch in Guiding Lineage Commitment [0.03%]
主细胞纤毛在间充质干细胞分化中的作用：指引谱系决定的关键转换点
Padmaja Tummala,Emily J Arnsdorf,Christopher R Jacobs
Padmaja Tummala
Primary cilia are sensory organelles that have been shown to play a critical role in lineage commitment. It was our hypothesis that the primary cilium is necessary for chemically induced differentiation of human mesenchymal stem cells (MSC)...
Modeling of B cell Synapse Formation by Monte Carlo Simulation Shows That Directed Transport of Receptor Molecules Is a Potential Formation Mechanism [0.03%]
采用蒙特卡罗模拟法建立B细胞突触模型表明受体分子定向运输是其形成的潜在机制
Philippos K Tsourkas,Subhadip Raychaudhuri
Philippos K Tsourkas
The formation of the protein segregation structure known as the "immunological synapse" in the contact region between B cells and antigen presenting cells appears to precede antigen (Ag) uptake by B cells. The mature B cell synapse consists...
Duty Cycle of Deformational Loading Influences the Growth of Engineered Articular Cartilage [0.03%]
变形载荷的占空比影响工程软骨的增长
Kenneth W Ng,Robert L Mauck,Christopher C-B Wang et al.
Kenneth W Ng et al.
This study examines how variations in the duty cycle (the duration of applied loading) of deformational loading can influence the mechanical properties of tissue engineered cartilage constructs over one month in bioreactor culture. Dynamic ...
Current-Controlled Electrical Point-Source Stimulation of Embryonic Stem Cells [0.03%]
胚胎干细胞的电流控制型电点刺激源模拟研究
Michael Q Chen,Xiaoyan Xie,Kitchener D Wilson et al.
Michael Q Chen et al.
Stem cell therapy is emerging as a promising clinical approach for myocardial repair. However, the interactions between the graft and host, resulting in inconsistent levels of integration, remain largely unknown. In particular, the influenc...
Optimization by Response Surface Methodology of Confluent and Aligned Cellular Monolayers for Nerve Guidance [0.03%]
响应曲面法优化神经导向的汇合和对齐细胞单层
Celinda M Kofron,Diane Hoffman-Kim
Celinda M Kofron
Anisotropic tissue structures provide guidance for navigating neurons in vitro and in vivo. Here we optimized the generation of comparable anisotropic monolayers of astrocytes, endothelial cells, and Schwann cells as a first step toward det...
Mechanical properties and gene expression of chondrocytes on micropatterned substrates following dedifferentiation in monolayer [0.03%]
单层培养去分化后微图案基底上软骨细胞力学性能及基因表达特征的研究
Eric M Darling,Poston E Pritchett,Benjamin A Evans et al.
Eric M Darling et al.
Chondrocytes in articular cartilage normally exhibit high expression of collagen II and aggrecan but rapidly dedifferentiate to a fibroblastic phenotype if passaged in culture. Previous studies have suggested that the loss of chondrocyte ph...
Shamik Sen,Adam J Engler,Dennis E Discher
Shamik Sen
Many tissue cells exert contractile forces that mechanically couples them to elastic matrices and that influence cell adhesion, cytoskeletal organization, and even cell differentiation. However, strains within the depths of matrices are oft...
Stabilization of the spectrin-like domains of nesprin-1α by the evolutionarily conserved "adaptive" domain [0.03%]
进化上保守的“适应性”结构域稳定nesprin-1α上的类似spectrin的结构域
Zhixia Zhong,Siwei A Chang,Agnieszka Kalinowski et al.
Zhixia Zhong et al.
Nesprins are located at the outer and inner membranes of the nuclear envelope and help link the cytoskeleton to the nucleoskeleton. Nesprin-1α, located at the inner nuclear membrane, binds to A-type lamins and emerin and has homology to sp...
INSIGHTS INTO THE ROLES OF NON-MUSCLE MYOSIN IIA IN HUMAN KERATINOCYTE MIGRATION [0.03%]
人表皮细胞迁移中非肌肉类型ⅡA肌球蛋白作用的研究进展
Saheli Sarkar,Thomas Egelhoff,Harihara Baskaran
Saheli Sarkar
Epidermal cell migration is a key factor in wound healing responses, regulated by the F-actin-myosin II systems. Previous reports have established the importance of non-muscle myosin II (NMII) in regulating cell migration. However, the role...