Jihye Seong,Shaoying Lu,Yingxiao Wang
Jihye Seong
The Src/FAK complex is involved in many signaling pathways and plays crucial roles in cell adhesion/migration. It becomes clear that the subcellular localization of Src and FAK is crucial for their activities and functions. In this article,...
Randi L Saunders,Daniel A Hammer
Randi L Saunders
Angiogenesis is the process by which endothelial cells grow and disassemble into functional blood vessels. In this study, we examine the fundamental processes that control the assembly of endothelial cells into networks in vitro. Network as...
A Model for Stress Fiber Realignment Caused by Cytoskeletal Fluidization During Cyclic Stretching [0.03%]
细胞周期拉伸期间细胞骨架流体化引起的应力纤维重新排列的模型
Athanassios P Pirentis,Elizabeth Peruski,Andreea L Iordan et al.
Athanassios P Pirentis et al.
Uniaxial cyclic substrate stretching results in a concerted change of cytoskeletal organization such that stress fibers (SFs) realign away from the direction of stretching. Recent experiments revealed that brief transient stretch promptly a...
A Semi-Automatic Method for Image Analysis of Edge Dynamics in Living Cells [0.03%]
活细胞边缘动力学图像分析的半自动方法
Lawrence Huang,Brian P Helmke
Lawrence Huang
Spatial asymmetry of actin edge ruffling contributes to the process of cell polarization and directional migration, but mechanisms by which external cues control actin polymerization near cell edges remain unclear. We designed a quantitativ...
Real Time FRET Based Detection of Mechanical Stress in Cytoskeletal and Extracellular Matrix Proteins [0.03%]
基于FRET的细胞骨架和基质蛋白机械应力实时检测技术
Fanjie Meng,Thomas M Suchyna,Elena Lazakovitch et al.
Fanjie Meng et al.
A molecular force sensing cassette (stFRET) was incorporated into actinin, filamin, and spectrin in vascular endothelial cells (BAECs) and into collagen-19 in Caenorhabditis elegans. To estimate the stress sensitivity of stFRET in solution,...
Difference in Energy Metabolism of Annulus Fibrosus and Nucleus Pulposus Cells of the Intervertebral Disc [0.03%]
椎间盘髓核和纤维环细胞能量代谢的区别研究进展
Jessica Czamanski Salvatierra,Tai Yi Yuan,Hanan Fernando et al.
Jessica Czamanski Salvatierra et al.
Low back pain is associated with intervertebral disc degeneration. One of the main signs of degeneration is the inability to maintain extracellular matrix integrity. Extracellular matrix synthesis is closely related to production of adenosi...
Stepwise increasing and decreasing fluid shear stresses differentially regulate the functions of osteoblasts [0.03%]
逐步增加和降低的流体剪切应力通过不同的机制调节成骨细胞功能
Jun Pan,Tingxiu Zhang,Li Mi et al.
Jun Pan et al.
It is well accepted that osteoblasts respond to fluid shear stress (FSS) depending on the loading magnitude, rate, and temporal profiles. Although in vivo observations demonstrated that bone mineral density changes as the training intensity...
Fluorescence Resonance Energy Transfer (FRET)-based Detection of Profilin-VASP Interaction [0.03%]
基于荧光共振能量转移(FRET)的Profilin-VASP相互作用检测方法
Dave Gau,Zhijie Ding,Catherine Baty et al.
Dave Gau et al.
Profilins belong to a family of small G-actin binding proteins which are thought to assist in F-actin elongation at the leading edge of migrating cells through their interactions with a host of actin-binding proteins including Ena (enabled)...
Insights into the Mechanical Properties of the Kinesin Neck Linker Domain from Sequence Analysis and Molecular Dynamics Simulations [0.03%]
序列分析和分子动力学模拟揭示了驱动蛋白颈连接区的机械性质
Venkatesh Hariharan,William O Hancock
Venkatesh Hariharan
The 14-18 amino acid kinesin neck linker domain links the core motor to the coiled-coil dimerization domain. One puzzle is that the neck linker appears too short for the 4 nm distance each linker must stretch to enable an 8 nm step - when m...
Signaling and Dynamics of Activation of LFA-1 and Mac-1 by Immobilized IL-8 [0.03%]
通过固定化IL-8激活LFA-1和Mac-1的信号传导及动力学研究
Elena B Lomakina,Richard E Waugh
Elena B Lomakina
The dynamic response of neutrophils to interleukin-8 (IL-8) is of central interest in inflammation. Chemokine -induced β(2) integrin dependent adhesion can take several minutes after initial contact with IL-8 as evidenced by increased cell...