Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus [0.03%]
一种新型针对伪狂犬病毒gN基因分型的Taqman-qPCR检测方法的建立及应用研究
Yulan Jin,Xuan Ji,Weiren Dong et al.
Yulan Jin et al.
Pseudorabies virus (PRV), known as porcine herpesvirus type I, belongs to the α-herpesvirus subfamily of the herpesviridae family. Its virions are oval or round, with a genome of approximately 143 000 bp and a high guanine-cytosine (GC) co...
A novel A-to-G mutation in circBDP1 alters adipocyte proliferation and differentiation and affects bovine carcass traits [0.03%]
circBDP1中的一种新的A到G突变改变了脂肪细胞的增殖和分化并影响了肉牛胴体性状
Enhui Jiang,Chiyuan Zhang,Zhuoyuan He et al.
Enhui Jiang et al.
Fat deposition in cattle, influenced by factors such as genetics, age, sex, and nutrition, plays a crucial role in determining growth rates, beef yield, and meat quality. Among these, genetics is the predominant factor (Kim et al., 2020; Xi...
Neuroprotection of engineered Clostridium butyricum-pMTL007-GLP-1 in A53T α-synuclein (α-syn) mouse model via PI3K/AKT/GSK-3β [0.03%]
工程化产丁酸芽孢杆菌-pMTL007-GLP-1在A53T α-突触核蛋白(α-syn)小鼠模型中的神经保护作用及PI3K/AKT/GSK-3β相关机制研究
Xin Fang,Yun Wang,Zhenli Long et al.
Xin Fang et al.
Parkinson's disease (PD) is a prevalent neurodegenerative disorder with limited therapeutic options and no cure, underscoring the urgent need for novel treatment strategies. Our previous work demonstrated that an engineered strain of Clostr...
Selenocystine-based fluorescence assay combined with structural prediction for functional analysis of cystine transporter variants in cystinuria [0.03%]
基于硒半胱氨酸的荧光测定法结合结构预测用于半胱胺转运蛋白变异体的功能分析及半胱胺尿症研究
Xiaobai He,Xinyi Qian,Xiaoguang Zheng et al.
Xiaobai He et al.
Objectives: Cystine stones account for 1%‒2% of adult and up to 10% of pediatric kidney stones. They result from cystinuria, an autosomal recessive disorder caused by mutations in solute carrier family 3 member 1 (SLC3A1...
Machine learning-driven evaluation of protein kinase D3 as a co-diagnostic biomarker in hepatocellular carcinoma [0.03%]
机器学习驱动的蛋白激酶D3作为肝细胞癌共诊断生物标志物的评估
Jing Li,Yifan Zhao,Yicheng Ma et al.
Jing Li et al.
To elucidate the diagnostic value and clinical relevance of protein kinase D3 (PRKD3) in hepatocellular carcinoma (HCC), we analyzed data retrieved from The Cancer Genome Atlas (TCGA) database, which revealed high expression of PRKD3 in HCC...
DNA-based one-pot rapid biosensing platform integrating turn-back loop primer-accelerated LAMP and Pyrococcus furiosus Argonaute for meat visual authentication [0.03%]
基于DNA的一锅快速生物传感平台集成发夹启动子加速LAMP和Pyrococcus furiosus Argonaute肉品可视化认证
Sheng Ding,Jing Li,Minglong Yang et al.
Sheng Ding et al.
Meat adulteration is a significant global food safety challenge, creating a pressing need for rapid and on-site detection technologies. Herein, we present an intelligent one-pot biosensing platform termed one-pot TLAMP-PfAgo assay (OTPA) th...
Dual-functional nanoplatform for simultaneous degradation of circRNA CDR1as and real-time monitoring of miR-7 in live cells [0.03%]
一种用于活细胞中同时降解circRNA CDR1as和实时监测miR-7的双重功能型纳米平台
Yan Huang,Jialin Ye,Lan Xu et al.
Yan Huang et al.
Circular RNAs (circRNAs) are key post-transcriptional regulators with critical roles in pathogenesis, yet existing tools for their precise manipulation and functional analysis in living cells remain to be developed. A compelling therapeutic...
Advances in biomarkers for Parkinson's disease: from molecular pathology to precision diagnostics [0.03%]
帕金森病生物标志物研究进展：从分子病理学到精准诊断
Xiang Li,Honglin Chen,Xu Wang et al.
Xiang Li et al.
Parkinson's disease (PD) is the second most common neurodegenerative disorder, and continues to present significant challenges in early diagnosis, precise subtyping, and prognosis assessment. In recent years, the field of biomarker research...
Blood‒brain barrier permeability assessment: from biomimetic models to multimodal imaging [0.03%]
从仿生模型到多模式成像的血脑屏障通透性评价方法评估
Miaomiao Wang,Lu Gan,Yiru Fan et al.
Miaomiao Wang et al.
The blood‒brain barrier (BBB) is a vital physiological structure that maintains the microenvironmental homeostasis in the central nervous system (CNS). Imbalances in its permeability play a key role in various neurological disorders, inclu...
Programmable DNAzyme nanocatalysts for tumor immunometabolic modulation [0.03%]
可用于肿瘤免疫代谢调控的DNA酶纳米催化剂
Rongping Luo,Zhuojia Tang,Mengqi Ding et al.
Rongping Luo et al.
Programmable RNA-cleaving DNAzymes (RCDs) represent a unique class of catalytic nucleic acids that couple molecular recognition with enzyme-like activity. While DNAzymes have traditionally been explored for targeted gene regulation, recent ...