Spatial transcriptomics and proteomics technologies for deconvoluting the tumor microenvironment [0.03%]
空间转录组学和蛋白质组学技术破解肿瘤微环境
Nan Wang,Xia Li,Rongshui Wang et al.
Nan Wang et al.
The tumor microenvironment (TME) harbors heterogeneous contents and plays critical roles in tumorigenesis, metastasis, and drug resistance. Therefore, the deconvolution of the TME becomes increasingly essential to every aspect of cancer res...
Ribozyme-processed guide RNA enhances virus-mediated plant genome editing [0.03%]
核糖酶加工向导RNA可增强病毒介导的植物基因组编辑
Youngbin Oh,Hyeonjin Kim,Hyo-Jun Lee et al.
Youngbin Oh et al.
In virus-induced gene-editing system, subgenomic promoters have been used to express guide RNAs (gRNAs). However, the transcription initiation site of the subgenomic promoters remains elusive. Here, we examined the sequence of gRNAs express...
Prevention of nosocomial transmission and biofilm formation on novel biocompatible antimicrobial gloves impregnated with biosynthesized silver nanoparticles synthesized using Eucalyptus citriodora leaf extract [0.03%]
利用柠檬香桃叶提取物生物合成银纳米颗粒的新型生物相容性抗菌手套预防院内传播和生物膜形成
Supakit Paosen,Sakkarin Lethongkam,Suttiwan Wunnoo et al.
Supakit Paosen et al.
Failure in the prevention of cross-transmission from contaminated gloves has been recognized as an important factor that contributes to the spread of several healthcare-associated infections. Ex situ coating process with silver nanoparticle...
Advances in production and structural derivatization of the promising molecule ursolic acid [0.03%]
乌索酸的生产及结构衍生化研究进展
Hao-Ran Liu,Nadeem Ahmad,Bo Lv et al.
Hao-Ran Liu et al.
Ursolic acid (UA) is a ursane-type pentacyclic triterpenoid compound, naturally produced in plants via specialized metabolism and exhibits vast range of remarkable physiological activities and pharmacological manifestations. Owing to signif...
Microaerobic growth-decoupled production of α-ketoglutarate and succinate from xylose in a one-pot process using Corynebacterium glutamicum [0.03%]
以安氏芽孢杆菌一次性联合生产α-酮戊二酸和琥珀酸
Niklas Tenhaef,Jannick Kappelmann,Arabel Eich et al.
Niklas Tenhaef et al.
Background: Lignocellulosic biomass is the most abundant raw material on earth. Its efficient use for novel bio-based materials is essential for an emerging bioeconomy. Possible building blocks for such materials are the ...
Using lysozyme amyloid fibrils as a means of scavenging aggregation-inhibiting compounds [0.03%]
利用溶菌酶淀粉样纤维作为聚集抑制化合物的清除剂
Mantas Ziaunys,Kamile Mikalauskaite,Andrius Sakalauskas et al.
Mantas Ziaunys et al.
The aggregation of amyloidogenic proteins is linked to several amyloidoses, including neurodegenerative disorders, such as Alzheimer's or Parkinson's disease. Currently there are very few effective cures or treatments available, despite cou...
Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation [0.03%]
采用宿主选择和氯酸钠补料调节CHO细胞培养中重组抗体酪氨酸硫酸化修饰水平
Ren Liu,Yixiao Zhang,Amit Kumar et al.
Ren Liu et al.
Background: Tyrosine sulfation is a post-translational modification found on many surface receptors and plays an important role in cell-cell and cell-matrix interactions. However, tyrosine sulfation of therapeutic antibod...
Spinal cord regeneration: A brief overview of the present scenario and a sneak peek into the future [0.03%]
脊髓再生：现状概览及未来展望
Ekta Srivastava,Anamika Singh,Ashok Kumar
Ekta Srivastava
The central nervous system (CNS) portrays appreciable complexity in developing from a neural tube to controlling major functions of the body and orchestrated co-ordination in maintaining its homeostasis. Any insult or pathology to such an o...
An improved lentiviral system for efficient expression and purification of β-defensins in mammalian cells [0.03%]
一种改进的慢病毒系统：在哺乳动物细胞中高效表达和纯化β-防御素肽
Shiraz Ahmed,Jiang Tao,Miaochen Wang et al.
Shiraz Ahmed et al.
β-Defensins are a family of conserved small cationic antimicrobial peptides with different significant biological functions. The majority of mammalian β-defensins are expressed in epididymis, and many of them are predicted to have post-tr...
Stable overexpression of mutated PTEN in Chinese hamster ovary cells enhances their performance and therapeutic antibody production [0.03%]
表达突变型PTEN的中国地鼠卵巢细胞性能和单克隆抗体产量增强
Qin Zhou,Yujie Zhang,Xiaoxiang Lu et al.
Qin Zhou et al.
Chinese hamster ovary (CHO) cells with a high viable cell density (VCD), resilience to culture stress, and the capacity to continuously express recombinant proteins are highly desirable. Phosphatase and tension homology deleted on chromosom...