Scalable Purification of Cowpea Chlorotic Mottle Virus (CCMV) and Its Bioconjugates From Plant Extracts Using Ultrafiltration [0.03%]
利用超滤从植物提取物中规模化纯化Cowpea花叶 mosaic病毒及其生物缀合物
Patrick Opdensteinen,Kyle Lewin,Nicole F Steinmetz
Patrick Opdensteinen
Plant virus-derived materials are finding applications in science, engineering, and technology-with some candidates advancing toward commercialization, scalable manufacturing methods are needed. We recently established ultrafiltration/diafi...
A Pull-Block Metabolic Engineering Strategy for High-Yield and High-Purity Hyoscyamine Production in Atropa belladonna [0.03%]
一种用于高产量和高纯度阿托品生物合成的牵制模块代谢工程策略
Lingjiang Zeng,Hongting Chen,Mengling Wen et al.
Lingjiang Zeng et al.
Hyoscyamine, an active tropane alkaloid primarily extracted from the medicinal plant Atropa belladonna, exhibits analgesic and sedative effects and is widely used as a precursor in pharmaceutical formulations. However, the low yield of hyos...
Metabolic Engineering of Plasmid-Free Escherichia coli for the Highly Efficient Biosynthesis of cis,cis-Muconic Acid From Glucose [0.03%]
利用葡萄糖高效生物合成顺式-穆康酸的无质粒大肠杆菌代谢工程菌株构建及性能研究
Chengbin Wang,Jingxin Yin,Yali Ban et al.
Chengbin Wang et al.
Cis,cis-muconic acid (ccMA) is a promising platform chemical for the production of polymers and fine chemicals. However, conventional synthesis suffers from poor selectivity and environmental concerns, while plasmid-dependent biosynthesis r...
The Convergence of Nanoscience and Stem Cells: A New Era in Cancer Therapeutics [0.03%]
纳米科学与干细胞的 converge：癌症治疗的新时代
Akshaya Priya Raveendran,Ragavendran G,Reya Rene Philip et al.
Akshaya Priya Raveendran et al.
Cancer remains one of the leading causes of global mortality, yet its treatment is frequently limited by poor drug distribution, systemic toxicity, and therapeutic resistance. Stem cells with their self-renewal capacity, lineage-specific di...
Mechanistic Insights Into the Retention and Separation Mechanism of Poly(Ethylene Glycol)-Modified Short Oligonucleotides in Anion-Exchange Chromatography [0.03%]
聚乙二醇修饰的短寡核苷酸在阴离子交换色谱保留和分离机理的见解
Noriko Yoshimoto,Tomoya Matsumoto,Yoshiatsu Ono et al.
Noriko Yoshimoto et al.
Purification is a critical step in the development of synthetic short DNA for pharmaceutical applications. PEGylation enhances stability and pharmacokinetics but introduces steric and hydration effects that affect chromatographic behavior w...
Spatiotemporal Evolution of Malignant Hepatocyte Clones Unveils Immune Evasion Features and Therapeutic Vulnerabilities in Hepatocellular Carcinoma [0.03%]
恶性肝细胞克隆的时空演化揭示了肝癌的免疫逃逸特征和治疗弱点
Shizheng Xiong,Dekang Ren,Jiaming Jin et al.
Shizheng Xiong et al.
Hepatocellular carcinoma (HCC), a highly lethal disease often developing in the context of chronic liver disease, is marked by high relapse rates and low 5-year survival. To investigate the multicellular ecosystem and molecular features of ...
Applications of Hyaluronic Acid in Pharmaceuticals, Healthcare and Cosmetics and Its Biosynthesis [0.03%]
透明质酸在医药、健康和化妆品行业的应用及其生物合成
Seyedali Mousavi,Divakar Dahiya,Poonam Singh Nigam et al.
Seyedali Mousavi et al.
Hyaluronic acid (HA) is an important homopolysaccharide also known as a natural anionic mucopolysaccharide or glycosaminoglycan formed by the alternate connection of glucuronic acid and N-acetylglucosamine as disaccharide units. For this re...
Mechanisms and Therapeutic Potential of Stem Cell-Derived Extracellular Vesicles as Nanocarriers in Musculoskeletal Disorders [0.03%]
间充质干细胞源性外泌体在骨科疾病治疗中的机制和应用前景研究进展
Mengran Qin,Guang Li,Yan Wang et al.
Mengran Qin et al.
The musculoskeletal system consists of bones, joint cartilage, tendons, ligaments, muscles, and their associated nerves and blood vessels. These components work together to maintain human movement and mechanical stability, serving as the cr...
Engineering of High-Yield Recombinant Adeno-Associated Virus Producer Plasmids [0.03%]
高效重组腺相关病毒制备质粒的构建
Marco T Radukic,Dinh To Le,Robert Freudenberg et al.
Marco T Radukic et al.
Recombinant Aadeno-associated virus (rAAV) production lags demand with respect to quality and quantity. We report insights from producer plasmid engineering aimed at increasing yield and homogeneity of rAAV vectors obtained by HEK-293 tripl...
Advanced Scaffold-Guided Stem Cell Therapies for Myocardial Regeneration: Integrating Biomaterials, Cell Engineering, and Smart Delivery Systems [0.03%]
先进的支架引导干细胞治疗心肌再生：整合生物材料、细胞工程和智能递送系统
Khadije Yousefi,Ali Zareian Jahromi,Rozhin Kasiri et al.
Khadije Yousefi et al.
Myocardial infarction remains a leading cause of mortality worldwide, primarily due to the limited regenerative capacity of adult cardiac tissue. Recent advances in regenerative medicine aim to address this limitation through stem cell ther...