Nanoparticle-Based Radiosensitization in Breast Cancer: A Multimodal Approach to Therapy and Immune Regulation [0.03%]
基于纳米颗粒的放射增敏在乳腺癌治疗和免疫调节中的多模态方法研究
Saleh A S AlAbdulhadi,Ali A Majeed,Djamila Polatova et al.
Saleh A S AlAbdulhadi et al.
Radiotherapy is essential for breast cancer treatment, especially after lumpectomy. Its effectiveness, however, is hindered by tumor resistance, which causes recurrence and poorer outcomes. In the last decade, nanoparticle-mediated radiosen...
A Split sfGFP-Based Signal-Amplified Reporter System for Enhanced Detection of Promoter Activity [0.03%]
一种分裂sfGFP-based信号放大报告系统用于增强启动子活性检测
Shuo Lin,Hui Yuan,Ziyi Lin et al.
Shuo Lin et al.
Fluorescent reporter systems for protein expression enable real-time, dynamic, and single-cell visualization of gene expression in living cells, but lack sufficient sensitivity for detecting low-abundance proteins. Herein, we developed a si...
Harnessing Nature's Algorithm: From Test Tubes to Autonomous In Vivo Evolution [0.03%]
利用自然算法：从试管到自主体内进化
Wenna Shang,Zhengbing Lyu,Guodong Chen
Wenna Shang
Directed evolution (DE) enables the engineering of biomolecules without prior structural knowledge. However, traditional step-wise DE is constrained by limited screening throughput. To more efficiently navigate epistatic fitness landscapes,...
Oral Lactobacillus reuteri Combined With Intravenous Lysis-Circuit-Bearing Engineered Bacteria RE Enhances Antitumor Efficacy [0.03%]
口服乳酸菌Lactobacillus reuteri结合静脉注射携带溶瘤裂解功能的工程细菌RE增强抗肿瘤疗效
Feng Geng,Lijun Yue,Maoru Du et al.
Feng Geng et al.
Engineered bacteria can preferentially colonize hypoxic tumors, but improving efficacy while limiting systemic bacterial burden remains a central challenge. Here, we combined oral Lactobacillus reuteri (LR) with intravenous administration o...
Integrated Fermentation Engineering Enables High-Level Leghemoglobin Production in Kluyveromyces marxianus via Metabolic Rewiring of Central Carbon Metabolism and Amino Acid Utilization [0.03%]
整合发酵工程通过重新布中央碳代谢和氨基酸利用路线在马克思克鲁维酵母中高效生产豆血红蛋白
Hong Chen,Xinyu Jiang,Anxiang Chen et al.
Hong Chen et al.
Leghemoglobin (LBA), a hemoprotein critical for food industry applications, faces production challenges in microbial systems due to inefficient heme-globin assembly and metabolic constraints. Here, the food-safe yeast Kluyveromyces marxianu...
Regression Models of Viability and Apoptosis Can be Generated Leveraging Cluster Analysis From Ovizio iLineF PRO Image Data [0.03%]
基于Ovizio iLineF PRO图像数据的聚类分析可以建立细胞活力和凋亡的回归模型
Joseph Fantuzzo,Nicholas Zill,John Bowers et al.
Joseph Fantuzzo et al.
Chinese hamster ovary (CHO) cells serve as the backbone of modern large-scale manufacturing of monoclonal antibodies. Central to this process are fed-batch cultures, where cells are grown from low to high cell densities and go through a mAb...
Engineering Yarrowia Lipolytica for De Novo Biosynthesis of Abscisic Acid [0.03%]
工程技术黄假丝酵母从头生物合成脱落酸
Zhengxuan Shao,Qihang Chen,Wenbao Zhao et al.
Zhengxuan Shao et al.
Abscisic acid (ABA) is a sesquiterpenoid plant hormone widely used in agriculture and the food industry. In Yarrowia lipolytica, ABA production relies on complex heterologous enzymatic pathways that have not been systematically engineered, ...
Establishment and Comparison of High-Throughput Immunoassays for Quantifying Clostridioides difficile Infection Biomarkers [0.03%]
高通量免疫测定法定量难辨梭菌感染生物标志物的建立与比较
Hanool Yun,Na-Hyeon Kwon,Minhee Kang et al.
Hanool Yun et al.
Clostridioides difficile infection (CDI) is the leading cause of healthcare-associated diarrhea, most often occurring after antibiotic exposure. In this study, we established three immunoassays: indirect enzyme-linked immunosorbent assay (E...
Comparative Study
Biotechnology journal. 2026 May;21(5):e70238. DOI:10.1002/biot.70238 2026
Engineering a Membrane-Anchored CreER Recombinase With Reduced Basal Activity for In Vitro Recombination [0.03%]
一种工程化膜定位的CreER重组酶及其在体外重组中的应用
Xiaotong Zeng,Xinyi Chen,Zishan Liang et al.
Xiaotong Zeng et al.
The CreER-loxP system is widely utilized for genetic cell lineage tracing and conditional gene function analysis. However, issues have arisen concerning the unintended labeling of nontarget cell populations, which complicate experimental da...
Dual-Similarity Driven Just-in-Time Modeling Enables Precise Real-Time Monitoring of Cell Cultures via Dielectric Spectroscopy [0.03%]
双相似性驱动的即时建模可通过介电光谱精确实时监测细胞培养物
Dongliang Chen,Qiongqiong Zhang,Zhe Lang et al.
Dongliang Chen et al.
The biopharmaceutical industry is increasingly focused on shortening development timelines to accelerate progression into clinical stages, thereby improving patient outcomes and boosting market competitiveness. Dielectric spectroscopy, a pr...