Generation of enzymatically competent SARS-CoV-2 decoy receptor ACE2-Fc in glycoengineered Nicotiana benthamiana [0.03%]
利用糖工程烟草弯胞菌生成具有酶活性的SARS-CoV-2诱饵受体ACE2-Fc
Alexandra Castilho,Jennifer Schwestka,Nikolaus F Kienzl et al.
Alexandra Castilho et al.
Human angiotensin-converting enzyme 2 (ACE2) is the primary host cell receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) binding and cell entry. Administration of high concentrations of soluble ACE2 can be utilized as...
Dimethyloxalylglycine, a small molecule, synergistically increases the homing and angiogenic properties of human mesenchymal stromal cells when cultured as 3D spheroids [0.03%]
二甲基草酰基甘氨酸通过协同作用使三维培养的人间充质干细胞的归巢和促血管生成性能增强
Marta H G Costa,Joana Serra,Todd C McDevitt et al.
Marta H G Costa et al.
Strategies aiming at increasing the survival and paracrine activity of human mesenchymal stromal cells (MSCs) are of utmost importance to achieve the full therapeutic potential of these cells. Herein, we propose both physical and biochemica...
Novel erythropoietin-based therapeutic candidates with extra N-glycan sites that block hematopoiesis but preserve neuroplasticity [0.03%]
新型促红细胞生成素疗法候选药物可阻断造血但保留神经可塑性并带有额外N-糖位点
Milagros Bürgi,Gabriela Inés Aparicio,Aquiles Dorella et al.
Milagros Bürgi et al.
Neurological disorders affect millions of people causing behavior-cognitive disabilities. Nowadays they have no effective treatment. Human erythropoietin (hEPO) has been clinically used because of its neurotrophic and cytoprotective propert...
Mixing and oxygen transfer characteristics of a microplate bioreactor with surface-attached microposts [0.03%]
表面附着有微柱的微孔板生物反应器的混合与传氧特性
Justin T Fisher,Travis O Gurney,Brittany M Mason et al.
Justin T Fisher et al.
Bioprocess optimization for cell-based therapies is a resource heavy activity. To reduce the associated cost and time, process development may be carried out in small volume systems, with the caveat that such systems be predictive for proce...
A Novel Wick-Like Paper-Based Microfluidic Device for 3D Cell Culture and Anti-Cancer Drugs Screening [0.03%]
一种新型的用于三维细胞培养和抗癌药物筛选的类似织物的纸基微流控器件
Shu-Xia Fu,Peng Zuo,Bang-Ce Ye
Shu-Xia Fu
Paper is increasingly recognized as a portable substrate for cell culture, due to its low-cost, flexible, and special porous property, which provides a native cellular 3D microenvironment. Therefore, paper-based microfluidics has been devel...
Jingdi Pan,Zongqiang Cui
Jingdi Pan
Vaccination is successfully advanced to control several fatal diseases and improve human life expectancy. However, additional innovations are required in this field because there are no effective vaccines to prevent some infectious diseases...
Oligonucleotide abundance biases aid design of a type IIS synthetic genomics framework with plant virome capacity [0.03%]
寡核苷酸丰度偏差有助于设计具有植物病毒组容量的Type IIS合成基因组框架
Fabio Pasin
Fabio Pasin
Synthetic genomics-driven dematerialization of genetic resources facilitates flexible hypothesis testing and rapid product development. Biological sequences have compositional biases, which, I reasoned, could be exploited for engineering of...
A deep learning approach to evaluate the feasibility of enzymatic reactions generated by retrobiosynthesis [0.03%]
一种深度学习方法评估由逆向生物合成生成的酶促反应的可能性
Yeji Kim,Jae Yong Ryu,Hyun Uk Kim et al.
Yeji Kim et al.
Retrobiosynthesis allows the designing of novel biosynthetic pathways for the production of chemicals and materials through metabolic engineering, but generates a large number of reactions beyond the experimental feasibility. Thus, an effec...
CRISPR/Cas12a-mediated CHO genome engineering can be effectively integrated at multiple stages of the cell line generation process for bioproduction [0.03%]
基于CRISPR/Cas12a的基因编辑技术可有效整合于CHO细胞系生物制药生产工艺的多个阶段
Patrick G Schweickert,Ning Wang,Stephanie L Sandefur et al.
Patrick G Schweickert et al.
Most biopharmaceuticals produced today are generated using Chinese hamster ovary (CHO) cells, therefore significant attention is focused on methods to improve CHO cell productivity and product quality. The discovery of gene-editing tools, s...
Engineering a thermostable chondroitinase for production of specifically distributed low-molecular-weight chondroitin sulfate [0.03%]
工程化温标糖胺聚糖裂解酶构建高效低分子量硫酸皮肤素生产体系
Hao Wang,Lin Zhang,Yang Wang et al.
Hao Wang et al.
Chondroitinase ABC I (csABC I) has attracted intensive attention because of its great potential in heparin refining and the enzymatic preparation of low-molecular-weight chondroitin sulfate (LMW-CS). However, low thermal resistance (