Molecular Mechanisms of Cellulase Biosynthesis in Trichoderma reesei: Regulatory Networks, Secretion Pathways, and Environmental Modulation [0.03%]
解码木酶产生菌曲霉中的纤维素酶生物合成分子机制：调控网络、分泌途径及环境因素的调变作用
Shibin Cui,Qinghao Wei,Mohd Sadeeq et al.
Shibin Cui et al.
Trichoderma reesei (T. reesei), a filamentous fungus, has emerged as a pivotal organism in the realm of cellulase production, garnering significant attention from researchers due to its exceptional cellulolytic properties and the potential ...
The Impact of Collection Protocol on the Yield and Purity of Mesenchymal Stem Cell-Derived Extracellular Vesicles Isolated From Serum-Free Media [0.03%]
无血清培养基中诱导多能干细胞来源的细胞外囊泡的收集方法对其得率和纯度的影响研究
Jolene Phelps,Sara Hassanpour Tamrin,Neil A Duncan et al.
Jolene Phelps et al.
The immunomodulatory and regenerative effects of mesenchymal stem cell (MSC) extracellular vesicles (EVs) have spurred the development of strategies to manufacture these EVs as therapeutics. Clinical-grade EV manufacturing requires defined ...
Biochemical and Functional Characterization of a Novel GH46 Chitosanase for Efficient Chitooligosaccharide Synthesis [0.03%]
一种高效合成壳寡糖的新型GH46型透明质酸酶的生化和功能特性研究
Mingxuan Wang,Ziyun Fang,Honglin Li et al.
Mingxuan Wang et al.
Chitosan, a derivative of the abundant biopolymer chitin, holds significant biotechnological potential but is limited by its poor solubility. Its oligomeric forms, chitooligosaccharides (COSs), exhibit superior bioactivity and solubility, d...
LaeA Orchestrates Iron-Heme Supply and P450 Catalytic Efficiency for Enhanced Echinocandin B Biosynthesis in Aspergillus nidulans [0.03%]
LaeA调控铁和血红素的供应以及P450的催化效率以增强粗糙球孢子菌中棘白菌素B的生物合成
Xiaozhang Yang,Jie Gao,Dandan Shen et al.
Xiaozhang Yang et al.
Echinocandin B (ECB), a fungal non-ribosomal lipopeptide, serves as the exclusive natural precursor of the front-line antifungal anidulafungin. Despite its clinical importance, ECB production remains suboptimal due to incomplete understandi...
Emerging Bioengineering Technologies in Female Reproduction: Preclinical Advances, Translational Challenges, and Future Outlook [0.03%]
女性生殖的新兴生物工程技术研发：临床前进展、转化挑战和未来前景展望
Thu Hang Nguyen,Hung Anh Nguyen,Y-Van Tran Thi et al.
Thu Hang Nguyen et al.
Female infertility, which affects millions of couples globally, has been a highly focused research field owing to the importance of reproduction in humans. Emerging bioengineering technologies, including tissue engineering, microfluidic chi...
Multi-Enzyme Cascade Reaction of Crude Enzyme Strategy for the Economical and Efficient Bioconversion of Rebaudioside A to Rebaudioside M [0.03%]
一种粗酶多酶级联反应策略高效经济地将甜叶菊苷A转化为甜叶菊苷M
Yuanyuan Huang,Yuxia Ge,Min Zhang et al.
Yuanyuan Huang et al.
Rebaudioside M (Reb M) is a natural sweetener with high sweetness, low caloric content, and an excellent sensory profile. However, it's extremely low natural abundance in Stevia rebaudiana leaves renders conventional extraction processes co...
Metabolic and Synthetic Biology Strategies for Enhancing Single-Cell Protein Production in Saccharomyces cerevisiae [0.03%]
用于增强酿酒酵母单细胞蛋白生产的代谢和合成生物策略
Xiaoyu Wang,Huayou Chen,Huiqing Chen et al.
Xiaoyu Wang et al.
Single cell protein (SCP), distinguished by its high production efficiency, flexible substrate utilization, and excellent nutritional value, has broad application prospects in both feed and food sectors. With the advancement of synthetic bi...
Severe Restriction of Glucose Import Enhances Recombinant Protein Production in Escherichia coli [0.03%]
严重限制葡萄糖进口可增强大肠杆菌中的重组蛋白生产
Juan Carlos Fragoso-Jiménez,Juan Carlos Sigala,Frank Delvigne et al.
Juan Carlos Fragoso-Jiménez et al.
Glucose uptake through the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) enables rapid growth of Escherichia coli but promotes overflow metabolism that limits recombinant protein production. To define how glucose transpor...
Butyrate-Producing Bacteria in Intestinal Disease Therapy: Potential and Challenges [0.03%]
肠道疾病治疗中的丁酸盐产生菌：潜力与挑战
Fenfen Zhang,Wei Zhang,Xiaoting Ren et al.
Fenfen Zhang et al.
Butyrate-producing bacteria have emerged as keystone species whose metabolic activity orchestrates host-microbial homeostasis in the human gut. This review synthesizes current understanding of how these anaerobic Firmicutes, including Faeca...
Osmolality-Independent Impact of Sodium on Glycosylation of an Fc-Fusion Protein and the Hexosamine Biosynthesis Pathway in a Chinese Hamster Ovary Cell Line [0.03%]
钠对CHO细胞中Fc融合蛋白岩藻糖基化和己糖胺 biosynthesis pathway的影响独立于渗透压
Jason Reiniger,Adrian Pöttner,Lara Rosenberger et al.
Jason Reiniger et al.
This study investigates the osmolality-independent effects of sodium and potassium on the glycosylation of an Fc-fusion protein and the activity of the hexosamine biosynthesis pathway in a Chinese Hamster Ovary cell line. Previous research ...