Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition [0.03%]
蛋白质-DNA相互作用界定了IS2转座中环形成和插入反应的机制方面
Leslie A Lewis,Mekbib Astatke,Peter T Umekubo et al.
Leslie A Lewis et al.
Background: Transposition in IS3, IS30, IS21 and IS256 insertion sequence (IS) families utilizes an unconventional two-step pathway. A figure-of-eight intermediate in Step I, from asymmetric single-strand cleavage and joi...
Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis [0.03%]
异源四膜虫Sleeping Beauty转座子在Xenopus tropicalis生殖系中的活化
Donald A Yergeau,Clair M Kelley,Emin Kuliyev et al.
Donald A Yergeau et al.
Background: The Sleeping Beauty (SB) transposon system has been used for germline transgenesis of the diploid frog, Xenopus tropicalis. Injecting one-cell embryos with plasmid DNA harboring an SB transposon substrate toge...
Soluble expression, purification and characterization of the full length IS2 Transposase [0.03%]
IS2转座酶全长可溶性表达、纯化及性质表征
Leslie A Lewis,Mekbib Astatke,Peter T Umekubo et al.
Leslie A Lewis et al.
Background: The two-step transposition pathway of insertion sequences of the IS3 family, and several other families, involves first the formation of a branched figure-of-eight (F-8) structure by an asymmetric single stran...
Characterization and potential functional significance of human-chimpanzee large INDEL variation [0.03%]
人类和黑猩猩大片段插入/删除变异的特征及潜在的功能意义
Nalini Polavarapu,Gaurav Arora,Vinay K Mittal et al.
Nalini Polavarapu et al.
Background: Although humans and chimpanzees have accumulated significant differences in a number of phenotypic traits since diverging from a common ancestor about six million years ago, their genomes are more than 98.5% i...
Crypton transposons: identification of new diverse families and ancient domestication events [0.03%]
加密子转座子：新家族的识别及古老驯化事件的鉴定
Kenji K Kojima,Jerzy Jurka
Kenji K Kojima
Background: "Domestication" of transposable elements (TEs) led to evolutionary breakthroughs such as the origin of telomerase and the vertebrate adaptive immune system. These breakthroughs were accomplished by the adaptat...
Retrotransposition of R2 elements in somatic nuclei during the early development of Drosophila [0.03%]
R2元件在果蝇早期发育过程中体细胞核中的逆转座事件
Michael T Eickbush,Thomas H Eickbush
Michael T Eickbush
Background: R2 retrotransposable elements exclusively insert in the 28S rRNA genes of their host. Their RNA transcripts are produced by self-processing from a 28S R2 cotranscript. Because full-length R2 transcripts are fo...
George W Cook,Miriam K Konkel,James D Major rd et al.
George W Cook et al.
Background: The human genome contains approximately one million Alu elements which comprise more than 10% of human DNA by mass. Alu elements possess direction, and are distributed almost equally in positive and negative s...
DNA binding activities of the Herves transposase from the mosquito Anopheles gambiae [0.03%]
来源于按蚊的Herves转座酶的DNA结合活性
Amandeep S Kahlon,Robert H Hice,David A O&#x;Brochta et al.
Amandeep S Kahlon et al.
Background: Determining the mechanisms by which transposable elements move within a genome increases our understanding of how they can shape genome evolution. Class 2 transposable elements transpose via a 'cut-and-paste' ...
Mobile DNA and the TE-Thrust hypothesis: supporting evidence from the primates [0.03%]
移动DNA和转座推动力假说：来自灵长类动物的支持证据
Keith R Oliver,Wayne K Greene
Keith R Oliver
Transposable elements (TEs) are increasingly being recognized as powerful facilitators of evolution. We propose the TE-Thrust hypothesis to encompass TE-facilitated processes by which genomes self-engineer coding, regulatory, karyotypic or ...
Jens Mayer,Jonas Blomberg,Ruth L Seal
Jens Mayer
Background: Endogenous retroviruses (ERVs) and ERV-like sequences comprise 8% of the human genome. A hitherto unknown proportion of ERV loci are transcribed and thus contribute to the human transcriptome. A small proporti...