Quantitative impedance-based characterization of breast cancer cell migration and metastatic potential [0.03%]
基于阻抗的定量表征乳腺癌细胞迁移和转移潜能
Kyndra S Higgins,Cheryl T Gomillion
Kyndra S Higgins
Background: Cellular impedance-based assays offer a sensitive, label-free, and non-destructive method to continuously monitor cells in real time, allowing the assessment of both kinetics and degree of migration for breast...
Insect cell expression system: advances in applications, engineering strategies, and bioprocess development [0.03%]
昆虫细胞表达系统：应用、工程策略及生物工艺开发方面的新进展
Nongyu Huang,Yuquan Wei,Jiong Li
Nongyu Huang
As technology and regulatory guidelines continue improve, insect cell expression system has gained increasing attention as promising and versatile platforms for production of biopharmaceuticals. This review provides a comprehensive overview...
Sustainable valorization of fish head wastes into nitrogen-based nutrients: a comparative life cycle assessment [0.03%]
基于氮素的鱼类 heads 可持续高值化利用：生命周期评估比较研究
Somyong Lee,Soeon Ahn,Jin Ho Zong et al.
Somyong Lee et al.
The production of nitrogen-based nutrients is a major contributor to climate change, with industrial ammonia synthesis releasing over 500 million tonnes of CO₂-equivalents annually—more than 1.2% of global anthropogenic greenhouse gas (GH...
Bioengineered injectable HAMA/GelMA hydrogel encapsulating exosomes loaded lycopene mitigates deoxynivalenol-induced testicular injury via apoptotic pathway modulation [0.03%]
生物工程可注射HAMA /凝胶封装装载番茄红素的外泌体通过调节凋亡途径来缓解脱氧雪腐镰刀菌烯醇诱导的睾丸损伤
Feng Ru,Ramaiyan Velmurugan,Chunfeng Li et al.
Feng Ru et al.
Deoxynivalenol (Dex), a widespread mycotoxin found in contaminated cereals, induces testicular dysfunction primarily through oxidative stress, inflammation, and activation of apoptotic pathways. Lycopene (Lyc), a natural antioxidant, offers...
Cinnamaldehyde/cinnamon essential oil loaded poly-L-lactic acid/ hydroxyapatite fibrous scaffolds as osteogenic differentiation enhancing system for bone tissue engineering applications [0.03%]
负载肉桂醛/肉桂精油的聚乳酸/羟基磷灰石纤维支架促进骨组织工程中成骨分化作用
Fateme Darchin Tabrizi,Mehdi Doosti-Telgerd,Narges Forouzideh et al.
Fateme Darchin Tabrizi et al.
CRISPR/Cas9-mediated mutation of GhCAD decreases the gossypol content of cottonseed [0.03%]
CRISPR/Cas9介导的GhCAD突变降低棉籽中的古柯碱含量
Lili Zhou,Yali Wang,Jiamin Wang et al.
Lili Zhou et al.
Cottonseed is the most important byproduct of cotton production. However, high free gossypol contents limit the application of cottonseed in the food or feed industry. In this study, CRISPR/Cas9 technology was used to knock out the (+)-δ-c...
Streamlined purification of ADDomer nanoparticles for scalable biomanufacturing [0.03%]
用于可扩展生物制造的ADDomer纳米粒子的精简纯化方法
Raquel Arinto-Garcia,Cláudia S Paiva,Emerson Bryan Dilla et al.
Raquel Arinto-Garcia et al.
Current lab-scale purification methods for ADDomer are labour-intensive, time-consuming and poorly scalable. The intracellular nature of ADDomer production further complicates downstream processing, requiring robust, scalable solutions for ...
Combinatorial engineering pinpoints shikimate pathway bottlenecks in para-aminobenzoic acid production in Pseudomonas putida [0.03%]
组合工程在Pseudomonas putida中定位了对氨基苯甲酸生物合成途径的限速步骤
Marco A Campos-Magaña,Sara Moreno-Paz,Maria Martin-Pascual et al.
Marco A Campos-Magaña et al.
Combinatorial expression libraries to optimize multigene pathways can improve product titers, but the large number of potential genetic variants makes exhaustive testing impractical. Statistical Design of Experiments (DoE) offers a powerful...
Systemic versus local delivery of mesenchymal stem cells to improve the early stages of fracture healing in a polytrauma model [0.03%]
系统给药和局部给药间充质干细胞以改善多发伤模型骨折早期愈合状况
Augustine Mark Saiz,Maryam Rahmati,Soren David Johnson et al.
Augustine Mark Saiz et al.
Fracture healing complications, including nonunion, are common in polytrauma patients partly due to systemic inflammatory dysregulation. Although mesenchymal stem cells (MSCs) have been widely explored for their regenerative properties, the...
Efficient site-specific recombination by self-activating split-Dre recombinase in mammalian cells and E. coli [0.03%]
利用自激活的裂解 Dre 重组酶在哺乳动物细胞和大肠杆菌中进行高效特异性位点重排
Chichu Xie,Jinfeng Gan,Yuanhao Zhang et al.
Chichu Xie et al.
Background: Site-specific recombination (SSR) systems are essential tools for conditional genetic manipulation and are valued for their efficacy and user friendliness. However, the development of novel SSR strategies is u...