An optimized CRISPR/Cas9-based genome editing platform enhances bacteriocin production in Lactiplantibacillus plantarum [0.03%]
一种优化的CRISPR/Cas9基因编辑平台可增强植物乳杆菌中乳酸素的产生
Rajat Anand,Rudolf Lütticken,Laura De Laporte et al.
Rajat Anand et al.
Efficient genetic engineering of lactic acid bacteria remains technically challenging due to their thick peptidoglycan cell wall, low transformation efficiency, strain-specific restriction-modification systems, and sensitivity to Cas9-induc...
A versatile yeast platform for N-demethylation of therapeutic alkaloids [0.03%]
一种用于治疗用生物碱N-去甲基化的酵母通用平台
Daniel J Yaw,Xu Li,Katrine B Kampmann et al.
Daniel J Yaw et al.
Alkaloid N-demethylation is a critical step in the production of many semi-synthetic alkaloid therapeutics and has traditionally relied on synthetic chemical routes. Microbial whole-cell bioproduction offers a promising alternate route for ...
A signal peptide-guided approach towards in situ functionalization of bacterial nanocellulose in Komagataeibacter rhaeticus [0.03%]
一种信号肽引导的方法：在葡萄糖丁酸醋杆菌中实现纳米纤维素的原位功能化
Jenni Vannas,Amritpal Singh,Bibi Hannikainen et al.
Jenni Vannas et al.
Background: Bacterial nanocellulose (BC), produced by Komagataeibacter species, is an ideal scaffold for biological Engineered Living Materials (bioELMs) research. Current BC functionalization strategies often rely on sec...
Smart colorimetric wound dressing for real-time infection detection and monitoring [0.03%]
用于实时感染检测和监测的智能色度伤口敷料
Hossein Rayat Pisheh,Razieh Rezaeian,Mahsa Sani et al.
Hossein Rayat Pisheh et al.
Wound infection remains a major clinical challenge, leading to delayed healing, prolonged hospitalization, and increased healthcare costs. Conventional wound dressings provide limited protection and lack the ability to offer real-time infor...
Digital light processing bioprinting: bioink innovations and applications in tissue and organ regeneration [0.03%]
数字光处理生物打印：组织和器官再生中的生物墨水创新及应用
Hossein Rayat Pisheh,Monireh Sadat Hoseinian,Delaram Dezfoulian et al.
Hossein Rayat Pisheh et al.
Digital light processing (DLP) bioprinting offers superior spatial resolution, rapid printing speed, and gentle cell handling compared to extrusion- and inkjet-based methods. By enabling layer-by-layer photopolymerization through projected ...
An advanced genome engineering platform for Komagataella phaffii enabling versatile in vivo DNA assembly and multiplex integration [0.03%]
一种先进的基因组工程平台，可实现Komagataella phaffii中体内DNA组装和多重整合
Yujung Choi,Ayoung Kim,Nam Kyu Kang
Yujung Choi
Background: The transition toward a sustainable bioeconomy necessitates the development of robust microbial platforms capable of C1 chemical valorization, particularly the conversion of methanol into high-value chemicals....
A strategy of 3D-printed Ta4C3 MXenes nanohydroxyapatite/methacryloylated silk fibroin scaffolds for postsurgical management of osteosarcoma [0.03%]
用于骨肉瘤术后管理的3D打印二硼化钛纳米羟基磷灰石/甲基丙烯酸修饰丝素蛋白支架策略
Liu Yang,Kenan Sun,Shihao Deng et al.
Liu Yang et al.
The postoperative repair of bone tumors remains a significant clinical challenge requiring urgent solutions, and this holds particularly true for regenerating large bone defects, completely removing residual tumor cells, and effectively pre...
A thermosensitive hydrogel delivery system incorporating wogonin-treated mesenchymal stromal cell (MSC)-derived exosomes alleviates osteoarthritis [0.03%]
装载葳甘草黄酮处理的间质干细胞源性外泌体的热敏水凝胶递送系统可缓解骨关节炎
Kewei Zhu,Minzhi Mao,Wenzhao Li et al.
Kewei Zhu et al.
Osteoarthritis (OA) is a prevalent degenerative joint disease affecting major joints, causing pain and functional impairment due to cartilage degeneration and osteophyte formation. Current treatments have significant limitations. Mesenchyma...
Rewiring a methanol-responsive regulatory system improves glucose-methanol co-utilization in Eubacterium limosum [0.03%]
在Eubacterium limosum中重塑甲醇调控系统以改善葡萄糖和甲醇的协同利用
Jiyun Bae,Hyunwoo Jung,Seulgi Kang et al.
Jiyun Bae et al.
Background: Methanol is a promising one-carbon (C1) feedstock for sustainable bioproduction, and its mixotrophic co-utilization with other substrates can improve product formation. However, mixotrophy often leads to seque...
Melatonin accelerated angiogenesis activity of alginate-gelatin encapsulated endothelial progenitor cells in a mouse model of volumetric muscle loss [0.03%]
褪黑素加速藻酸盐-明胶封装的内皮前体细胞在体积性肌肉损失小鼠模型中的血管生成活性
Somayyeh Rashidi,Çığır Biray Avci,Ghasem Bagherpour et al.
Somayyeh Rashidi et al.
Volumetric muscle loss (VML) is a challenging clinical condition, resulting in the lack of appropriate function. The induction of angiomyogenesis can, in part, restore the function of injured sites using tissue engineering and stem cells. I...