Synthetic redesign of Corynebacterium glutamicum for 1,4-butanediol production via L-glutamate-derived CoA-independent pathway [0.03%]
基于谷氨酸的辅酶独立合成途径使棒状杆菌谷氨亚胺成为1,4-丁二醇生产菌株
Jinyoung Kim,Hyeon Jin Kim,Sukjae Han et al.
Jinyoung Kim et al.
1,4-Butanediol (1,4-BDO) is a key commodity chemical used in the production of various products, including plastics, fibers, and polyesters. Here, a potent microbial platform for 1,4-BDO production from glucose via the L-glutamate-derived C...
A synthetic metabolic pathway for the de novo biosynthesis of medium chain length γ- and δ-lactones [0.03%]
从头合成中链长度γ-和δ-内酯的新代谢途径的合成
Jonathan Muller,Angel Xia,Patrik Jones
Jonathan Muller
Background: Medium chain length γ- and δ-lactones, in particular γ-decalactone, are potent aroma compounds used by the flavour and fragrance industry. The native pathways for their biosynthesis are unknown so to facili...
The construction of the gelatin methacrylate microneedle -encapsulated newcastle disease virus and its antitumor effect in hepatocellular carcinoma [0.03%]
明胶甲基丙烯酸微针递送新城疫病毒的抗肿瘤效应研究
Tong Li,Xiyu Liu,Jintong Na et al.
Tong Li et al.
Background: Hepatocellular carcinoma (HCC) is associated with one of the highest cancer-related mortality rates worldwide and remains therapeutically challenging. There is an urgent need for innovative treatment strategie...
Integration of TLR7/8 agonists into lipid nanoparticles enhances antigen-specific immune responses to N1-methyl-Ψ-modified mRNA-LNP vaccines [0.03%]
TLR7/8激动剂与脂质纳米颗粒整合可增强N1-甲基-Ψ修饰型mRNA-LNP疫苗的抗原特异性免疫反应
Huijeong Choi,Seonghyun Lee,Hyejin Kim et al.
Huijeong Choi et al.
N1-methylpseudouridine (N1-methyl-Ψ)-modified mRNA offers a safer alternative to unmodified mRNA-based cancer immunotherapies but induces weaker innate immune responses. This study aimed to enhance the expression of N1-methyl-Ψ-modified m...
Nicholaus L DeCuzzi,Jason Y Hu,Florene Xu et al.
Nicholaus L DeCuzzi et al.
Cell fate decisions are regulated by intricate signaling networks, with Extracellular signal-Regulated Kinase (ERK) being a central node in the control of cell proliferation and differentiation. ERK typically cooperates with a network of ot...
Efflux pump knockout minimizes crosstalk and boosts response in transcription factor-based biosensors [0.03%]
效应泵敲除可减少交叉-talk并增强基于转录因子的生物传感器的响应能力
Jinju Lee,Byeong-Hyeon Yoo,Eunjung Jeon et al.
Jinju Lee et al.
Background: Cellular transport systems are key determinants of intracellular molecule concentrations and can be utilized to modulate the responsiveness of transcription factor (TF)-based biosensors. Efflux pumps facilitat...
FTO-engineered extracellular vesicles from bone marrow mesenchymal stem cells ameliorate Staphylococcus aureus-induced osteomyelitis via m6A-dependent suppression of autophagy and pyroptosis [0.03%]
脂肪细胞工程化骨髓间充质干细胞来源的外泌体通过m6A依赖抑制自噬和焦亡改善金黄色葡萄球菌诱导的骨髓炎
Muguo Song,Siyu Lu,Hao Tang et al.
Muguo Song et al.
Background: Bone marrow mesenchymal stem cells (BM-MSCs) ameliorate various orthopedic diseases through the secretion of extracellular vesicles (EVs). This study investigates the effects of FTO-overexpressing BM-MSCs-deri...
Thrombomodulin is essential for recombinant Fc-fusion protein production in Chinese hamster ovary cells via multiple signaling pathways [0.03%]
血栓调节蛋白通过多种信号通路在中国仓鼠卵巢细胞中分泌型重组融合蛋白的生产中起关键作用
Xiaonan Ma,Nawei Wang,Qihang Yan et al.
Xiaonan Ma et al.
Background: Chinese hamster ovary (CHO) cells are the predominant cell line used for biotherapeutic production. To reduce the cost of therapeutic recombinant proteins produced in CHO cells, efforts have been made over dec...
Human preclinical multiple myeloma in vitro models for disease modeling and therapy screening [0.03%]
用于疾病建模和药物筛选的人类多发性骨髓瘤体外模型
Mikayla Ybarra,Jaehun Lee,Yun-Ya Chen et al.
Mikayla Ybarra et al.
Multiple myeloma (MM) is a hematologic malignancy characterized by uncontrolled expansion of malignant plasma cells within the bone marrow microenvironment. While suspension cultures of MM cell lines and murine models have been the cornerst...
Biomaterials for fetal membrane repair in preterm premature rupture of membranes: advances in tissue engineering strategies [0.03%]
用于未足月胎膜早破的胎儿膜修复的生物材料：组织工程策略方面的进展
Limei Fan,Zongyu Liu,Xuesong Li et al.
Limei Fan et al.
Preterm premature rupture of membranes (PPROM) remains a leading cause of spontaneous preterm birth and neonatal morbidity, yet current clinical management strategies are limited. The fetal membranes, composed of the amnion and chorion, pos...