New insights into hydroxyectoine synthesis and its transcriptional regulation in the broad-salt growing halophilic bacterium Chromohalobacter salexigens [0.03%]
广盐性的嗜盐杆菌Chromohalobacter salexigens中羟基乙内酰脲合成及转录调控的新见解
Montserrat Argandoña,Francine Piubeli,Mercedes Reina-Bueno et al.
Montserrat Argandoña et al.
Elucidating the mechanisms controlling the synthesis of hydroxyectoine is important to design novel genetic engineering strategies for optimizing the production of this biotechnologically relevant compatible solute. The genome of the haloph...
Repurposing the FDA-approved anticancer agent ponatinib as a fluconazole potentiator by suppression of multidrug efflux and Pma1 expression in a broad spectrum of yeast species [0.03%]
一种抗肿瘤药物帕纳替尼通过抑制多药外排和Pma1表达再利用为一系列酵母菌广谱氟康唑增效剂
Lin Liu,Tong Jiang,Jia Zhou et al.
Lin Liu et al.
Fungal infections have emerged as a major global threat to human health because of the increasing incidence and mortality rates every year. The emergence of drug resistance and limited arsenal of antifungal agents further aggravates the cur...
Redox-sensitive transcriptional regulator SoxR directly controls antibiotic production, development and thiol-oxidative stress response in Streptomyces avermitilis [0.03%]
链霉菌素红氧化还原敏感型转录调节因子SoxR直接控制安伟利霉素产生、发育和巯基氧化应激响应
Qian Wang,Xiaorui Lu,Haixin Yang et al.
Qian Wang et al.
The redox-sensitive transcriptional regulator SoxR is conserved in bacteria. Its role in mediating protective response to various oxidative stresses in Escherichia coli and related enteric bacteria has been well established. However, functi...
Flexible integrated sensing platform for monitoring wound temperature and predicting infection [0.03%]
伤口温度监测和感染预测的柔性集成传感平台
Yuheng Zhang,Bin Lin,Rong Huang et al.
Yuheng Zhang et al.
Wound infection is a challenging clinical problem that imposes substantial economic and psychological burdens on patients. However, the wound covered by a dressing is in an 'unknown' state. Recently, researchers have focused on understandin...
A multi-functional genetic manipulation system and its use in high-level expression of a β-mannanase mutant with high specific activity in Pichia pastoris [0.03%]
一种多功能遗传操作系统及其在毕赤酵母中高效表达高比活木聚糖酶突变体中的应用
Zhemin Liu,Linyuan Cao,Xiaodan Fu et al.
Zhemin Liu et al.
To further extend the practical application of a thermostable and acidic resistance β-mannanase (ManAK) in animal feed additives, an effective strategy that combined directed evolution and metabolic engineering was developed. Four positive...
Metabolic regulation adapting to high methanol environment in the methylotrophic yeast Ogataea methanolica [0.03%]
甲基营养型酵母Ogataea methanolica适应高浓度甲醇环境的代谢调控研究
Hao-Liang Cai,Ryohei Doi,Masaya Shimada et al.
Hao-Liang Cai et al.
Since methylotrophic yeasts such as Ogataea methanolica can use methanol as a sole carbon feedstock, they could be applied to produce valuable products from methanol, a next-generation energy source synthesized from natural gases, using gen...
Molecular analysis of the replication functions of the bifidobacterial conjugative megaplasmid pMP7017 [0.03%]
毕赤氏杆菌接合型巨质粒pMP7017复制功能的分子分析研究
Rebecca L Dineen,Christophe Penno,Philip Kelleher et al.
Rebecca L Dineen et al.
pMP7017 is a conjugative megaplasmid isolated from the gut commensal Bifidobacterium breve JCM7017 and was shown to encode two putative replicases, designated here as RepA and RepB. In the current work, RepB was identified as the pMP7017 re...
A multiplex antigen microarray for simultaneous IgG and IgM detection against SARS-CoV-2 reveals higher seroprevalence than reported [0.03%]
一种用于同时检测SARS-CoV-2的IgG和IgM的多重抗原微阵列芯片显示比以往报道更高的血清阳性率
David Ruano-Gallego,Miriam García-Villadangos,Mercedes Moreno-Paz et al.
David Ruano-Gallego et al.
The surge of SARS-CoV-2 has challenged health systems worldwide and efficient tests to detect viral particles, as well as antibodies generated against them, are needed. Specificity, sensitivity, promptness or scalability are the main parame...
Sustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi-carbon utilizing pathway in obligate methanotrophic bacteria [0.03%]
通过重建严格甲基营养细菌中的多碳利用途径实现甲烷和甘油的可持续生物转化制备化学品
Hoa Thi Quynh Le,Anh Duc Nguyen,Ye Rim Park et al.
Hoa Thi Quynh Le et al.
Obligate methanotrophic bacteria can utilize methane, an inexpensive carbon feedstock, as a sole energy and carbon substrate, thus are considered as the only nature-provided biocatalyst for sustainable biomanufacturing of fuels and chemical...