The Impact of Methanol Concentration on Recombinant Protein Glycosylation in Pichia pastoris SuperMan5 [0.03%]
Andre Ohara,Zhang Pengyue,Xinqi Cao et al.
Andre Ohara et al.
The methylotrophic yeast Pichia pastoris (also known as Komagataella phaffii) is a prominent platform for recombinant protein production, offering benefits such as thermo- and osmotolerance, high-density growth, and efficient protein secret...
Multi-Halogenated Indoles as Antimicrobial and Antivirulence Agents Against Drug-Resistant Staphylococcus aureus [0.03%]
具有抗微生物和抗毒力作用的多卤代色原酮对抗药性金黄色葡萄球菌的作用研究
MinHwi Sim,Bharath Reddy Boya,Yong-Guy Kim et al.
MinHwi Sim et al.
The emergence of multidrug-resistant Staphylococcus aureus requires the development of novel agents that can target both planktonic cells and persistent biofilms. In this study, a library of multi-halogenated indoles was evaluated for antib...
Unveiling the Potential of Lentilactobacillus hilgardii in Malolactic Fermentation: Comparative Genomics and Fermentation Dynamics [0.03%]
解码乳酸片球菌希尔加迪氏菌在苹果酸-乳酸发酵中的潜力：比较基因组和发酵动力学研究
Giacomo Mantegazza,Nicola Mangieri,Elnaz Vojoudi Yazdi et al.
Giacomo Mantegazza et al.
This study aimed to assess the potential of Lentilactobacillus hilgardii as a novel candidate for malolactic fermentation (MLF) in winemaking, through comparative genomics and experimental validation, in direct comparison with Oenococcus oe...
Comparative Study
Microbial biotechnology. 2025 Dec;18(12):e70259. DOI:10.1111/1751-7915.70259 2025
Metagenomic Insights Into the Ecology, Taxonomy and Metabolic Capabilities of 'Candidatus Darwinibacteriales' Ord. Nov. (Formerly MBA03), a Potential Key Player in Anaerobic Digestion [0.03%]
宏基因组学揭示了“Darwinibacteriales”新目（原MBA03）的生态、分类和代谢能力，可能是在厌氧消化过程中发挥关键作用的重要因素
Roser Puchol-Royo,Javier Pascual,Asier Ortega-Legarreta et al.
Roser Puchol-Royo et al.
Biogas, a mix of CO2, CH4 and small proportions of other gases, is a biofuel obtained by anaerobic digestion (AD). Biogas production is often considered a black box process, as the role and dynamics of some of the microorganisms involved re...
Mining Actinomycetes' Metabolomes and Genomes for Anti-Phytophthora infestans Compounds [0.03%]
链霉菌代谢组和基因组的挖掘以寻找抗致病疫霉化合物
Ola Abdelrahman,Quinn Coxon,Eliane Abou-Mansour et al.
Ola Abdelrahman et al.
Actinomycetes are well-known for producing a diverse array of specialised metabolites with various bioactivities; yet, identifying metabolites with targeted activity against specific pathogens remains challenging. In this study, we employed...
Biocontrol of Root-Knot Nematodes via siRNA-Loaded Extracellular Vesicles From a Nematophagous Fungus Arthrobotrys oligospora [0.03%]
球孢白僵菌胞外囊泡介导的siRNA生物控刺螠根结线虫疾病研究
Xinyi Huang,Wenliang Zhou,Xiaoying Liu et al.
Xinyi Huang et al.
Root-knot nematodes (Meloidogyne spp.) represent a major threat to global crop production, and current chemical nematicides pose serious environmental and health risks. RNA interference (RNAi) offers a promising gene-specific strategy for n...
Glycosylated Foot-And-Mouth Disease Virus-Like Particles Produced in Pichia Pastoris Enhance Stability and Immunogenicity [0.03%]
在毕赤酵母中糖基化修饰的口蹄疫病毒样颗粒能够提高稳定性及免疫原性
Zhiyao Li,Hu Dong,Shuanghui Yin et al.
Zhiyao Li et al.
Despite the availability of vaccines, foot-and-mouth disease (FMD) remains a significant concern in many developing countries, causing severe economic losses and affecting local farming communities. Virus-like particle (VLP) vaccines are hi...
Antoine Danchin,Victor de Lorenzo,Pablo Iván Nikel et al.
Antoine Danchin et al.
Despite its unusual structure and detrimental role as a chaotropic guanidinium ion, guanidine [HNC(NH2)2] exists as a genuine metabolite in many microbes, and its negative effects are mitigated by specific exporters. The metabolic origin of...
Microbial Conversion of Glycerol Into 1,3-Propanediol by Fermentation: Review of Fundamentals and Operational Strategies [0.03%]
甘油发酵法转化生成1,3-丙二醇的微生物技术：基本要素及操作策略综述
María Fernanda Pérez-Bernal,Roman Moscoviz,Xiaoli Wang et al.
María Fernanda Pérez-Bernal et al.
Over the past decades, biodiesel production has sharply increased worldwide and has led to an overproduction of glycerol, as by-product. Therefore, glycerol is not only produced at low cost with a wide availability but is also a versatile p...
Centromeric Sequences in Ogataea polymorpha Genome Enable Development of Stable Multigene Expression Plasmid Tools [0.03%]
丙酸威杆菌染色体着丝粒序列的鉴定及构建多功能稳定复制表达质粒
Yanfei Cheng,Yuanyuan Shi,Ning Sun et al.
Yanfei Cheng et al.
Centromeres are the chromosomal sites at which the kinetochore forms and attaches to spindle microtubules, directing chromosome segregation. Plasmids based on centromeres can maintain stability and distribute accurately during cell division...