Chimeric Phi29 DNA polymerase with helix-hairpin-helix motifs shows enhanced salt tolerance and replication performance [0.03%]
具有螺旋-发夹-螺旋基序的嵌合Phi29 DNA聚合酶表现出增强的耐盐性和复制性能
Yaping Gao,Yun He,Liyi Chen et al.
Yaping Gao et al.
Phi29 DNA polymerase (Phi29 Pol) has been successfully applied in DNA nanoball-based sequencing, real-time DNA sequencing from single polymerase molecules and nanopore sequencing employing the sequencing by synthesis (SBS) method. Among the...
A novel toxoflavin-quenching regulation in bacteria and its application to resistance cultivars [0.03%]
细菌中一个新的诱导型毒氟磷抗性调控机制及在抗病品种中的应用
Okhee Choi,Yeyeong Lee,Jiyeong Park et al.
Okhee Choi et al.
The toxoflavin (Txn), broad host range phytotoxin produced by a variety of bacteria, including Burkholderia glumae, is a key pathogenicity factor of B. glumae in rice and field crops. Two bacteria exhibiting Txn-degrading activity were isol...
Development of dual-inducible duet-expression vectors for tunable gene expression control and CRISPR interference-based gene repression in Pseudomonas putida KT2440 [0.03%]
用于调节基因表达控制和假单胞菌KT2440中基于CRISPR干扰的基因抑制的双诱导二重表达载体的开发
Rahul Gauttam,Aindrila Mukhopadhyay,Blake A Simmons et al.
Rahul Gauttam et al.
The development of P. putida as an industrial host requires a sophisticated molecular toolbox for strain improvement, including vectors for gene expression and repression. To augment existing expression plasmids for metabolic engineering, w...
Role of phenazine-enzyme physiology for current generation in a bioelectrochemical system [0.03%]
生物电化学系统中吩嗪-酶生理在电流产生中的作用
Anthony Chukwubuikem,Carola Berger,Ahmed Mady et al.
Anthony Chukwubuikem et al.
Pseudomonas aeruginosa produces phenazine-1-carboxylic acid (PCA) and pyocyanin (PYO), which aid its anaerobic survival by mediating electron transfer to distant oxygen. These natural secondary metabolites are being explored in biotechnolog...
Genetic engineering biofilms in situ using ultrasound-mediated DNA delivery [0.03%]
利用超声波介导的DNA递送方法进行现场基因工程菌群改造
Chun Kiat Ng,Samuel L Putra,Joseph Kennerley et al.
Chun Kiat Ng et al.
The ability to directly modify native and established biofilms has enormous potential in understanding microbial ecology and application of biofilm in 'real-world' systems. However, efficient genetic transformation of established biofilms a...
Saskia von Stillfried,Sophia Villwock,Roman D Bülow et al.
Saskia von Stillfried et al.
Virus detection methods are important to cope with the SARS-CoV-2 pandemics. Apart from the lung, SARS-CoV-2 was detected in multiple organs in severe cases. Less is known on organ tropism in patients developing mild or no symptoms, and som...
COVID-19: vaccine's progress [0.03%]
新冠疫苗进展
Harald Brüssow
Harald Brüssow
Under the pressure of the COVID-19 pandemic, vaccines were developed and rolled out into mass vaccination campaigns at incredible speed. What normally takes a decade was worked out within a year. Vaccines were produced along many different ...
Programming living sensors for environment, health and biomanufacturing [0.03%]
用于环境、健康和生物制造的活体传感器编程
Xinyi Wan,Behide Saltepe,Luyang Yu et al.
Xinyi Wan et al.
Synthetic biology offers new tools and capabilities of engineering cells with desired functions for example as new biosensing platforms leveraging engineered microbes. In the last two decades, bacterial cells have been programmed to sense a...
Convergent phenotypic evolution towards fosfomycin collateral sensitivity of Pseudomonas aeruginosa antibiotic-resistant mutants [0.03%]
假单胞菌抗生素耐药突变体向fosfomycin平行敏感性演化的收敛现象
Pablo Laborda,José L Martínez,Sara Hernando-Amado
Pablo Laborda
The rise of antibiotic resistance and the reduced amount of novel antibiotics support the need of developing novel strategies to fight infections, based on improving the use of the antibiotics we already have. Collateral sensitivity is an e...
Sporulation is dispensable for the vegetable-associated life cycle of the human pathogen Bacillus cereus [0.03%]
芽孢生成对于人类病原菌蜡样芽孢杆菌的植物相关生活周期是可有可无的
María Luisa Antequera-Gómez,Luis Díaz-Martínez,Juan Antonio Guadix et al.
María Luisa Antequera-Gómez et al.
Bacillus cereus is a common food-borne pathogen that is responsible for important outbreaks of food poisoning in humans. Diseases caused by B. cereus usually exhibit two major symptoms, emetic or diarrheic, depending on the toxins produced....