Effects of dual deletion of glnR and mtrA on expression of nitrogen metabolism genes in Streptomyces venezuelae [0.03%]
glnR和mtrA双缺失对 venezuelaensis氮代谢基因表达的影响
Yanping Zhu,Jiao Wang,Wenya Su et al.
Yanping Zhu et al.
GlnR activates nitrogen metabolism genes under nitrogen-limited conditions, whereas MtrA represses these genes under nutrient-rich conditions in Streptomyces. In this study, we compared the transcription patterns of nitrogen metabolism gene...
Tools of the trade: plasmid repositories and standardized plasmid manipulation for molecular and synthetic biology [0.03%]
分子和合成生物学中的工具菌株资源库及质粒标准化操作
Stephen K Dolan,Miguel A Matilla
Stephen K Dolan
Plasmids are extrachromosomal genetic elements capable of autonomous replication within a host cell. They play a key role in bacterial ecology and evolution, facilitating the mobilization of accessory genes by horizontal gene transfer. Cruc...
Cheng-Gang Ren,Zheng-Yi Liu,Xiao-Li Wang et al.
Cheng-Gang Ren et al.
In the ocean, seaweed and microorganisms have coexisted since the earliest stages of evolution and formed an inextricable relationship. Recently, seaweed has attracted extensive attention worldwide for ecological and industrial purposes, bu...
Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re-establishment ability [0.03%]
冻干法保存人类肠道菌群：培养pH值对干燥结果和重建能力的影响
Regina Haindl,Lisa Totzauer,Ulrich Kulozik
Regina Haindl
Faecal microbiota transplantation is an emerging medical concept for the treatment of gastrointestinal diseases. This concept, however, has disadvantages as low storability of stool and intensive donor screening. A solution to overcome thes...
The essential differences in microbial and chemical components of musk of different qualities secreted by captive male forest musk deer (Moschus berezovskii) [0.03%]
不同品质人工饲养雄林麝分泌素的化学及微生物组分差异研究
Yan Wang,Mao Sun,Fan Chang et al.
Yan Wang et al.
Musk is a precious raw material and ingredient in Chinese traditional medicine. The production of unqualified musk has become a puzzling problem in forest musk deer (FMD) breeding. However, what the essential differences between so-called u...
Highly efficient rDNA-mediated multicopy integration based on the dynamic balance of rDNA in Saccharomyces cerevisiae [0.03%]
以rDNA动态平衡为基础的高效rDNA介导的多拷贝染色体整合技术
Huihui Zheng,Kai Wang,Xiaoxiao Xu et al.
Huihui Zheng et al.
Engineered Saccharomyces cerevisiae strains are good cell factories, and developing additional genetic manipulation tools will accelerate construction of metabolically engineered strains. Highly repetitive rDNA sequence is one of two main s...
Oligomerization engineering of the fluorinase enzyme leads to an active trimer that supports synthesis of fluorometabolites in vitro [0.03%]
寡聚化工程改造的含氟酶生成具有体内合成含氟代谢物活性的三聚体
Tiia Kittilä,Patricia Calero,Folmer Fredslund et al.
Tiia Kittilä et al.
The fluorinase enzyme represents the only biological mechanism capable of forming stable C-F bonds characterized in nature thus far, offering a biotechnological route to the biosynthesis of value-added organofluorines. The fluorinase is kno...
The potential of bacteriophages to control Xanthomonas campestris pv. campestris at different stages of disease development [0.03%]
不同发病阶段丁香假单胞菌致病细菌控制中噬菌体的应用潜能研究
Dominique Holtappels,Kiandro J Fortuna,Lauren Moons et al.
Dominique Holtappels et al.
Xanthomonas campestris pv. campestris (Xcc) is a vascular pathogen that invades the xylem of Brassica crops. Current chemical and antibiotics-based control measures for this bacterium are unsustainable and inefficient. After establishing a ...
Development of a FungalBraid Penicillium expansum-based expression system for the production of antifungal proteins in fungal biofactories [0.03%]
基于青霉属醭曲霉的抗真菌蛋白质发酵工厂生产体系的研发
Mónica Gandía,Elena Moreno-Giménez,Moisés Giner-Llorca et al.
Mónica Gandía et al.
Fungal antifungal proteins (AFPs) have attracted attention as novel biofungicides. Their exploitation requires safe and cost-effective producing biofactories. Previously, Penicillium chrysogenum and Penicillium digitatum produced recombinan...
Enzyme-constrained models predict the dynamics of Saccharomyces cerevisiae growth in continuous, batch and fed-batch bioreactors [0.03%]
基于酶约束模型预测酵母在连续发酵、分批补料和分批发酵中的生长动力学
Sara Moreno-Paz,Joep Schmitz,Vitor A P Martins Dos Santos et al.
Sara Moreno-Paz et al.
Genome-scale, constraint-based models (GEM) and their derivatives are commonly used to model and gain insights into microbial metabolism. Often, however, their accuracy and predictive power are limited and enable only approximate designs. T...