The fluorescent protein iLOV as a reporter for screening of high-yield production of antimicrobial peptides in Pichia pastoris [0.03%]
作为筛选抗菌肽高效分泌生产工程菌株报告基因的荧光蛋白iLOV
Annemette Kjeldsen,Jack E Kay,Scott Baxter et al.
Annemette Kjeldsen et al.
The methylotrophic yeast Pichia pastoris is commonly used for the production of recombinant proteins at scale. The identification of an optimally overexpressing strain following transformation can be time and reagent consuming. Fluorescent ...
Juan L Ramos,Ben Pakuts,Patricia Godoy et al.
Juan L Ramos et al.
Much of the energy being used to power our lives comes from fossil fuels such as coal, natural gas and petroleum. These energy sources are non-renewable, are being exhausted and also pollute the air, water and soil with toxic chemicals. The...
Diverse responses of pqqC- and phoD-harbouring bacterial communities to variation in soil properties of Moso bamboo forests [0.03%]
土壤性质变化对毛竹林栖含pqqC和phoD基因细菌群落的影响不同
Wenhui Shi,Yijing Xing,Ying Zhu et al.
Wenhui Shi et al.
Phosphate-mobilizing bacteria (PMB) play a critical role in the regulation of phosphorus availability in the soil. The microbial genes pqqC and phoD encode pyrroloquinoline quinone synthase and bacterial alkaline phosphatase, respectively, ...
Enhancing the capability of Klebsiella pneumoniae to produce 1, 3-propanediol by overexpression and regulation through CRISPR-dCas9 [0.03%]
通过CRISPR-dCas9过表达和调控增强肺炎克雷伯氏菌生成1,3-丙二醇的能力
Xin Wang,Lin Zhang,Shaoxiong Liang et al.
Xin Wang et al.
Klebsiella pneumoniae is a common strain of bacterial fermentation to produce 1, 3-propanediol (1, 3-PDO). In general, the production of 1, 3-PDO by wild-type K. pneumoniae is relatively low. Therefore, a new gene manipulation of K. pneumon...
Pyocyanin biosynthesis protects Pseudomonas aeruginosa from nonthermal plasma inactivation [0.03%]
毗蓝蛋白生物合成保护铜绿假单胞菌免受非热等离子体灭活的影响
Huyue Zhou,Yi Yang,Weilong Shang et al.
Huyue Zhou et al.
Pseudomonas aeruginosa is an important opportunistic human pathogen, which raises a worldwide concern for its increasing resistance. Nonthermal plasma, which is also called cold atmospheric plasma (CAP), is an alternative therapeutic approa...
Vaccine with bacterium-like particles displaying HIV-1 gp120 trimer elicits specific mucosal responses and neutralizing antibodies in rhesus macaques [0.03%]
一种类似细菌的粒子型艾滋病疫苗诱发了恒河猴产生特异性的粘膜免疫反应和中和抗体
Huaiyu Wang,Pingchao Li,Mo Zhang et al.
Huaiyu Wang et al.
Preclinical studies have shown that the induction of secretory IgA (sIgA) in mucosa and neutralizing antibodies (NAbs) in sera is essential for designing vaccines that can effectively block the transmission of HIV-1. We previously showed th...
Microbial recycling of plastics: An annotated selection of World Wide Web sites relevant to the topics in microbial biotechnology: An annotated selection of World Wide Web sites relevant to the topics in microbial biotechnology [0.03%]
塑料的微生物降解与有关微生物技术主题的世界万维网网站：有关微生物技术主题的世界万维网网站的注释式选择
Lawrence P Wackett
Lawrence P Wackett
High antimicrobial activity of lactoferricin-expressing Bacillus subtilis strains [0.03%]
表达乳铁蛋白肽的枯草芽孢杆菌菌株具有高抗菌活性
Bing-Chang Lee,Jui-Che Tsai,Chun-Wei Hung et al.
Bing-Chang Lee et al.
The lactoferricin expressed in Bacillus subtilis is relatively low in yield, making it hard to apply in industrial settings. We constructed a six tandem repeat of lactoferricin cDNA driven by promoter PtrnQ. After transformation, two transf...
Detection and classification of SARS-CoV-2 using high-resolution melting analysis [0.03%]
基于高分辨率熔解分析的新型冠状病毒(SARS-CoV-2)检测与分型研究
Liying Sun,Leshan Xiu,Chi Zhang et al.
Liying Sun et al.
Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has recently posed a significant threat to global public health. The objective of this study was to develop and evaluate a...
Extracellular vesicle formation in Lactococcus lactis is stimulated by prophage-encoded holin-lysin system [0.03%]
乳链球菌中的胞外囊泡形成由前噬菌体编码的holin-溶菌酶系统刺激产生
Yue Liu,Marcel H Tempelaars,Sjef Boeren et al.
Yue Liu et al.
Gram-positive bacterial extracellular membrane vesicles (EVs) have been drawing more attention in recent years. However, mechanistic insights are still lacking on how EVs are released through the cell walls in Gram-positive bacteria. In thi...