Mining versus in vitro evolution for the selection of novel microbial insecticidal proteins [0.03%]
筛选新型微生物杀虫蛋白的挖掘与体外分子进化比较研究
Alejandra Bravo,Mario Soberón
Alejandra Bravo
An annotated selection of World Wide Web sites relevant to the topics in microbial biotechnology [0.03%]
与微生物技术主题相关的网络资源注释选编
Lawrence P Wackett
Lawrence P Wackett
Polyhydroxyalkanoate production from animal by-products: Development of a pneumatic feeding system for solid fat/protein-emulsions [0.03%]
动物副产品聚羟基脂肪酸酯的生产：固体脂肪/蛋白乳化物气动供料系统的开发
Björn Gutschmann,Thomas H Högl,Boyang Huang et al.
Björn Gutschmann et al.
Fat-containing animal by-product streams are locally available in large quantities. Depending on their quality, they can be inexpensive substrates for biotechnological processes. To accelerate industrial polyhydroxyalkanoate (PHA) bioplasti...
Current progress on engineering microbial strains and consortia for production of cellulosic butanol through consolidated bioprocessing [0.03%]
整合生物处理法制备纤维素丁醇的工程菌株及联合体的研究进展
Angela Re,Roberto Mazzoli
Angela Re
In the last decades, fermentative production of n-butanol has regained substantial interest mainly owing to its use as drop-in-fuel. The use of lignocellulose as an alternative to traditional acetone-butanol-ethanol fermentation feedstocks ...
Multiplex gene precise editing and large DNA fragment deletion by the CRISPR-Cas9-TRAMA system in edible mushroom Cordyceps militaris [0.03%]
CRISPR-Cas9-TRAMA系统在可食用虫草菌 Cordyceps militaris 中进行多重基因精准编辑和大片段DNA片段敲除
Bai-Xiong Chen,Ling-Na Xue,Tao Wei et al.
Bai-Xiong Chen et al.
The medicinal mushroom Cordyceps militaris contains abundant valuable bioactive ingredients that have attracted a great deal of attention in the pharmaceutical and cosmetic industries. However, the development of this valuable mushroom face...
Multi-level metabolic engineering of Escherichia coli for high-titer biosynthesis of 2'-fucosyllactose and 3-fucosyllactose [0.03%]
用于高滴度合成2'-岩藻糖基乳糖和3-岩藻糖基乳糖的多级代谢工程大肠杆菌
Mengli Li,Chenchen Li,Yejiao Luo et al.
Mengli Li et al.
Fucosyllactoses (FL), including 2'-fucosyllactose (2'-FL) and 3-fucosyllactose (3-FL), have garnered considerable interest for their value in newborn formula and pharmaceuticals. In this study, an engineered Escherichia coli was developed f...
The protein scaffold calibrates metal specificity and activation in MerR sensors [0.03%]
蛋白质支架校准MerR传感器中的金属特异性和活化性
Julián I Mendoza,Julián Lescano,Fernando C Soncini et al.
Julián I Mendoza et al.
MerR metalloregulators are the central components of many biosensor platforms designed to report metal contamination. However, most MerR proteins are non-specific. This makes it difficult to apply these biosensors in the analysis of real en...
The influence of H2 partial pressure on biogenic palladium nanoparticle production assessed by single-cell ICP-mass spectrometry [0.03%]
单细胞ICP质谱法评估H2分压对生物产钯纳米颗粒的影响
Cindy Ka Y Law,Eduardo Bolea-Fernandez,Tong Liu et al.
Cindy Ka Y Law et al.
The production of biogenic palladium nanoparticles (bio-Pd NPs) is widely studied due to their high catalytic activity, which depends on the size of nanoparticles (NPs). Smaller NPs (here defined as
Salinomycin biosynthesis reversely regulates the β-oxidation pathway in Streptomyces albus by carrying a 3-hydroxyacyl-CoA dehydrogenase gene in its biosynthetic gene cluster [0.03%]
盐霉素生物合成通过携带β氧化途径中的3-羟基酰基辅酶A脱氢酶基因逆向调节芽孢杆菌的β氧化路径
Jiaxiu Wei,Binbin Chen,Jianxin Dong et al.
Jiaxiu Wei et al.
Streptomyces is well known for synthesis of many biologically active secondary metabolites, such as polyketides and non-ribosomal peptides. Understanding the coupling mechanisms of primary and secondary metabolism can help develop strategie...
Vector redesign and in-droplet cell-growth improves enrichment and recovery in live Escherichia coli [0.03%]
向活体大肠杆菌中引入载体重构及胞内生长以改进富集和回收工作
Bernard D G Eenink,Tomasz S Kaminski,Erich Bornberg-Bauer et al.
Bernard D G Eenink et al.
Directed evolution (DE) is a widely used method for improving the function of biomolecules via multiple rounds of mutation and selection. Microfluidic droplets have emerged as an important means to screen the large libraries needed for DE, ...