Genome-Scale Engineering Importing Property of Escherichia coli for Improving Production of 3-Hydroxypropionic Acid [0.03%]
基于全局代谢网络优化的大肠杆菌异源生产3-羟基丙酸代谢工程研究
Seungjin Kim,Junyeong Sung,Ga Yeon Lim et al.
Seungjin Kim et al.
Microbial biochemical production can suffer reduced growth and productivity from intracellular product accumulation, which can be mitigated by minimizing product import. Limited understanding of import-related genes, especially for non-nati...
Identification and Characterisation of the Gene Cluster Governing Biosynthesis of the Anti-Mycobacterial Antibiotic Acidomycin [0.03%]
酸小杆菌素生物合成基因簇的鉴定与表征
Anna Vignolle,Martin Zehl,Jaime Felipe Guerrero Garzón et al.
Anna Vignolle et al.
Acidomycin is an anti-mycobacterial antibiotic with a unique mode of action, targeting the biotin biosynthesis pathway. Despite being highly active against mycobacteria in vitro, its development as an anti-tubercular agent has been hindered...
Construction and Biological Characterization of Papaya Leaf Curl Guangdong Virus Infectious cDNA Clone [0.03%]
番木瓜叶卷曲广东病毒感染性cDNA克隆的构建及生物学性质研究
Sheng Xu,Gaoli Wu,Zhaoyao Yu et al.
Sheng Xu et al.
Papaya leaf curl Guangdong virus (PaLCuGDV), a member of the genus Begomovirus, poses a significant threat to passion fruit (Passiflora edulis) cultivation. A recent field survey in the Rongjiang area of Guizhou Province revealed a high nat...
A Bait-and-Switch Strategy Links Phenotypes to Genes Coding for Polymer-Degrading Enzymes in Intact Microbiomes [0.03%]
一种易鱼诱骗策略将表型链接到完整微生物组中编码聚合物降解酶的基因
Colleen E Yancey,Kyle D Brumfield,Jackson A Buss et al.
Colleen E Yancey et al.
Natural microbial communities, with their vast diversity and complexity, are among the richest sources of untapped novel enzymes. Identifying novel enzymes can be challenging because microbiomes often lack clear, measurable phenotypes, unli...
From Energy Deprivation Stress to Catalytic Adaptation of Reductive Dehalogenase TmrA for 1,1-DCA Dechlorination: An Experimental and in Silico Study [0.03%]
从能量剥夺胁迫到脱卤酶TmrA催化适应于1,1-DCE脱氯：实验与基于计算化学的理论研究
Yi Ren,Evan Landers,Matthew Lee et al.
Yi Ren et al.
Organohalide-respiring bacteria capable of metabolizing multiple organohalogens represent valuable tools for bioremediation and offer intriguing evolutionary potential for adaptation to non-native substrates. In the present study, a chlorof...
Transglycosylation Capabilities of Wild-Type α-l-Fucosidase iso1 from Paenibacillus thiaminolyticus and Its Engineered Mutants: Preparation of Fucosylated Oligosaccharides [0.03%]
来自Paenibacillus thiaminolyticus的野生型α-L-岩藻糖苷酶iso1及其工程突变体的转糖基能力：制备岩藻糖化寡糖
Patricie Vodičková,Lucie Klimešová,Pavlína Nekvasilová et al.
Patricie Vodičková et al.
To enhance the yield of l-fucosylated molecules synthesized via transfucosylation, we employed α-l-transfucosidases, enzymes engineered from α-l-fucosidases to favour transglycosylation over hydrolysis. This study investigated the transgl...
Genetically Boosting Electron Transfer in Electroactive Biofilms for Improved Sensitivity of Microbial Fuel Cell-Based Biosensing [0.03%]
用于微生物燃料电池生物传感的导电菌落的遗传优化以提高电子转移效率和传感器灵敏度
Yutong Zhang,Xi Han,Yongguang Jiang et al.
Yutong Zhang et al.
Electroactive biofilms (EABs) hold significant potential for applications in bioenergy, biosensing and wastewater treatment; however, their performance is frequently hindered by inefficient extracellular electron transfer (EET). A notable e...
A Manifesto for Universal Healthcare: Reconstituting Primary Care Through Digital Innovation, Microbial Technologies and Empowered Citizenship [0.03%]
全民医疗宣言——通过数字化创新、微生物技术及公民赋权重塑初级保健体系
Kenneth Timmis,Gerard Clarke,María Francisca Colom et al.
Kenneth Timmis et al.
Despite unprecedented medical advances, global healthcare systems are failing to deliver universal, equitable and quality care. Many systems also have low resilience to surges in demand, are highly fragmented, or suffer from unsustainable f...
Recovery and Survival of Prototheca Microalgae During Wastewater Treatment Process [0.03%]
prototheca 微藻在污水处理过程中的复苏和存活研究
Mateusz Iskra,Marta Romaszuk,Mariusz Dyląg et al.
Mateusz Iskra et al.
Prototheca are unicellular, non-pigmented, yeast-like microalgae widely distributed in natural and anthropogenic environments. While typically saprophytic, these organisms are the only plant species known to have emerged as opportunistic pa...
Cryo-EM Structures of Alcohol Oxidase Isozymes Reveal Structural Determinants of Cofactor Variation and Enzymatic Activity in Ogataea methanolica [0.03%]
低温电子显微镜下的乙醇脱氢酶同工酶结构揭示了Ogataea methanolica中辅因子差异和酶活性的结构决定因素
Hao-Liang Cai,Atsuhiro Shimada,Tasuku Hamaguchi et al.
Hao-Liang Cai et al.
Ogataea methanolica is a methylotrophic yeast that can produce diverse recombinant proteins using methanol as the sole carbon and energy source. Unlike most yeast species, which possess a single alcohol oxidase, O. methanolica encodes two i...