Engineering Chondroitinase-Free Baculovirus-Insect Cell Expression System for Efficient Synthesis of Chondroitin Sulphates [0.03%]
用于高效合成硫酸软骨素的无软骨素酶的杆状病毒-昆虫细胞表达系统工程化
Junyue Li,Yuqing Tian,Lihe She et al.
Junyue Li et al.
Owing to the high expression levels, proper protein folding and post-translational modifications, baculovirus-insect cell expression system is an ideal platform for producing active chondroitin sulfotransferases, which specifically catalyse...
Lineage-Specific Adaptive Strategies Shape the Response of de Novo Lager Yeast Hybrids to High-Gravity Fermentation [0.03%]
种属特异性适应策略塑造了新生的拉格酵母杂交菌对高 gravity 发酵的响应
Sonia Albillos-Arenal,Vasni Zavaleta,Fernanda Jeria et al.
Sonia Albillos-Arenal et al.
Lager beer is the most widely consumed fermented beverage worldwide and is produced by the yeast Saccharomyces pastorianus, which originated from hybridization between S. cerevisiae (Sc) and S. eubayanus (Se). Although the genetic factors u...
Cutibacterium acnes-Derived Extracellular Vesicles Promote Epithelial Ovarian Cancer Progression by Activating the KEAP1-NRF2 Antioxidant Pathway to Suppress Ferroptosis [0.03%]
微菌簇痤疮胞外囊泡通过激活KEAP1-NRF2抗氧化通路抑制铁死亡从而促进卵巢癌进展
Qifa Huang,Qi Chen,Wenjie Xiong et al.
Qifa Huang et al.
Bacterial extracellular vesicles are increasingly recognized as important mediators of microbe-host communication, yet their functional roles within tumour-associated microbiota remain poorly understood. Here, we investigated whether extrac...
The Arsenal of Aromatic Degrading Bacteria: How They Sense, Chase, Adapt and Destroy Environmental Pollutants [0.03%]
芳香降解菌的武器库：它们如何感知、追踪、适应和破坏环境污染物
Prashant S Phale,Tushar Dhamale,Satyajit Subhash
Prashant S Phale
Aromatic compounds are ubiquitous arising from natural sources as well as anthropogenic activities posing significant ecological and health risks due to their persistence and toxicity in nature. While bacterial biodegradation of these compo...
Machine Learning Reveals Quantitative Amino Acid Preferences in Bifidobacterium longum Growth [0.03%]
机器学习揭示长双歧杆菌生长中的氨基酸定量偏好
Hiroki Kaneko,Kana Kadowaki,Shin Yoshimoto et al.
Hiroki Kaneko et al.
Bifidobacterium longum is a prevalent human gut symbiont whose carbohydrate metabolism is well characterized, whereas the quantitative contribution of amino acids to growth remains unclear. Here, we combined genome-based pathway analysis, g...
Followers' Choice: The Trends Transforming Precision Medicine, Synthetic Biology, and Sustainable Microbiology [0.03%]
精准医学、合成生物学和可持续微生物学的发展趋势
Patricia Bernal,Rocío Palacios-Ferrer,Juan L Ramos
Patricia Bernal
Characterization of the Orphan Cytochrome P450 CYP107J1 From Bacillus subtilis Through Peroxygenase Activity Engineering [0.03%]
通过过氧化酶活性工程表征孤儿细胞色素P450 CYP107J1（来源于枯草芽孢杆菌）
Hideki Kato,Takafumi Hashimoto,Tuhin Das et al.
Hideki Kato et al.
Cytochrome P450 monooxygenases (P450s) not only play many physiological roles in oxidative metabolism but are also promising biocatalysts for the synthesis of organic molecules. Bacillus subtilis strain 168, which possesses eight P450 genes...
A Novel Small Molecule Accelerates Early Persister Regrowth and Potentiates Antibiotic Killing via MdtL-DcrB [0.03%]
一种新型小分子通过MdtL-DcrB促进早期休眠菌的复苏并增强抗生素杀菌能力
Garin Park,Hyein Kim,Sooyeon Song
Garin Park
Persister cells survive antibiotic exposure through transient tolerance, often leading to infection relapse. Because antibiotic susceptibility is restored when persisters resume growth, we sought to identify a chemical modulator that advanc...
Tenebrio molitor-Derived Enzyme Systems Enable Solvent-Reduced Recovery of Intracellular Polyhydroxyalkanoates [0.03%]
苔虫酶系统实现低溶剂含量条件下内源PHA的高效提取及应用研究
Sanja Skaro Bogojevic,Brana Pantelic,Marijana Ponjavic et al.
Sanja Skaro Bogojevic et al.
Polyhydroxyalkanoates (PHAs) are intracellular microbial polyesters whose commercial deployment is strongly influenced by downstream processing costs and environmental burden. Here, we demonstrate a nearly solvent-free, mild aqueous strateg...
Christoph Gunkel,Bastian Blombach
Christoph Gunkel
Succinic acid has been considered an important molecule in the transition of chemical manufacturing from fossil-based to sustainable and future-proof processes. While there has been extensive research on biotechnological succinic acid produ...