COVID-19: emergence and mutational diversification of SARS-CoV-2 [0.03%]
COVID-19：SARS-CoV-2的出现及其突变多样性
Harald Brüssow
Harald Brüssow
The origin of the SARS-CoV-2 virus is not yet defined, but a viral zoonosis from bats - with or without an alternative animal as an intermediate host - is still the most likely hypothesis. The intensive virological and epidemiological resea...
Increased xerotolerance of Saccharomyces cerevisiae during an osmotic pressure ramp over several generations [0.03%]
在多次世代中，渗透压逐渐升高的过程中Saccharomyces cerevisiae的耐干性增加
Stéphane Guyot,Laurence Pottier,Lucie Bertheau et al.
Stéphane Guyot et al.
Although mechanisms involved in response of Saccharomyces cerevisiae to osmotic challenge are well described for low and sudden stresses, little is known about how cells respond to a gradual increase of the osmotic pressure (reduced water a...
Bacterial fermentation and isotope labelling optimized for amyloidogenic proteins [0.03%]
优化的细菌发酵和同位素标记方法及其在淀粉样蛋白研究中的应用
István Vida,Zsolt Fazekas,Gergő Gyulai et al.
István Vida et al.
We developed a cost sensitive isotope labelling procedure using a fed-batch fermentation method and tested its efficiency producing the 15 N-, 13 C- and 15 N/13 C-labelled variants of an amyloidogenic miniprotein (E5: EEEAVRLYIQWLKEGGPSSGRP...
Unravelling the thioesterases responsible for propionate formation in engineered Pseudomonas putida KT2440 [0.03%]
解码工程假单胞菌KT2440中丙酸形成的硫酯酶基因簇
Chao Ma,Ya&#x;nan Shi,Qingxuan Mu et al.
Chao Ma et al.
Pseudomonas putida KT2440 is becoming a new robust metabolic chassis for biotechnological applications, due to its metabolic versatility, low nutritional requirements and biosafety status. We have previously engineered P. putida KT2440 to b...
Young-Tae Park,Taejung Kim,Jungyeob Ham et al.
Young-Tae Park et al.
Faecalibacterium prausnitzii (F. prausnitzii) is one of the most abundant bacteria in the human intestine, with its anti-inflammatory effects establishing it as a major effector in human intestinal health. However, its extreme sensitivity t...
Cecal microbial transplantation attenuates hyperthyroid-induced thermogenesis in Mongolian gerbils [0.03%]
盲肠微生物移植可减轻蒙古沙鼠甲亢性产热增强现象
Saeid Khakisahneh,Xue-Ying Zhang,Zahra Nouri et al.
Saeid Khakisahneh et al.
Endothermic mammals have a high energy cost to maintain a stable and high body temperature (Tb , around 37°C). Thyroid hormones are a major regulator for energy metabolism and Tb . The gut microbiota is involved in modulating host energy m...
Biotechnological synthesis of Pd/Ag and Pd/Au nanoparticles for enhanced Suzuki-Miyaura cross-coupling activity [0.03%]
用于增强Suzuki-Miyaura交叉偶联活性的Pd/Ag和Pd/Au纳米颗粒的生物合成
Richard L Kimber,Fabio Parmeggiani,Thomas S Neill et al.
Richard L Kimber et al.
Bimetallic nanoparticle catalysts have attracted considerable attention due to their unique chemical and physical properties. The ability of metal-reducing bacteria to produce highly catalytically active monometallic nanoparticles is well k...
Attenuator LRR - a regulatory tool for modulating gene expression in Gram-positive bacteria [0.03%]
衰减器LRR——调控革兰氏阳性菌基因表达的工具
Xia Cai,Qian Wang,Yu Fang et al.
Xia Cai et al.
With the rapid development of synthetic biology in recent years, particular attention has been paid to RNA devices, especially riboswitches, because of their significant and diverse regulatory roles in prokaryotic and eukaryotic cells. Due ...
Rock phosphate solubilization by abiotic and fungal-produced oxalic acid: reaction parameters and bioleaching potential [0.03%]
化学和真菌源草酸溶解磷矿的作用参数及生物浸出潜力
Gilberto de Oliveira Mendes,Thomas Dyer,Laszlo Csetenyi et al.
Gilberto de Oliveira Mendes et al.
Oxalic acid-producing fungi play an important role in biogeochemical transformations of rocks and minerals and possess biotechnological potential for extraction of valuable elements from primary or waste ores and other solid matrices. This ...
A plasmid toolset for CRISPR-mediated genome editing and CRISPRi gene regulation in Escherichia coli [0.03%]
用于大肠杆菌基因组编辑和CRISPR干扰的质粒工具集
Adrian J Jervis,Erik K R Hanko,Mark S Dunstan et al.
Adrian J Jervis et al.
CRISPR technologies have become standard laboratory tools for genetic manipulations across all kingdoms of life. Despite their origins in bacteria, the development of CRISPR tools for engineering bacteria has been slower than for eukaryotes...