Reduced Genetic Heterogeneity for Stable Bioproduction by Harnessing the Bias and Mechanism of Mutation [0.03%]
利用突变的偏向性和机制降低遗传异质性以实现稳定的生物生产
Yanting Cao,Yaokang Wu,Xueqin Lv et al.
Yanting Cao et al.
Microbial bioproduction is an important approach to realising green biomanufacturing. However, poor bioproduction stability caused by genetic heterogeneity is one of the important factors limiting its industrial-scale applications. Here, tw...
Lawrence P Wackett
Lawrence P Wackett
Microbial metabolism of PFAS such as perfluorooctanoic acid (PFOA) or perfluorooctanesulfonic acid (PFOS) would yield 15 toxic fluoride anions and metabolites with relatively low growth yield coefficients. ...
Indole Derivatives Biosynthesis in Bifidobacterium longum subsp. infantis and the Tryptophan Substrate Availability [0.03%]
婴幼儿双歧杆菌吲哚衍生物的生物合成及其色氨酸底物的可用性
Shi-Min Zhang,Hui-Chu Wu,Jia-He Hung et al.
Shi-Min Zhang et al.
The metabolic processes of Bifidobacterium longum subsp. infantis, an early coloniser of the human gut, are essential for gut health, mainly due to the production of indole derivatives from tryptophan. This study investigates the capacity o...
Rapid Identification and Typing of Carbapenem-Resistant Klebsiella pneumoniae Using MALDI-TOF MS and Machine Learning [0.03%]
使用基质辅助激光解析电离飞行时间质谱和机器学习快速识别和分型碳青霉烯耐药肺炎克雷伯菌
Zhiyi Ye,Jin Zhu,Yang Liu et al.
Zhiyi Ye et al.
Use matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) to screen the specific mass peaks of carbapenem-resistant Klebsiella pneumoniae (CRKP), compare the differences in spectrum peaks between intest...
Public Health Microbiome Curriculum: Looking Below the Tip of the Iceberg for Approaches to Population Health [0.03%]
公共卫生微生物组课程：探究群体健康的方法冰山一角下的世界
Melissa K Melby,Swapna Mylabathula,Meghan B Azad et al.
Melissa K Melby et al.
We discuss the opportunity for public health microbiome curricula to bridge the gaps in knowledge that exist between microbiome researchers and the lay public. We propose equipping public health professionals, important facilitators of publ...
Probiotic-Based Approaches for Sustainable Control of Infectious Risk in Mass Transport: Current Data and Future Perspectives [0.03%]
基于益生菌的可持续控制大规模交通运输感染风险的方法：现有数据及未来前景
Irene Soffritti,Maria D&#x;Accolti,Francesca Bini et al.
Irene Soffritti et al.
The built environments of high-traffic areas can play a significant role in the transmission of microorganisms and associated infections, sometimes favouring the selection of multidrug-resistant (MDR) organisms due to the excessive use of c...
Methanol-Free Protein Expression in Komagataella phaffii With Magnetic or Non-Magnetic Heating [0.03%]
无甲醇蛋白表达：以磁或非磁加热的毕赤酵母Komagataella phaffii
Ibrahim Dagci,Seyda Yildiz Arslan,Kubra Solak et al.
Ibrahim Dagci et al.
Komagataella phaffii is among the most widely used expression systems, with methanol-inducible promoters being preferred for protein expression due to their stringent regulation and exceptional strength. However, the applicability of this s...
Revitalising Brewers' Spent Grains and Enriching With Biogenic Compounds Through the Fermentation of Fructophilic Lactic Acid Bacteria and Yeasts [0.03%]
通过果糖乳酸菌和酵母的发酵活化啤酒糟并富集生物合成化合物
Alessandro Stringari,Ali Zein Alabiden Tlais,Stefano Tonini et al.
Alessandro Stringari et al.
The large output of spent grains from the brewing industry presents environmental concerns but also offers promising nutritional and functional potential for valorization by researchers and industrial stakeholders. In this perspective, we i...
Sergio Martínez-Rodríguez,Jose Antonio Gavira
Sergio Martínez-Rodríguez
N-Acyl-D-amino acid deacylases (EC 3.5.1.81, also known as D-acylases) have been studied for decades for their utility in the kinetic resolution of N-acetyl-D,L-amino acids (NAAs) due to a marked stereospecificity. In conjunction with an N-...
Toehold Switch-Based Approach for Engineering Acid-Tolerance Modules to Enhance Production Robustness of Industrial E. coli Strains at Low pH [0.03%]
基于脚手架开关的工程酸耐受模块以增强工业大肠杆菌菌株在低pH值下的生产鲁棒性方法
Xin Zhang,Xiaofang Yan,Peng Liu et al.
Xin Zhang et al.
Enhancing acid tolerance of industrial microorganisms is critical for improving fermentation efficiency and sustainability. This study presents a synthetic biology approach that employs toehold switch-based acid-tolerance modules to enginee...