Oleksandr Berezin,Alberto Piovesan,Roman Graf et al.
Oleksandr Berezin et al.
This protocol provides comprehensive guidance for researchers, including those with limited electron microscopy (EM) experience, on the effective use of genetically encoded, multichannel EMcapsulin reporters in both fluorescence microscopy ...
Directional conjugation of monoclonal antibodies to nanoparticles using metal-free click chemistry [0.03%]
一种基于无金属点击化学的单克隆抗体定向结合纳米粒子的方法
Ananthakrishnan S Jeevarathinam,Mohammed R Kawelah,Sangheon Han et al.
Ananthakrishnan S Jeevarathinam et al.
Here, we describe a practical step-by-step protocol for directional conjugation of monoclonal antibodies to polymersome nanoparticles via the Fc antibody moiety using metal-free click chemistry. This Protocol Extension details procedures fo...
Multilevel characterization of genome editor nuclease activity with BreakTag [0.03%]
利用BreakTag对基因组编辑核酸酶活性进行多层次表征
Gabriel M C Longo,Sergi Sayols,Vassilis Roukos
Gabriel M C Longo
BreakTag is a scalable next-generation sequencing-based method for the unbiased characterization of programmable nucleases and guide RNAs at multiple levels. BreakTag allows off-target nomination, nuclease activity assessment and the charac...
Proteome-wide profiling of S-nitrosylated proteins using the SNOTRAP probe and mass spectrometry-based detection [0.03%]
基于质谱检测的SNOTRAP探针诱导的蛋白质硫氮杂环乙烷化修饰整体蛋白质组学分析方法研究
Hongmei Yang,Haitham Amal,Steven R Tannenbaum et al.
Hongmei Yang et al.
Protein S-nitrosylation (SNO) is a ubiquitous post-translational modification, which regulates a broad range of functional parameters, including protein stability; enzymatic, transcriptional and ion channel activity; and cellular signal tra...
Zhuning Wang,Sijie Pian,Yaoguang Ma
Zhuning Wang
Radiative cooling is a renewable cooling mechanism with diverse applications. Many radiative cooling materials have been developed (e.g., fabrics, paints and building materials). It is, however, difficult to compare their properties because...
Extracting and characterizing microplastics and nanoplastics from environmental samples [0.03%]
从环境样品中提取和表征微塑料和纳米塑料
Nana Liu,Zifu Li,Shikun Cheng et al.
Nana Liu et al.
There is increasing interest in measuring the effect of microplastics and nanoplastics (MNPs) in the environment, but it is difficult to compare the results obtained in these studies due to variations in the extraction and characterization ...
Branching beyond bifunctional linkers: synthesis of macrocyclic and trivalent PROTACs [0.03%]
超越双官能连接臂的分支：大环和三价PROTACs的合成
Yuting Cao,Alexandra L Harris,Alessio Ciulli
Yuting Cao
Proteolysis-targeting chimeras (PROTACs) are traditionally conceptualized and synthesized by connecting two ligands, one for a target protein and one for an E3 ligase, via a bifunctional linker. Chemical creativity has recently explored the...
Production of human blood-generating heart-forming organoids and sample preparation for advanced imaging [0.03%]
人血液生成心脏形成器官体的制备及用于高级成像的样本准备
Miriana Dardano,Liam Wilson,Robert Zweigerdt et al.
Miriana Dardano et al.
Human pluripotent stem (hPS) cell-derived blood-generating heart-forming organoids (BG-HFOs) represent a highly structured in vitro model that recapitulates aspects of human cardiac, hematoendothelial and multiwave hematopoietic co-developm...
Comparing methods for preparing slippery liquid-like polydimethylsiloxane coatings [0.03%]
比较制备滑爽的聚二甲基硅氧烷涂层的方法
Isaac J Gresham,Hernán Barrio-Zhang,Jae Hyung Cho et al.
Isaac J Gresham et al.
Slippery covalently attached liquid surfaces (SCALS, also called quasi-liquid surfaces, liquid-like surfaces and slippery omniphobic covalently attached surfaces) have recently emerged as a new family of materials with many useful propertie...
Publisher Correction: NanoVar: a comprehensive workflow for structural variant detection to uncover the genome's hidden patterns [0.03%]
出版更正：NanoVar：一个全面的工作流程，用于检测结构变异以揭示基因组的隐藏模式
Asmaa Samy,Cheng Yong Tham,Matthew Dyer et al.
Asmaa Samy et al.
Published Erratum
Nature protocols. 2025 Oct 16. DOI:10.1038/s41596-025-01297-8 2025