Quantifying the severity of Marssonina blotch on apple leaves: development and validation of a novel spectral index [0.03%]
马拉松斑病对苹果叶片严重程度的量化：新型光谱指数的发展与验证
Wenjie Zhang,Chengjian Zhang,Riqiang Chen et al.
Wenjie Zhang et al.
Apple Marssonina blotch (AMB) is a major disease causing pre-mature defoliation. The occurrence of AMB will lead to serious production decline and economic losses. The precise identification of AMB outbreaks and the measurement of its sever...
Establishment of a low-temperature immersion method for extracting high-activity and high-purity mitochondria from Syntrichia caninervis Mitt [0.03%]
建立从Syntrichia caninervis Mitt中提取高活性和高纯度线粒体的低温浸提法
Wenting Huo,Xiaohua Lin,Mengyu Gao et al.
Wenting Huo et al.
Background: Mitochondria are central to plant growth, development, and stress resilience. Despite their importance, mitochondrial research in desiccation-tolerant mosses remains underexplored. To unravel the stress resist...
A reproducible ddRAD-seq protocol reveals novel genomic association signatures for fruit-related traits in peach [0.03%]
一种可重复的ddRAD-seq协议揭示了桃果实相关性状的新基因组关联信号
Najla Ksouri,Gerardo Sánchez,Carolina Font I Forcada et al.
Najla Ksouri et al.
Improving peach cultivars with superior traits is a primary objective of breeding initiatives. In this study, we aimed to elucidate the genetic basis of key agronomic and fruit-related traits using a reproducible ddRAD-seq protocol applied ...
Widely-based full-genome analyses enable development of universal and strain-specific PCR toolkit for wheat dwarf virus detection, revealing new alternative hosts and challenging strain-host specificity [0.03%]
基于广泛的全基因组分析开发针对小麦矮缩病毒检测的通用型和专一性PCR工具包，并揭示了新的替代寄主及挑战毒株-寄主特异性关系
Botond Zsombor Pertics,Gergely Tholt,András Kis et al.
Botond Zsombor Pertics et al.
Background: Wheat dwarf virus (WDV) is a destructive cereal virus causing significant yield losses in wheat and barley. It is transmitted by the leafhopper Psammotettix alienus and can persist in wild grasses between grow...
Development of a multi-targeted real-time PCR assay for the detection of the grapevine pathogen Xylophilus ampelinus [0.03%]
葡萄vine病原物Xylophilus ampelinus多重实时PCR检测方法的建立
Aleksander Benčič,Alexandra Bogožalec Košir,Janja Matičič et al.
Aleksander Benčič et al.
Background: Xylophilus ampelinus is a plant pathogenic bacterium that causes bacterial blight in grapevines, which can lead to severe yield losses and economic damage. Owing to its fastidious growth on culture media, dete...
A simple and low-cost method for fluoride analysis of plant materials using alkali extraction and ion-selective electrode [0.03%]
一种利用碱提取和离子选择电极分析植物样品中氟含量的简便低成本方法
Chenyu Zhang,Mark G M Aarts,Antony van der Ent
Chenyu Zhang
Backgrounds: Existing methods for fluoride (F-) determination in plant material require expensive equipment and specialized reagents. This study aimed to develop a simple and cost-effective method for fluoride analysis in...
Ionic liquid-assisted seed genomic DNA extraction for advanced sequencing applications [0.03%]
用于高级测序应用的离子液体辅助种子基因组DNA提取
Shashini De Silva,Philip C Bentz,Cecilia Cagliero et al.
Shashini De Silva et al.
Background: Modern plant breeding strategies rely on the intensive use of advanced genomic tools to expedite the development of improved crop varieties. Genomic DNA extraction from crop seeds eliminates the need to grow p...
pSIG plasmids, MoClo-compatible vectors for efficient production of chimeric double-stranded RNAs in Escherichia coli HT115 (DE3) strain [0.03%]
pSIG载体，一种用于在大肠杆菌HT115（DE3）菌株中有效生产嵌合型双链RNA的MoClo兼容质粒
Ching-Feng Wu,Li-Pang Chang,Chan Lee et al.
Ching-Feng Wu et al.
Background: Spray-induced gene silencing (SIGS) is a promising strategy for controlling plant diseases caused by pests, fungi, and viruses. The method involves spraying on plant surfaces double-stranded RNAs (dsRNAs) that...
A new phenotyping method for root growth studies in compacted soil validated by GWAS in barley [0.03%]
一种在土壤压实条件下研究根系生长的新表型方法，并通过大麦的全基因组关联分析进行验证
Giorgia Carletti,Agostino Fricano,Elisabetta Mazzucotelli et al.
Giorgia Carletti et al.
Background: Soil compaction is defined as the reduction of air-filled pore space affecting soil density, water conductivity and nutrient availability. These conditions negatively influence root morphology, root developmen...
Stomata morphology measurement with interactive machine learning: accuracy, speed, and biological relevance? [0.03%]
交互式机器学习在气孔形态测量中的准确性、速度和生物学相关性？
Tomke S Wacker,Abraham G Smith,Signe M Jensen et al.
Tomke S Wacker et al.
Stomatal morphology plays a critical role in regulating plant gas exchange influencing water use efficiency and ecological adaptability. While traditional methods for analyzing stomatal traits rely on labor-intensive manual measurements, ma...