Predicting rice drought-responsive genes via distance-based prototypical graph neural network with path aggregation mechanism [0.03%]
Jing Liu,Hongyan Zhang,Song Wang et al.
Jing Liu et al.
Drought is a primary factor that adversely impacts rice yield and quality. Identifying drought-responsive genes is essential for developing drought-responsive cultivars. Recently, graph neural network methods based on embedding learning hav...
An efficient clear-native PAGE-based workflow for cryo-electron microscopy sample preparation of large protein complexes [0.03%]
一种高效的用于大型蛋白复合物冷冻电镜样品制备的clear-native page工艺流程
Zitong Yang,Shinsa Kameo,Soichiro Seki et al.
Zitong Yang et al.
Background: Cryo-electron microscopy (cryo-EM) has revolutionized protein research by enabling high-resolution structural analysis. However, preparing ultra-large protein complexes (e.g., > 700 kDa) for cryo-EM remains ch...
Non-destructive monitoring of root biomass in hydroponically grown leafy vegetables: comparison between machine learning-based RGB and hyperspectral imaging [0.03%]
基于机器学习的RGB和高光谱成像在叶菜水培生长中的非破坏性根系生物量监测比较研究
Ziyi Jin,Daisuke Yasutake,Tadashige Iwao et al.
Ziyi Jin et al.
Background: Root biomass serves as a critical indicator of plant eco-physiological status and crop productivity, yet its non-destructive monitoring remains challenging because of its underground location. The use of trans...
A high-throughput fluorescence-based microplate reader assay to quantify total flavonol levels in plant tissues [0.03%]
一种高通量荧光检测法测定植物组织中总黄酮醇含量
Hana Daryanavard,Joëlle K Muhlemann
Hana Daryanavard
Background: Flavonols are plant specialized metabolites that regulate plant growth, development, and stress responses. Due to their antioxidant activity, they also confer nutritional benefits to human health. Quantificati...
FE-SEM visualization of cortical microtubules in plant cells using freeze-fracture techniques [0.03%]
利用冷冻断裂技术在植物细胞中可视化皮层微管的场发射扫描电子显微镜观测
Tomohiro Hatano,Satoshi Nakaba,Hikaru Nagata et al.
Tomohiro Hatano et al.
Background: Cortical microtubules (CMTs), one of the components of cytoskeleton, control the orientation and localization of newly deposited cellulose microfibrils in cell walls, and thereby determine the shape, size, and...
Satinderpal Kaur,Devi Balakrishnan,Rupesh Kariyat
Satinderpal Kaur
Trichomes are hairlike protuberances in plants that deter herbivores using both physical and chemical means, with pre- and post-ingestive effects. In addition, trichomes also serve as a protective layer, reducing transpiration loss, thereby...
A simple and high-throughput single pollen genotyping pipeline to study meiosis in apple (Malus x domestica) [0.03%]
一种简单高效的单粒花粉基因分型方法以研究苹果减数分裂机制的研究
Han Palmers,Lucas Philips,Nico De Storme
Han Palmers
Background: Pollen are the primary carrier of male genetic information during sexual reproduction. In Angiosperms, pollen are produced in the anthers following the process of meiosis, during which ploidy halving and genet...
Real-time identification and quantification of apple scab on fruit in preharvest and postharvest conditions using YOLO11: a deep learning approach [0.03%]
基于YOLOv11的深度学习方法实现苹果采前采后果实病害黑星病的实时识别与分级研究
Fernanda Leiva,Séverine Gabioud Rebeaud,Danilo Christen
Fernanda Leiva
Background: Apple scab (AS), caused by the fungal pathogen Venturia inaequalis, is a major disease of apple that manifests as lesions on leaves and fruits. The disease compromises fruit quality and yield, leading to subst...
tRNA-based polycistronic CRISPR/Cas9 system boosts efficiency of multi-gene deletion in the moss Physcomitrella [0.03%]
基于tRNA的多顺反子CRISPR/Cas9系统提升苔类植物Physcomitrella多基因敲除效率
Elena Kozgunova
Elena Kozgunova
CRISPR/Cas9-based genome editing in the model bryophyte Physcomitrium patens (commonly known as Physcomitrella) is widely used for gene knockout via small insertions or deletions (indels). In this study, we developed an efficient dual-gRNA ...
SCFM-DETR: an enhanced transformer-based method for automated maize disease detection in field environments [0.03%]
一种基于变压器的改进方法，用于田间环境中自动检测玉米病害(SCFM-DETR)
Sasa Tian,Zhiqing Tao,Ke Li et al.
Sasa Tian et al.
Maize is susceptible to various diseases throughout its growth cycle, which can significantly reduce yields. The accurate identification of maize diseases with similar symptomatic manifestations is particularly challenging under field condi...