Magnetic nanoparticles-based digestion and enrichment methods in proteomics analysis [0.03%]
基于磁性纳米颗粒的蛋白质组学分析中的消化与富集方法
Mingxia Gao,Chunhui Deng,Xiangmin Zhang
Mingxia Gao
In proteome research, rapid and effective proteolysis and enrichment strategies are essential for successful protein identification. Functionalized magnetic microspheres of micro- and nano-meter size are gaining increasing attention due to ...
Jatin G Burniston,Eric P Hoffman
Jatin G Burniston
Regular exercise is effective in the prevention of chronic diseases and confers a lower risk of death in individuals displaying risk factors such as hypertension and dyslipidemia. Thus, knowledge of the molecular responses to exercise provi...
Human urine proteomics: building a list of human urine cancer biomarkers [0.03%]
人尿蛋白组学：构建人尿源癌症生物标志物列表
Juan Casado-Vela,Teresa Gómez del Pulgar,Arancha Cebrián et al.
Juan Casado-Vela et al.
In the last decade, several reports have focused on the identification and characterization of proteins present in urine. In an effort to build a list of proteins of interest as biomarkers, we reviewed the largest urine proteomes and built ...
Advantages of mRNA display selections over other selection techniques for investigation of protein-protein interactions [0.03%]
探究蛋白质间相互作用的mRNA展示筛选技术的优势其他筛选技术的优势
Hui Wang,Rihe Liu
Hui Wang
mRNA display is a genotype-phenotype conjugation method that allows for amplification-based, iterative rounds of in vitro selection to be applied to peptides and proteins. mRNA display can be used to display both long natural protein and sh...
Proteomics moves from expression to turnover: update and future perspective [0.03%]
蛋白质组学从表达到周转：更新与未来展望
Mary K Doherty,Phillip D Whitfield
Mary K Doherty
Proteomics is a rapidly developing discipline that seeks to understand the role of proteins in the wider biological context. In order to take a holistic view of a biological system, it is vital that we can elucidate the dynamics of the prot...
Capitalizing on the hydrophobic bias of electrospray ionization through chemical modification in mass spectrometry-based proteomics [0.03%]
利用质谱技术对蛋白质进行化学修饰以充分发挥电喷雾离子化中的疏水优势
Christopher M Shuford,David C Muddiman
Christopher M Shuford
Protein chemical derivatization has emerged as an invaluable bioanalytical approach in mass spectrometry-based proteomics with nearly unlimited potential. To date, derivatization strategies in proteomics have primarily focused on improving ...
Searching for biomarkers: humoral response profiling with luciferase immunoprecipitation systems [0.03%]
寻找生物标志物：含密度过滤单元的虫洞免疫沉淀系统进行体液反应检测
Peter D Burbelo,Kathryn H Ching,Kathleen E Bren et al.
Peter D Burbelo et al.
B-cell-mediated humoral responses are triggered in many human diseases, including autoimmune diseases, cancer, and neurologic and infectious diseases. However, the full exploitation of the information contained within a patient's antibody r...
Latex beads internalization and quantitative proteomics join forces to decipher the endosomal proteome [0.03%]
latex珠内吞作用与定量蛋白质组学联手解密胞内 vesicle 蛋白质组
Mariana Eça Guimarães de Araújo,Lukas Alfons Huber,Taras Stasyk
Mariana Eça Guimarães de Araújo
The proteome analysis of endocytic compartments has been constrained by the limited purity of the organelle fractions obtained by current biochemical methods. Duclos and coworkers have developed a novel method to isolate highly purified end...
Antonia Vlahou
Antonia Vlahou
Virginie Licker,Pierre R Burkhard
Virginie Licker