Novel activity assay for botulotoxin A1 detection using functionalized chips and matrix-assisted laser desorption/ionization mass spectrometry [0.03%]
利用功能化芯片和基质辅助激光解吸/电离质量谱检测肉毒杆菌毒素A1的新活性测定法
Zuzana Kalaninova,Josef Dvorak,Jiri Dresler et al.
Zuzana Kalaninova et al.
Background: Botulinum neurotoxins (BoNTs) are a group of neurotoxins produced by Clostridium bacteria. Their effect on neuro-muscular connections through cleaving proteins of the SNARE complex results in blocking acetylch...
Emerging protein sequencing technologies: proteomics without Mass spectrometry? [0.03%]
新兴的蛋白质测序技术：不需要质谱仪的蛋白质组学？
A S Deshpande,A Lin,I O&#x;Bryon et al.
A S Deshpande et al.
Introduction: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been a leading method for proteomics for thirty years. Advantages provided by LC-MS/MS are offset by significant disadvantages, including cost. R...
Elena Anaya-Cubero,Paz Cartas-Cejudo,Marina De Miguel et al.
Elena Anaya-Cubero et al.
Tobias Meisinger,Amelie Vogt,Robin Kretz et al.
Tobias Meisinger et al.
Introduction: Ligand binding assays combining immunoaffinity enrichment steps with mass spectrometry (MS) readout have gained attention as a highly specific and sensitive tool for protein quantification. These techniques ...
Omics and rare diseases: challenges, applications, and future perspectives [0.03%]
组学与罕见病：挑战、应用及未来展望
Daniela Braconi,Haidara Nadwa,Giulia Bernardini et al.
Daniela Braconi et al.
Introduction: Rare diseases (RDs) are a heterogeneous group of diseases recognized as a relevant global health priority but posing aspects of complexity such as: geographical scattering of affected individuals, improper/l...
Metabolomics Studies in Cushing's Syndrome: Recent Developments and Perspectives [0.03%]
库欣综合征代谢组学研究的新进展及展望
Sowrabha Bhat,Vanya Kadla Narayana,T S Keshava Prasad
Sowrabha Bhat
Introduction: Exogenous Cushing's syndrome is the result of long-term exposure to glucocorticoids, while endogenous Cushing's syndrome occurs due to excessive production of glucocorticoids within the body. Cushing's syndr...
T cell population size control by coronin 1 uncovered: from a spot identified by two-dimensional gel electrophoresis to quantitative proteomics [0.03%]
经二维电泳发现的CORONIN 1调控T细胞群体大小：从点到定量蛋白质组学
Tohnyui Fabrice Ndinyanka,Katarzyna Buczak,Alexander Schmidt et al.
Tohnyui Fabrice Ndinyanka et al.
Introduction: Recent work identified members of the evolutionarily conserved coronin protein family as key regulators of cell population size. This work originated ~25 years ago through the identification, by two-dimensio...
AlphaFold and what is next: bridging functional, systems and structural biology [0.03%]
AlphaFold及未来展望——架起功能、系统与结构生物学之间的桥梁
Kacper Szczepski,Lukasz Jaremko
Kacper Szczepski
Introduction: The DeepMind's AlphaFold (AF) has revolutionized biomedical research by providing both experts and non-experts with an invaluable tool for predicting protein structures. However, while AF is highly effective...
Oncoproteins E6/E7 of the human papillomavirus types 16 & 18 synergize in modulating oncogenes and tumor suppressor proteins in colorectal cancer [0.03%]
人乳头瘤病毒16/18型癌蛋白E6/E7在结直肠癌中协同调节癌基因和抑癌蛋白
Queenie Fernandes,Lubna Therachiyil,Shahd M Younis et al.
Queenie Fernandes et al.
Objective: Our study presents a novel analysis of the oncogenes and tumor suppressor proteins directly modulated by E6/E7 of high-risk HPV types 16 and 18, in colorectal cancer (CRC). ...
Interaction and regulation of the mitochondrial proteome - in health and disease [0.03%]
线粒体蛋白质组的相互作用和调节：健康与疾病中的作用
Johan Palmfeldt
Johan Palmfeldt
Introduction: Mitochondria contain multiple pathways including energy metabolism and several signaling and synthetic pathways. Mitochondrial proteomics is highly valuable for studying diseases including inherited metaboli...