Multi-cohort, cross-species urinary proteomics reveals signatures of LRRK2 dysfunction in Parkinson's disease [0.03%]
多队列、跨物种尿蛋白组学揭示了帕金森病中LRRK2功能障碍的标志物
Duc Tung Vu,William Sibran,Andreas Metousis et al.
Duc Tung Vu et al.
Pathogenic mutations in Leucine-rich repeat kinase 2 (LRRK2) are the predominant genetic cause of Parkinson's disease (PD) and often increase kinase activity, making LRRK2 inhibitors promising treatment options. Although LRRK2 kinase inhibi...
Szilvia Juhász,Benjamin Tamás Papp,Anna Tácia Fülöp et al.
Szilvia Juhász et al.
Although numerous mutational processes operate in cancer, their functional impacts are unclear. We hypothesised that certain mutation sources preferentially generate amino acid substitutions that evade immune recognition, producing immune-c...
Protein buffering of aneuploidy is driven by coordinated factors identified through machine learning [0.03%]
机器学习驱动揭示蛋白缓冲染色体非整倍性通过整体因子协调实现
Erik Marcel Heller,Karen Barthel,Markus Räschle et al.
Erik Marcel Heller et al.
Aneuploidy, a hallmark of cancer, alters chromosome copy numbers and with that the abundance of hundreds of proteins. Evidence suggests that levels of proteins encoded on affected chromosomes are often buffered toward their abundances obser...
Essentiality and dynamic expression of the human tRNA pool during viral infection [0.03%]
人类病毒感染期间tRNA池的必需性及其动态表达
Noa Aharon-Hefetz,Michal Schwartz,Einav Aharon et al.
Noa Aharon-Hefetz et al.
Human viruses rely on host translation resources, including the cellular tRNA pool, because they lack tRNA genes. Using tRNA sequencing, we profiled mature tRNAs during infections with human cytomegalovirus (HCMV) and SARS-CoV-2. HCMV-induc...
Predicting the protein interaction landscape of a free-living bacterium with pooled-AlphaFold3 [0.03%]
基于整合AlphaFold3预测的大肠杆菌蛋白质相互作用图谱
Horia Todor,Lili M Kim,Jürgen Jänes et al.
Horia Todor et al.
Accurate prediction of protein complex structures by AlphaFold3 and similar programs has been used to predict the presence of protein-protein interactions (PPIs), but this technique has never been applied to an entire genome due to onerous ...
The DNA dialect: a comprehensive guide to pretrained genomic language models [0.03%]
DNA方言：预训练基因组语言模型的全面指南
Marcell Veiner,Fran Supek
Marcell Veiner
Following their success in natural language processing and protein biology, pretrained large language models have started appearing in genomics in large numbers. These genomic language models (gLMs), trained on diverse DNA and RNA sequences...
Pooled single-cell screen in colorectal cancer defines transcriptional modules linked to oncogenes [0.03%]
pooled单细胞筛选定义了与癌基因相关的转录模块在结直肠癌中
Viola Hollek,Francisca Böhning,Catalina Florez Vargas et al.
Viola Hollek et al.
Oncogenic mutations shape colorectal cancer (CRC) biology, yet their impact on transcriptional phenotypes remains incompletely understood, and their individual prognostic value is limited. Here, we perform a pooled single-cell transcriptomi...
Chromosome length is constrained by spindle scaling to ensure faithful mitosis in mammals [0.03%]
染色体长度通过纺锤体放缩机制受到限制以保证哺乳动物有丝分裂的忠实性
Yu-Long Zhao,Yi-Ming Zhao,Yi-Fang Yan et al.
Yu-Long Zhao et al.
Why eukaryotic genomes are universally divided among multiple chromosomes remains an unresolved question. Although yeast and mouse cells can tolerate chromosomal fusions without impairing viability, we show here that chromosome length in ma...
Unlocking CRISPR-Cas9 editing for widely diverse Dictyostelid species [0.03%]
揭秘多样化土壤生物Dictyostelium的CRISPR-Cas9编辑技术
Mireia Garriga-Canut,Nikki Cannon,Matt Benton et al.
Mireia Garriga-Canut et al.
Dictyostelids are a species-rich clade of cellular slime molds that are widely found in soils and have been studied for over a century. Due to a lack of genome editing methods, most molecular research in Dictyostelids has focused on only a ...
Limited proteolysis-coupled mass spectrometry captures proteome-wide protein structural alterations and biomolecular condensation in living cells [0.03%]
利用限制性蛋白酶解结合质谱法在活细胞中捕获蛋白质组范围内的蛋白质结构变化及生物分子凝聚体形成
Franziska Elsässer,Roberta Florea,Felix Räsch et al.
Franziska Elsässer et al.
The function of a protein is determined by its structure, which may change dynamically in response to post-translational modifications, interaction with other molecules, or environmental factors like temperature. Limited proteolysis-coupled...