Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SpyCEP, a candidate antigen for a vaccine against Streptococcus pyogenes [0.03%]
一种克隆、表达、纯化、结晶及SpyCEP的初步X射线衍射分析(SpyCEP是一种抗链球菌素疫苗候选抗原)
Francesca Abate,Enrico Malito,Fabiana Falugi et al.
Francesca Abate et al.
Streptococcus pyogenes (Group A streptococcus; GAS) is an important human pathogen against which an effective vaccine does not yet exist. The S. pyogenes protein SpyCEP (S. pyogenes cell-envelope proteinase) is a surface-exposed subtilisin-...
Preliminary X-ray diffraction analysis of thermostable β-1,4-mannanase from Aspergillus niger BK01 [0.03%]
来源于Aspergillus niger BK01的耐热β-1,4-甘露聚糖酶的初步X射线衍射分析
Wenhua Luo,Jian-Wen Huang,Chun-Hsiang Huang et al.
Wenhua Luo et al.
β-1,4-Mannanase (β-mannanase) is a key enzyme in decomposing mannans, which are abundant components of hemicelluloses in the plant cell wall. Therefore, mannan hydrolysis is highly valuable in a wide array of industrial applications. β-M...
Expression, purification and crystallization of the FP domain of the human F-box protein Fbxo7 [0.03%]
人类F-box蛋白FBXO7的FP结构域的表达、纯化与结晶
Jinsai Shang,Guan Wang,Yang Yang et al.
Jinsai Shang et al.
Fbxo7 is a conserved protein in higher eukaryotes that belongs to the F-box protein family. Fbxo7 is the substrate-recognition component of the SCFFbxo7 (Skp1-Cul1-Fbox protein) E3 ubiquitin ligase. Besides the F-box motif, Fbxo7 also conta...
Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1 [0.03%]
亚麻细胞分裂素氧化酶LuCKX1.1的结晶及初步X射线衍射分析
Li Wan,Simon J Williams,Xiaoxiao Zhang et al.
Li Wan et al.
The plant hormones cytokinins play a central role in regulating cell division and developmental events. Cytokinin oxidase regulates the levels of these plant hormones by catalyzing their irreversible oxidation, which contributes to the regu...
Structure of succinyl-CoA:3-ketoacid CoA transferase from Drosophila melanogaster [0.03%]
黑腹果蝇琥珀酰辅酶A：3-酮酸辅酶A转移酶的结构
Min Zhang,Han-Yang Xu,Yi-Cui Wang et al.
Min Zhang et al.
Succinyl-CoA:3-ketoacid CoA transferase (SCOT) plays a crucial role in ketone-body metabolism. SCOT from Drosophila melanogaster (DmSCOT) was purified and crystallized. The crystal structure of DmSCOT was determined at 2.64 Å resolution an...
Structure of 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa [0.03%]
铜绿假单胞菌2-酮-3-脱氧-D-.manuo辛二磷酸合成酶的结构
Sarah K Nelson,Alan Kelleher,Gonteria Robinson et al.
Sarah K Nelson et al.
Pseudomonas aeruginosa is a major cause of opportunistic infection and is resistant to most antibiotics. As part of efforts to generate much-needed new antibiotics, structural studies of enzymes that are critical for the virulence of P. aer...
Nicholas A Zeringo,Lea Murphy,Eric A McCloskey et al.
Nicholas A Zeringo et al.
Casein kinase 1 δ (CK1δ) is a regulatory enzyme in the mammalian circadian oscillator and represents a potential pharmacological target for modulating circadian rhythms. Crystal structures of four different polymorphs of CK1δ have previo...
Unexpected features in the Protein Data Bank entries 3qd1 and 4i8e: the structural description of the binding of the serine-rich repeat adhesin GspB to host cell carbohydrate receptor is not a solved issue [0.03%]
蛋白数据库条目3qd1和4i8e中的意外特征：关于丝氨酸丰富重复粘附素GspB与宿主细胞碳水化合物受体结合的结构描述仍存在未解决问题
Yves A Muller
Yves A Muller
The structure of a complex between a fragment of the adhesin GspB from Streptococcus gordonii and a disaccharide (PDB entries 3qd1 and 4i8e) has recently been proposed to identify the binding site for the sialyl-T antigen recognized by GspB...
Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser [0.03%]
在环境温度下使用X射线自由电子激光对悬浮于液滴中的30S核糖体亚基微晶体进行序列飞秒X射线衍射研究
Hasan Demirci,Raymond G Sierra,Hartawan Laksmono et al.
Hasan Demirci et al.
High-resolution ribosome structures determined by X-ray crystallography have provided important insights into the mechanism of translation. Such studies have thus far relied on large ribosome crystals kept at cryogenic temperatures to reduc...
Identifying ligand-binding hot spots in proteins using brominated fragments [0.03%]
利用溴化片段识别蛋白质配体结合的热点区域
Morten K Grøftehauge,Martin Ø Therkelsen,Rolf Taaning et al.
Morten K Grøftehauge et al.
High-quality crystals of Thermus thermophilus EF-Tu in the GTP-bound conformation at 1.7-2.7 Å resolution were used to test 18 small organic molecules, all brominated for confident identification in the anomalous difference maps. From this...