Overexpression, crystallization and preliminary X-ray crystallographic analysis of glucuronoxylan xylanohydrolase (Xyn30A) from Clostridium thermocellum [0.03%]
热解纤维杆菌葡萄糖醛酸木聚糖木聚糖水解酶(Xyn30A)的过表达、结晶和初步X射线衍射分析
Anil Kumar Verma,Arun Goyal,Filipe Freire et al.
Anil Kumar Verma et al.
The modular carbohydrate-active enzyme belonging to glycoside hydrolase family 30 (GH30) from Clostridium thermocellum (CtXynGH30) is a cellulosomal protein which plays an important role in plant cell-wall degradation. The full-length CtXyn...
Crystallization and preliminary X-ray diffraction analysis of D53H mutant Escherichia coli cAMP receptor protein [0.03%]
D53H突变的大肠杆菌cAMP受体蛋白的结晶及初步X射线衍射分析
Jing Huang,Tong Wu,Zheng Guo et al.
Jing Huang et al.
The Escherichia coli cyclic AMP receptor protein (CRP) is a prokaryotic global transcription activator protein that controls the expression of many different genes. Wild-type CRP can bind to special DNA sequences in the presence of cAMP. Th...
Purification, crystallization and preliminary crystallographic analysis of the ligand-binding regions of the PctA and PctB chemoreceptors from Pseudomonas aeruginosa in complex with amino acids [0.03%]
铜绿假单胞菌中与氨基酸结合的化学感受器PctA和PctB配体结合区的纯化、结晶及初步晶体学分析
Miriam Rico-Jiménez,Francisco Muñoz-Martínez,Tino Krell et al.
Miriam Rico-Jiménez et al.
Pseudomonas aeruginosa is an opportunistic pathogen and one of the major model organisms for the study of chemotaxis. The bacterium harbours 26 genes encoding chemoreceptors, most of which have not been annotated with a function. The paralo...
Expression, purification, crystallization and preliminary X-ray diffraction analysis of the aspartate transcarbamoylase domain of human CAD [0.03%]
人CAD天冬氨酸转运碳氨酶结构域的表达、纯化、结晶及初步X射线衍射分析
Alba Ruiz-Ramos,Nada Lallous,Araceli Grande-García et al.
Alba Ruiz-Ramos et al.
Aspartate transcarbamoylase (ATCase) catalyzes the synthesis of N-carbamoyl-L-aspartate from carbamoyl phosphate and aspartate in the second step of the de novo biosynthesis of pyrimidines. In prokaryotes, the first three activities of the ...
Cloning, expression, purification, crystallization and preliminary X-ray studies of argininosuccinate lyase (Rv1659) from Mycobacterium tuberculosis [0.03%]
结核分支杆菌鸟氨酸琥珀酸裂解酶（Rv1659）的克隆、表达、纯化、结晶和初步X射线研究
A Paul,A Mishra,A Surolia et al.
A Paul et al.
The last enzyme in the arginine-biosynthesis pathway, argininosuccinate lyase, from Mycobacterium tuberculosis has been cloned, expressed, purified and crystallized, and preliminary X-ray studies have been carried out on the crystals. The H...
Expression, purification, crystallization and preliminary X-ray analysis of the receiver domain of Staphylococcus aureus LytR protein [0.03%]
金黄色葡萄球菌LytR蛋白接收域的表达、纯化、结晶及初步X射线分析
Agnesa Shala,Kevin H Patel,Dasantila Golemi-Kotra et al.
Agnesa Shala et al.
The response-regulatory protein LytR belongs to a family of transcription factors involved in the regulation of important virulence factors in pathogenic bacteria. The protein consists of a receiver domain and an effector domain, which play...
Crystallization and preliminary X-ray diffraction studies of the (R)-selective amine transaminase from Aspergillus fumigatus [0.03%]
来自烟曲霉的(R)型选择性胺转氨酶的结晶及初步X射线衍射研究
Maren Thomsen,Lilly Skalden,Gottfried J Palm et al.
Maren Thomsen et al.
The (R)-selective amine transaminase from Aspergillus fumigatus was expressed in Escherichia coli and purified to homogeneity. Bright yellow crystals appeared while storing the concentrated solution in the refrigerator and belonged to space...
Crystallization and preliminary X-ray crystallographic analysis of the inhibitory domain of the tomato mosaic virus resistance protein Tm-1 [0.03%]
番茄条纹病毒抗性蛋白Tm-1抑制结构域的结晶及初步X射线衍射分析
Masahiko Kato,Yuichiro Kezuka,Chihoko Kobayashi et al.
Masahiko Kato et al.
Tm-1, an inhibitor protein of Tomato mosaic virus RNA replication, contains two conserved domains: an uncharacterized domain at its N-terminus and a TIM-barrel-like domain at its C-terminus. The N-terminal domain of Tm-1 has an inhibitory a...
Liquid-liquid diffusion crystallization improves the X-ray diffraction of EndoS, an endo-β-N-acetylglucosaminidase from Streptococcus pyogenes with activity on human IgG [0.03%]
液滴扩散结晶法改善了人IgG的链球菌EndoS（一种内切-β-N-乙酰氨基己糖苷酶）的X射线衍射性能
Beatriz Trastoy,Joseph V Lomino,Lai Xi Wang et al.
Beatriz Trastoy et al.
Endoglycosidase S (EndoS) is an enzyme secreted by Streptococcus pyogenes that specifically hydrolyzes the β-1,4-di-N-acetylchitobiose core glycan on immunoglobulin G (IgG) antibodies. One of the most common human pathogens and the cause o...
Preliminary crystallographic studies of BRCA1 BRCT-ABRAXAS complex [0.03%]
BRCA1 BRCT-ABRAXAS复合物的初步晶体学研究
Dilip C Badgujar,Ulka Sawant;Vikrant;Lumbini Yadav,M V Hosur et al.
Dilip C Badgujar et al.
The BRCA1 holoenzyme complex plays an important role in DNA damage repair. ABRAXAS is a newly discovered component of this complex and its C-terminal region directly binds to the BRCA1 BRCT domain. Single crystals of the BRCA1 BRCT-ABRAXAS ...