Crystallization and preliminary X-ray diffraction analysis of a high-affinity phosphate-binding protein endowed with phosphatase activity from Pseudomonas aeruginosa PAO1 [0.03%]
来源于绿脓杆菌PA01的高亲和力磷酸盐结合蛋白的结晶及初步X射线衍射分析研究
Ahmed Djeghader,Guillaume Gotthard,Andrew Suh et al.
Ahmed Djeghader et al.
In prokaryotes, phosphate starvation induces the expression of numerous phosphate-responsive genes, such as the pst operon including the high-affinity phosphate-binding protein (PBP or pstS) and alkaline phosphatases such as PhoA. This resp...
Crystallization and preliminary X-ray study of a (2R,3R)-2,3-butanediol dehydrogenase from Bacillus coagulans 2-6 [0.03%]
来源于凝固芽孢杆菌2-6的(2R,3R)-2,3-丁二醇脱氢酶的结晶及其初步X射线衍射研究
Xiangzhi Miao,Xianhui Huang,Guofang Zhang et al.
Xiangzhi Miao et al.
(2R,3R)-2,3-Butanediol dehydrogenase (R,R-BDH) from Bacillus coagulans 2-6 is a zinc-dependent medium-chain alcohol dehydrogenase. Recombinant R,R-BDH with a His6 tag at the C-terminus was expressed in Escherichia coli BL21 (DE3) cells and ...
Crystallization of the C-terminal head domain of the fibre protein from a siadenovirus, turkey adenovirus 3 [0.03%]
鸡腺病毒3型纤突蛋白C端头结构域的晶体结构
Abhimanyu K Singh,Mónika Z Ballmann,Mária Benkő et al.
Abhimanyu K Singh et al.
Turkey adenovirus 3 belongs to the genus Siadenovirus. Its predicted fibre protein consists of an N-terminal virus-attachment domain, a central shaft domain and a head domain at the C-terminus. The head domain has little sequence identity t...
Crystallization and preliminary X-ray diffraction studies of D-threo-3-hydroxyaspartate dehydratase isolated from Delftia sp. HT23 [0.03%]
D-苏型-3-羟基天门冬氨酸脱水酶的结晶及初步X射线衍射研究（来源于Delftia菌HT23）
Yu Matsumoto,Yoshiaki Yasutake,Yuki Takeda et al.
Yu Matsumoto et al.
D-threo-3-Hydroxyaspartate dehydratase (D-THA DH) isolated from the soil bacterium Delftia sp. HT23 is a novel enzyme consisting of 380 amino-acid residues which catalyzes the conversion of D-threo-3-hydroxyaspartate to oxaloacetate and amm...
Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron [0.03%]
来自Bacteroides thetaiotaomicron的假定木糖异构酶的过表达、结晶和初步X射线晶体学分析
Jea-Won Cho,Byeong-Gu Han,Sang Youn Park et al.
Jea-Won Cho et al.
Bacteroides thetaiotaomicron BT0793, a putative xylose isomerase, was overexpressed in Escherichia coli, purified and crystallized using polyethylene glycol monomethyl ether 550 as the precipitant. X-ray diffraction data were collected to 2...
Identification, characterization and preliminary X-ray diffraction analysis of the rolling-circle replication initiator protein from plasmid pSTK1 [0.03%]
来自于质粒pSTK1的滚环复制起始蛋白的鉴定、表征及初步X-射线衍射分析
Stephen B Carr,Lauren B Mecia,Simon E V Phillips et al.
Stephen B Carr et al.
Antibiotic resistance in bacterial pathogens poses an ever-increasing risk to human health. In antibiotic-resistant strains of Staphylococcus aureus this resistance often resides in extra-chromosomal plasmids, such as those of the pT181 fam...
Expression, crystallization and preliminary X-ray crystallographic analysis of peptide deformylase from Campylobacter jejuni [0.03%]
来自Campylobacterjejuni的肽脱甲酰酶的表达、结晶及初步的X射线晶体学分析
Huyen Thi Tran,Tan-Viet Pham,Ho-Phuong-Thuy Ngo et al.
Huyen Thi Tran et al.
Campylobacter jejuni is one of the major foodborne pathogens causing human infection. Peptide deformylase, a metallohydrolase, catalyzes the deformylation of N-formylated methionine in newly synthesized polypeptides in prokaryotes and some ...
Crystallization and preliminary crystallographic analysis of GanB, a GH42 intracellular β-galactosidase from Geobacillus stearothermophilus [0.03%]
嗜热解糖假芽孢杆菌GH42型胞内β-半乳糖苷酶GanB的结晶及初步晶体学分析
Hodaya V Solomon,Orly Tabachnikov,Hadar Feinberg et al.
Hodaya V Solomon et al.
Geobacillus stearothermophilus T-6 is a Gram-positive thermophilic soil bacterium that contains a multi-enzyme system for the utilization of plant cell-wall polysaccharides, including xylan, arabinan and galactan. The bacterium uses a numbe...
Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of Rv0241c (HtdX) from Mycobacterium tuberculosis H37Rv [0.03%]
结核杆菌Rv0241c (HtdX)的克隆、过量表达、纯化、结晶和初步X射线衍射分析
Rupam Biswas,Debajyoti Dutta,Amit Kumar Das
Rupam Biswas
Rv0241c (HtdX) is a putative (3R)-hydroxyacyl-CoA dehydratase of Mycobacterium tuberculosis. The htdX gene belongs to a conserved operon and is expressed in mycobacteria in the presence of several fatty-acid synthase II drugs. To elucidate ...
Purification, crystallization and preliminary crystallographic studies of C-terminal RNA recognition motif (RRM-3) of human ELAV-type RNA-binding protein 3 (ETR-3) [0.03%]
人ETR-3蛋白质C端RNA识别模体（RRM-3）的纯化、结晶及初步晶体学研究
Maruthi Kashyap,Ashwani Sharma,Neel Sarovar Bhavesh
Maruthi Kashyap
Human embryonically lethal abnormal vision (ELAV)-type RNA-binding protein 3 (ETR-3) has been implicated in many aspects of RNA-processing events including alternative splicing, stability, editing and translation. RNA recognition motif 3 (R...