Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant α-cyclodextrin glucanotransferase from Bacillus macerans [0.03%]
来自Bacillus macerans的Tyr167His突变α-环糊精葡萄糖转移酶的结晶及初步X射线衍射研究
Yang Yue,Shengquan Liu,Hongbin Li et al.
Yang Yue et al.
Improving the specificity of α-cyclodextrin glucanotransferase is a significant issue in the field of α-cyclodextrin production. In this study, a constructed Y167H mutant α-cyclodextrin glucanotransferase with enhanced α-cyclodextrin sp...
Purification, crystallization and preliminary crystallographic analysis of the SpaA backbone-pilin subunit from probiotic Lactobacillus rhamnosus GG [0.03%]
来自益生菌鼠李糖乳杆菌GG的SpaA骨干纤毛蛋白亚基的纯化、结晶及初步晶体学分析
Deepak Singh,Ingemar von Ossowski,Airi Palva et al.
Deepak Singh et al.
Lactobacillus rhamnosus GG, a widely used Gram-positive probiotic strain, is clinically well known for its perceived health-promoting effects. It has recently been shown to display proteinaceous pilus fibres (called SpaCBA) on its cell surf...
Cloning to crystallization of dihydrodipicolinate synthase from the intracellular pathogen Legionella pneumophila [0.03%]
克隆、结晶并测定Legionella pneumophila胞内致病菌二氢苯并噁嗪𬭩合成酶的结构
Tanzeela Siddiqui,Jason J Paxman,Con Dogovski et al.
Tanzeela Siddiqui et al.
Dihydrodipicolinate synthase (DHDPS) catalyses the rate-limiting step in the biosynthesis of meso-diaminopimelate and lysine. Here, the cloning, expression, purification and crystallization of DHDPS from the intracellular pathogen Legionell...
Crystallization and preliminary structural characterization of the two actin isoforms of the malaria parasite [0.03%]
疟原虫两种肌动蛋白异构体的结晶及初步结构特征分析
Saligram Prabhakar Bhargav,Juha Vahokoski,Esa-Pekka Kumpula et al.
Saligram Prabhakar Bhargav et al.
Malaria is a devastating disease caused by apicomplexan parasites of the genus Plasmodium that use a divergent actin-powered molecular motor for motility and invasion. Plasmodium actin differs from canonical actins in sequence, structure an...
Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of the TIR domain from the Brucella melitensis TIR-domain-containing protein TcpB [0.03%]
米氏布鲁杆菌TcpB蛋白的Toll/白介素-1受体（TIR）结构域的克隆、表达、纯化、结晶及初步X射线晶体学分析
Mohammed Alaidarous,Thomas Ve,M Obayed Ullah et al.
Mohammed Alaidarous et al.
In mammals, Toll-like receptors (TLRs) recognize conserved microbial molecular signatures and induce an early innate immune response in the host. TLR signalling is mediated by interactions between the cytosolic TIR (Toll/interleukin-1 recep...
Expression, crystallization and preliminary X-ray crystallographic analysis of cellobiose 2-epimerase from Dictyoglomus turgidum DSM 6724 [0.03%]
来自Dictyoglomus turgidum DSM 6724的半乳糖苷二酸酶的表达、结晶及其初步X射线晶体学分析
Tan-Viet Pham,Seung-Hye Hong,Myoung-ki Hong et al.
Tan-Viet Pham et al.
Cellobiose 2-epimerase epimerizes and isomerizes β-1,4- and α-1,4-gluco-oligosaccharides. N-Acyl-D-glucosamine 2-epimerase (DT_epimerase) from Dictyoglomus turgidum has an unusually high catalytic activity towards its substrate cellobiose...
Purification, crystallization and preliminary X-ray crystallographic analysis of the transport unit of the monomeric autotransporter AIDA-I from Escherichia coli [0.03%]
大肠杆菌单聚体自转运蛋白AIDA-I的运输单位的纯化、结晶及初步X射线晶体学分析
Iris Gawarzewski,Britta Tschapek,Astrid Hoeppner et al.
Iris Gawarzewski et al.
The adhesin involved in diffuse adherence (AIDA-I) from Escherichia coli belongs to the group of autotransporters, specifically the type Va secretion system (T5aSS). All autotransporter systems contain a C-terminal β-domain, which forms a ...
Expression, purification and preliminary X-ray crystallographic analysis of Arf1-GDP in complex with dimeric p23 peptide [0.03%]
Arf1-GDP与二聚体p23肽复合物的表达、纯化及初步X射线晶体学分析
Peng Zheng,Feng Gao,Kai Deng et al.
Peng Zheng et al.
Arf1 is a member of the Ras superfamily and is involved in COPI vesicle formation. Arf1-GDP can interact with dimeric p23. Here, human Arf1 (residues 18-181) was cloned, expressed and purified in Escherichia coli. For crystallization, Arf1-...
Crystallization and X-ray diffraction analysis of nylon hydrolase (NylC) from Arthrobacter sp. KI72 [0.03%]
来自Arthrobacter sp. KI72的尼龙水解酶(NylC)的结晶和X射线衍射分析
Keisuke Nagai,Kengo Yasuhira,Yusuke Tanaka et al.
Keisuke Nagai et al.
Nylon hydrolase (NylC) encoded by Arthrobacter plasmid pOAD2 (NylCp2) was expressed in Escherichia coli JM109 and purified by ammonium sulfate fractionation, anion-exchange column chromatography and gel-filtration chromatography. NylCp2 was...
Crystallization and preliminary crystallographic analysis of a viral RNA-silencing suppressor encoded by Wuhan nodavirus [0.03%]
武汉鱼类结节病毒RNA干扰抑制物的结晶与初步晶体学分析
Yuanyuan Liu,Yuhui Zhao,Yong Zhang et al.
Yuanyuan Liu et al.
Wuhan nodavirus (WhNV), which is a new member of the Nodaviridae family, encodes a viral protein, B2, that suppresses RNA silencing and host-cell RNA interference (RNAi)-mediated immunity. Although Flock House virus (FHV), another member of...