Crystallization and preliminary X-ray diffraction of the RNA demethylase ALKBH5 [0.03%]
ALKBH5去甲基酶的结晶及初步X射线衍射分析
Bin Zhou,Zhifu Han
Bin Zhou
N(6)-methyladenosine (m6A) is a ubiquitous modification found in mammalian mRNA and long noncoding RNA. ALKBH5 is a member of the iron(II)- and 2-oxoglutarate-dependent AlkB oxygenase family and has been shown to catalyze the oxidative deme...
Crystallization and preliminary X-ray study of alanine dehydrogenase from Bacillus pseudofirmus OF4 [0.03%]
来自Bacillus pseudofirmus OF4的丙氨酸脱氢酶的结晶及初步X射线研究
Jinjin Wen,Zhenzhen Li,Guangzheng He et al.
Jinjin Wen et al.
Alanine dehydrogenase (OF4Ald) from the alkaliphilic Bacillus pseudofirmus OF4 was expressed and purified with a His6 tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion metho...
GPX3 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis [0.03%]
拟南芥GPX3基因的克隆、表达、纯化、结晶及初步衍射分析
Kun Li,Qingzhan Yang,Wei Wang et al.
Kun Li et al.
The Arabidopsis thaliana glutathione peroxidase 3 (GPX3) gene encodes a glutathione peroxidase with roles in H2O2 homeostasis and signalling. The GPX3 gene sequence was cloned into pGEX-6P1 and overexpressed in Escherichia coli. The GPX3 pr...
Purification, crystallization and preliminary crystallographic analysis of the globular domain of the human type V myosin Myo5a [0.03%]
人类Myo5a肌球蛋白V型球状结构域的纯化、结晶及初步晶体学分析
Hana Velvarska,Dierk Niessing
Hana Velvarska
Type V myosins constitute the main cargo-transporting class of myosin motors in higher eukaryotes. They are mainly defined by their C-terminal globular domain, which is required for cargo binding as well as for motor auto-inhibition in the ...
Crystallization and preliminary X-ray diffraction studies of Staphylococcus aureus homoserine dehydrogenase [0.03%]
金黄色葡萄球菌同丝氨酸脱氢酶的结晶及初步X射线衍射研究
Vikas Navratna,Balasubramanian Gopal
Vikas Navratna
Staphylococcus aureus is a Gram-positive nosocomial pathogen. The prevalence of multidrug-resistant S. aureus strains in both hospital and community settings makes it imperative to characterize new drug targets to combat S. aureus infection...
Crystallization and X-ray crystallographic analysis of the cholesterol-dependent cytolysin listeriolysin O from Listeria monocytogenes [0.03%]
李斯特菌溶血素O的结晶及X射线晶体学分析
Stefan Köster,Martina Hudel,Trinad Chakraborty et al.
Stefan Köster et al.
The secreted pore-forming toxin listeriolysin O (LLO) from the intracellular pathogen Listeria monocytogenes is a member of the family of cholesterol-dependent cytolysins (CDC) with broad properties in pathogenesis. Its role as a virulence ...
A family portrait: structural comparison of the Whirly proteins from Arabidopsis thaliana and Solanum tuberosum [0.03%]
拟南芥和马铃薯WHIRLY家族成员结构比较研究
Laurent Cappadocia,Jean-Sébastien Parent,Jurgen Sygusch et al.
Laurent Cappadocia et al.
DNA double-strand breaks are highly detrimental genomic lesions that routinely arise in genomes. To protect the integrity of their genetic information, all organisms have evolved specialized DNA-repair mechanisms. Whirly proteins modulate D...
The tertiary structure of an i-type lysozyme isolated from the common orient clam (Meretrix lusoria) [0.03%]
来自普通东方帘蛤（Meretrix lusoria）的i型溶菌酶三级结构
Yuko Kuwano,Kazunari Yoneda,Yuya Kawaguchi et al.
Yuko Kuwano et al.
To evaluate the structure-function relationships of invertebrate lysozymes, a new invertebrate-type (i-type) lysozyme was isolated from the common orient clam (Meretrix lusoria) and the tertiary structure of this enzyme was determined. Comp...
Structure of CT584 from Chlamydia trachomatis refined to 3.05 Å resolution [0.03%]
衣原体CT584蛋白的三维结构——3.05埃分辨率下的同源模型法计算模拟结构
Michael L Barta,John Hickey,Kyle E Kemege et al.
Michael L Barta et al.
Chlamydia trachomatis is a major cause of various diseases, including blinding trachoma and pelvic inflammatory disease, and is the leading reported sexually transmitted bacterial infection worldwide. All pathogenic Chlamydiae spp. utilize ...
Structures of Saccharomyces cerevisiae D-arabinose dehydrogenase Ara1 and its complex with NADPH: implications for cofactor-assisted substrate recognition [0.03%]
酿酒酵母D-阿拉伯糖脱氢酶Ara1及其与NADPH复合物的结构：辅因子辅助底物识别的意义
Xiao-Qian Hu,Peng-Chao Guo,Jin-Di Ma et al.
Xiao-Qian Hu et al.
The primary role of yeast Ara1, previously mis-annotated as a D-arabinose dehydrogenase, is to catalyze the reduction of a variety of toxic α,β-dicarbonyl compounds using NADPH as a cofactor at physiological pH levels. Here, crystal struc...