Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa [0.03%]
产金属β内酰胺酶IMP-18的纯化、结晶及初步X射线衍射分析
Takamitsu Furuyama,Yoshikazu Ishii,Norimasa Ohya et al.
Takamitsu Furuyama et al.
Class B β-lactamases are known as metallo-β-lactamases (MBLs) and they hydrolyze most β-lactams, including carbapenems. IMP-18, an MBL cloned from Pseudomonas aeruginosa, was overexpressed, purified and crystallized by vapour diffusion f...
Crystallization of the CHAP domain of the endolysin from Staphylococcus aureus bacteriophage K [0.03%]
来自金黄色葡萄球菌噬菌体K的胞内酶CHAP结构域结晶
Marta Sanz-Gaitero,Ruth Keary,Carmela Garcia-Doval et al.
Marta Sanz-Gaitero et al.
CHAP(K) is the N-terminal cysteine, histidine-dependent amidohydrolase/peptidase domain (CHAP domain) of the Staphylococcus aureus bacteriophage K endolysin LysK. It is formed from the first 165 residues of LysK and functions by cleaving sp...
Crystallization and sulfur SAD phasing of AggA, the major subunit of aggregative adherence fimbriae type I from the Escherichia coli strain that caused an outbreak of haemolytic-uraemic syndrome in Germany [0.03%]
德国产志贺毒素的大肠杆菌菌株中聚集粘附性菌毛I型主要亚基AggA的结晶和SAD解析
Natalia Pakharukova,Minna Tuittila,Anton Zavialov
Natalia Pakharukova
The outbreak of Shiga toxin-producing Escherichia coli O104:H4 infection in Germany in 2011 was associated with significant mortality and morbidity owing to the progressive development of haemolytic-uraemic syndrome. The outbreak strain eme...
Crystallization and preliminary X-ray analysis of the ATPase domain of the σ(54)-dependent transcription activator NtrC1 from Aquifex aeolicus bound to the ATP analog ADP-BeFx [0.03%]
结合ADP-BeFx的σ(54)依赖型转录激活因子NtrC1的大肠杆菌ATP酶结构域的结晶和初步X射线分析
Tatyana A Sysoeva,Neela Yennawar,Marc Allaire et al.
Tatyana A Sysoeva et al.
One way that bacteria regulate the transcription of specific genes to adapt to environmental challenges is to use different σ factors that direct the RNA polymerase holoenzyme to distinct promoters. Unlike σ(70) RNA polymerase (RNAP), σ(...
Crystallization and preliminary crystallographic analysis of a surface antigen glycoprotein, SAG19, from Eimeria tenella [0.03%]
艾美尔十号球虫表面抗原糖蛋白SAG19的结晶及初步晶体学分析
Nur Zazarina Ramly,Sergey N Rouzheinikov,Svetlana E Sedelnikova et al.
Nur Zazarina Ramly et al.
Coccidiosis in chickens is caused by the apicomplexan parasite Eimeria tenella and is thought to involve a role for a superfamily of more than 20 cysteine-rich surface antigen glycoproteins (SAGs) in host-parasite interactions. A representa...
Crystallization of the C-terminal domain of the fibre protein from snake adenovirus 1, an atadenovirus [0.03%]
蛇腺病毒1型纤丝蛋白C末端结构域的结晶初探——一种At腺病毒
Abhimanyu K Singh,Rosa Menéndez-Conejero,Carmen San Martín et al.
Abhimanyu K Singh et al.
Adenovirus fibre proteins play an important role in determining viral tropism. The C-terminal domain of the fibre protein from snake adenovirus type 1, a member of the Atadenovirus genus, has been expressed, purified and crystallized. Cryst...
Crystallization and preliminary crystallographic analysis of the chimeric protein LKB1-14-3-3ζ [0.03%]
LKB1-14-3-3ζ嵌合蛋白的结晶及初步晶体学分析
Sheng Ding,Ruiqing Zhou,Yaqin Zhu
Sheng Ding
LKB1, a tumour suppressor, plays key roles in cell polarity, cell growth and energy metabolism. 14-3-3 proteins bind to LKB1 and suppress its functions. A chimera containing 14-3-3ζ and its binding region in LKB1 was constructed and the ch...
Two-dimensional crystallization of intact F-ATP synthase isolated from bovine heart mitochondria [0.03%]
来自牛心脏线粒体的完整F-ATP合酶的二维结晶
Shintaro Maeda,Kyoko Shinzawa-Itoh,Kaoru Mieda et al.
Shintaro Maeda et al.
Mitochondrial F-ATP synthase produces the majority of ATP for cellular functions requiring free energy. The structural basis for proton motive force-driven rotational catalysis of ATP formation in the holoenzyme remains to be determined. He...
Crystallization of the C-terminal domain of the bacteriophage T5 L-shaped fibre [0.03%]
噬菌体T5 L形纤丝C端结构域的结晶
Carmela Garcia-Doval,Daniel Luque,José R Castón et al.
Carmela Garcia-Doval et al.
Tails of bacteriophage T5 (a member of the Siphoviridae family) were studied by electron microscopy. For the distal parts of the L-shaped tail fibres, which are involved in host cell receptor binding, a low-resolution volume was calculated....
Crystallization and preliminary X-ray diffraction analysis of an active-site mutant of `loopless' family GH19 chitinase from Bryum coronatum in a complex with chitotetraose [0.03%]
来自Bryum coronatum的“无环”家族GH19几丁质酶活性位点突变体与 chitotetraose 复合物的结晶和初步X射线衍射分析
Takayuki Ohnuma,Naoyuki Umemoto,Toki Taira et al.
Takayuki Ohnuma et al.
The catalytic mechanism of family GH19 chitinases is not well understood owing to insufficient information regarding the three-dimensional structures of enzyme-substrate complexes. Here, the crystallization and preliminary X-ray diffraction...