Neutron fibre diffraction studies of amyloid using H2O/D2O isotopic replacement [0.03%]
利用H2O/D2O同位素置换法对淀粉样蛋白的中子纤维衍射研究
Sarah M Tiggelaar,Estelle Mossou,Phil Callow et al.
Sarah M Tiggelaar et al.
The first neutron fibre diffraction studies of an amyloid system are presented. The techniques used to prepare the large samples needed are described, as well as the procedures used to isotopically replace H2O in the sample by D2O. The resu...
Inducible production of recombinant human Flt3 ectodomain variants in mammalian cells and preliminary crystallographic analysis of Flt3 ligand-receptor complexes [0.03%]
哺乳动物细胞中诱导产生重组人FLT3胞外区变异体及FLT3配体-受体复合物的初步晶体学分析
Kenneth Verstraete,Bert Remmerie,Jonathan Elegheert et al.
Kenneth Verstraete et al.
The extracellular complex between the haematopoietic receptor Flt3 and its cytokine ligand (FL) is the cornerstone of signalling cascades that are central to early haematopoiesis and the immune system. Here, efficient protocols for the prod...
Purification, crystallization and preliminary crystallographic analysis of the CBS-domain protein MJ1004 from Methanocaldococcus jannaschii [0.03%]
来自甲烷高温产酸菌的CBS结构域蛋白MJ1004的纯化、结晶及初步晶体学分析
Iker Oyenarte,María Lucas,Inmaculada Gómez García et al.
Iker Oyenarte et al.
The purification and preliminary crystallographic analysis of the archaeal CBS-domain protein MJ1004 from Methanocaldococcus jannaschii are described. The native protein was overexpressed, purified and crystallized in the monoclinic space g...
Expression, purification and preliminary X-ray crystallographic analysis of cyanobacterial biliverdin reductase [0.03%]
细菌biliverdin还原酶的表达、纯化及初步X射线晶体学分析
Aya Watanabe,Kunio Hirata,Yoshinori Hagiwara et al.
Aya Watanabe et al.
Biliverdin reductase (BVR) catalyzes the conversion of biliverdin IX α to bilirubin IX α with concomitant oxidation of an NADH or NADPH cofactor. This enzyme also binds DNA and enhances the transcription of specific genes. Recombinant cya...
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of an ASCH domain-containing protein from Zymomonas mobilis ZM4 [0.03%]
芽孢单胞菌ZM4中含ASCH结构域的蛋白质的克隆、表达、纯化、结晶及初步X射线衍射分析
Suk-Youl Park,Jeong-Hoh Park,Jeong-Sun Kim
Suk-Youl Park
The human activating signal cointegrator 1 (ASC-1) homology (ASCH) domain is frequently observed in many organisms, although its function has not yet been clearly defined. In Zymomonas mobilis ZM4, the ZMO0922 gene encodes a polypeptide tha...
Crystallization and preliminary X-ray analysis of the TetR-like efflux pump regulator SimR [0.03%]
SimR脱氢酶类似物外排泵调节因子的结晶和初步X射线分析
Tung B K Le,Clare E M Stevenson,Mark J Buttner et al.
Tung B K Le et al.
Crystals of SimR were grown by vapour diffusion. The protein crystallized with trigonal symmetry and X-ray data were recorded to a resolution of 2.3 Å from a single crystal at the synchrotron. SimR belongs to the TetR family of bacterial t...
Structure of the extended-spectrum β-lactamase TEM-72 inhibited by citrate [0.03%]
柠檬酸抑制的扩展谱β-内酰胺酶TEM-72的结构
Jean Denis Docquier,Manuela Benvenuti,Vito Calderone et al.
Jean Denis Docquier et al.
TEM-72, a class A β-lactamase identified in isolates of Enterobacteriaceae, is a quadruple mutant of TEM-1 (Q39K, M182T, G238S and E240K) and shows extended-spectrum β-lactamase (ESBL) properties arising from the G238S and E240K substitut...
Pentapeptide-repeat proteins that act as topoisomerase poison resistance factors have a common dimer interface [0.03%]
五肽重复蛋白通过共用二聚体界面发挥作用以抵抗拓扑异构酶毒性的机制
Matthew W Vetting,Subray S Hegde,Yong Zhang et al.
Matthew W Vetting et al.
The protein AlbG is a self-resistance factor against albicidin, a nonribosomally encoded hybrid polyketide-peptide with antibiotic and phytotoxic properties produced by Xanthomonas albilineans. Primary-sequence analysis indicates that AlbG ...
Preparation, crystallization and preliminary X-ray diffraction analysis of two intestinal fatty-acid binding proteins in the presence of 11-(dansylamino)undecanoic acid [0.03%]
十二指肠脂肪酸结合蛋白的存在下 preparation，结晶和初步的x射线衍射分析
Aisha Laguerre,Jerome Wielens,Michael W Parker et al.
Aisha Laguerre et al.
Fatty-acid binding proteins (FABPs) are abundantly expressed proteins that bind a range of lipophilic molecules. They have been implicated in the import and intracellular distribution of their ligands and have been linked with metabolic and...
Expression, crystallization and preliminary X-ray analysis of the phosphoribosylglycinamide formyltransferase from Streptococcus mutans [0.03%]
变异链球菌磷核糖基甘氨酰胺甲酰转移酶的表达、结晶及初步X射线分析
Fangli Zhai,Xiaojuan Liu,Jing Ruan et al.
Fangli Zhai et al.
Phosphoribosylglycinamide formyltransferase (PurN) from Streptococcus mutans was recombinantly expressed in Escherichia coli. An effective purification protocol was established. The purified protein, which had a purity of >95%, was identifi...