NOD1 cooperates with HAX-1 to promote cell migration in a RIPK2- and NF-ĸB-independent manner [0.03%]
NOD1与HAX-1合作以一种RIPK2和NF-ĸB非依赖性的方式促进细胞迁移
Lucy Hezinger,Sarah Bauer,Kornelia Ellwanger et al.
Lucy Hezinger et al.
The human Nod-like receptor protein NOD1 is a well-described pattern-recognition receptor (PRR) with diverse functions. NOD1 associates with F-actin and its protein levels are upregulated in metastatic cancer cells. A hallmark of cancer cel...
Introduction of reversible cysteine ligation ability to the biliverdin-binding cyanobacteriochrome photoreceptor [0.03%]
具有结合胆绿素的蓝藻光受体引入可逆半胱氨酸连接能力
Takahisa Suzuki,Masataka Yoshimura,Hiroki Hoshino et al.
Takahisa Suzuki et al.
Cyanobacteriochrome (CBCR) photoreceptors are distantly related to the canonical red/far-red reversible phytochrome photoreceptors. In the case of the CBCRs, only the GAF domain is required for chromophore incorporation and photoconversion....
Applications of fluorescent protein tagging in structural studies of membrane proteins [0.03%]
荧光蛋白标记在膜蛋白结构研究中的应用
Ali A Kermani
Ali A Kermani
Generating active, pure, and monodisperse protein remains a major bottleneck for structural studies using X-ray crystallography and cryo-electron microscopy (cryo-EM). The current methodology heavily relies on overexpressing the recombinant...
Jürgen Scheller,Julia Ettich,Christoph Wittich et al.
Jürgen Scheller et al.
Interleukin-6 (IL-6)-type cytokines not only have key immunomodulatory functions that affect the pathogenesis of diseases such as autoimmune diseases, chronic inflammatory conditions, and cancer, but also fulfill important homeostatic tasks...
The role of sympathetic innervation in neonatal muscle growth and neuromuscular contractures [0.03%]
新生儿肌肉生长和神经肌肉挛缩中交感神经支配的作用
Mason T Runkel,Albaraa Tarabishi,Kritton Shay-Winkler et al.
Mason T Runkel et al.
Neonatal brachial plexus injury (NBPI), a leading cause of pediatric upper limb paralysis, results in disabling and incurable muscle contractures that are driven by impaired longitudinal growth of denervated muscles. A rare form of NBPI, wh...
PAR recognition by PARP1 regulates DNA-dependent activities and independently stimulates catalytic activity of PARP1 [0.03%]
PAR识别结合可调节PARP1的DNA依赖性功能并独立刺激PARP1催化活性
Waghela Deeksha,Suman Abhishek,Eerappa Rajakumara
Waghela Deeksha
Poly(ADP-ribosyl)ation is predominantly catalyzed by Poly(ADP-ribose) polymerase 1 (PARP1) in response to DNA damage, mediating the DNA repair process to maintain genomic integrity. Single-strand (SSB) and double-strand (DSB) DNA breaks are...
Regio- and stereoselective steroid hydroxylation by CYP109A2 from Bacillus megaterium explored by X-ray crystallography and computational modeling [0.03%]
X射线晶体学和计算建模探索了巨大芽孢杆菌CYP109A2的选择性皮质醇羟基化机制
Ilona K Jóźwik,Elvira Bombino,Ammar Abdulmughni et al.
Ilona K Jóźwik et al.
The P450 monooxygenase CYP109A2 from Bacillus megaterium DSM319 was previously found to convert vitamin D3 (VD3) to 25-hydroxyvitamin D3. Here, we show that this enzyme is also able to convert testosterone in a highly regio- and stereoselec...
Dimerization choice and alternative functions of ZBTB transcription factors [0.03%]
ZBTB转录因子的二聚化选择和功能多样性
Sarah Barakat,Ege Ezen,İzem Devecioğlu et al.
Sarah Barakat et al.
Zinc Finger DNA-binding domain-containing proteins are the most populous family among eukaryotic transcription factors. Among these, members of the BTB domain-containing ZBTB sub-family are mostly known for their transcriptional repressive ...
Structure-function analysis of bacterial GH31 α-galactosidases specific for α-(1→4)-galactobiose [0.03%]
细菌GH31 α-半乳糖苷酶的结构功能分析该酶特异于α(1->4)半乳双糖
Marina Ikegaya,Enoch Y Park,Takatsugu Miyazaki
Marina Ikegaya
Glycoside hydrolase family 31 (GH31) contains α-glycoside hydrolases with different substrate specificities involved in various physiological functions. This family has recently been classified into 20 subfamilies using sequence similarity...
Structural insights into hydrolytic defluorination of difluoroacetate by microbial fluoroacetate dehalogenases [0.03%]
微生物氟乙酸脱卤酶催化的difluoroacetate水解氟化作用的结构洞察
Anna N Khusnutdinova,Khorcheska A Batyrova,Greg Brown et al.
Anna N Khusnutdinova et al.
Fluorine forms the strongest single bond to carbon with the highest bond dissociation energy among natural products. However, fluoroacetate dehalogenases (FADs) have been shown to hydrolyze this bond in fluoroacetate under mild reaction con...