An inverse small molecule screen to design a chemically defined medium supporting long-term growth of Drosophila cell lines [0.03%]
一种逆向小分子筛选以设计支持果蝇细胞系长期生长的化学界定培养基
M Burnette,T Brito-Robinson,J Li et al.
M Burnette et al.
Drosophila cell culture is used as a model system with multiple applications including the identification of new therapeutic targets in screens, the study of conserved signal transduction pathway mechanisms, and as an expression system for ...
Effects of pseudophosphorylation mutants on the structural dynamics of smooth muscle myosin regulatory light chain [0.03%]
伪磷酸化变异对平滑肌肌球蛋白调节轻链结构动态的影响
L Michel Espinoza-Fonseca,Brett A Colson,David D Thomas
L Michel Espinoza-Fonseca
We have performed 50 independent molecular dynamics (MD) simulations to determine the effect of pseudophosphorylation mutants on the structural dynamics of smooth muscle myosin (SMM) regulatory light chain (RLC). We previously showed that t...
HIF-1 promotes the expression of its α-subunit via an epigenetically regulated transactivation loop [0.03%]
HIF-1通过表观遗传调控的转激活环促进其α亚基的表达
D J Asby,F Cuda,F Hoakwie et al.
D J Asby et al.
Hypoxia inducible factor-1 (HIF-1) directs the cellular response to low oxygen and plays a key role in tumour survival and growth. Here we use an inhibitor of the HIF-1α/HIF-1β protein-protein interaction to show the presence of an epigen...
Sighting of tankyrase inhibitors by structure- and ligand-based screening and in vitro approach [0.03%]
基于结构和配体的筛选及体外实验发现tankyrase抑制剂
Palani Kirubakaran,Pitchaimani Arunkumar,Kumpati Premkumar et al.
Palani Kirubakaran et al.
Tankyrase 1 and 2 (TNKS) are promising and attractive therapeutic targets in anticancer drug development. Herein, we report the findings of structure- and ligand-based virtual screening for novel TNKS1 inhibitors using iterative rounds of i...
Encompassing receptor flexibility in virtual screening using ensemble docking-based hybrid QSAR: discovery of novel phytochemicals for BACE1 inhibition [0.03%]
基于系综对接的杂合QSAR在虚拟筛选中的受体灵活性包容：发现新的植物化学成分抑制BACE1
Sandipan Chakraborty,Balaji Ramachandran,Soumalee Basu
Sandipan Chakraborty
Mimicking receptor flexibility during receptor-ligand binding is a challenging task in computational drug design since it is associated with a large increase in the conformational search space. In the present study, we have devised an in si...
Plasma membrane translocation of a protein needle based on a triple-stranded β-helix motif [0.03%]
基于三链β-螺旋基序的蛋白质针在质膜上的转移
Nusrat J M Sanghamitra,Hiroshi Inaba,Fumio Arisaka et al.
Nusrat J M Sanghamitra et al.
Plasma membrane translocation is challenging due to the barrier of the cell membrane. Contrary to the synthetic cell-penetrating materials, tailed bacteriophages use cell-puncturing protein needles to puncture the cell membranes as an initi...
Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis [0.03%]
野油菜黄单胞菌葡萄糖利用代谢通量模式：恩特纳-道多罗夫途径为主，磷酸戊糖途径和糖酵解为辅
Sarah Schatschneider,Claudia Huber,Heiko Neuweger et al.
Sarah Schatschneider et al.
The well-studied plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) synthesizes the biotechnologically important polysaccharide xanthan gum, which is also regarded as a virulence factor in plant interactions. In Xcc, sug...
A metabonomic analysis of the effect of quercetin on toxicity induced by chronic exposure to low-level dichlorvos in rat plasma [0.03%]
槲皮素对慢性低剂量敌敌畏暴露致大鼠毒性作用的代谢组学分析研究
Yan Zeng,Lei Qi,Sifan Li et al.
Yan Zeng et al.
A previous study of ours has reported that chronic exposure to low-level dichlorvos (DDVP, 7.2 mg per kg bw) damages the liver, interferes with fatty acid metabolism, and disturbs the antioxidant defense system in rats. This study aims to i...
Informative Bayesian Model Selection: a method for identifying interactions in genome-wide data [0.03%]
一种从全基因组数据中识别相互作用的方法：有启发性的贝叶斯模型选择方法
Mehran Aflakparast,Ali Masoudi-Nejad,Joseph H Bozorgmehr et al.
Mehran Aflakparast et al.
In high-dimensional genome-wide (GWA) data, a key challenge is to detect genomic variants that interact in a nonlinear fashion in their association with disease. Identifying such genomic interactions is important for elucidating the inherit...
Proteome-wide analysis of human disease mutations in short linear motifs: neglected players in cancer? [0.03%]
人类疾病突变在癌症中的疏水区域分析
Bora Uyar,Robert J Weatheritt,Holger Dinkel et al.
Bora Uyar et al.
Disease mutations are traditionally thought to impair protein functionality by disrupting the folded globular structure of proteins. However, 22% of human disease mutations occur in natively unstructured segments of proteins known as intrin...