Brian Cieslewicz,Cullen Roeder,Dara Bree et al.
Brian Cieslewicz et al.
Several technologies leverage avidin/biotin interactions for complexation including the MAPS vaccine technology which utilizes rhizavidin, a biotin-binding protein derived from the proteobacterium Rhizobium etli, to complex antigens with bi...
VIF-Ig: A novel Fc framework for ADCC by incorporation of VHH unit [0.03%]
一种新型的ADCC效应Fc框架（VIF-Ig）：通过嵌合纳米抗体可变区片段实现
Yaohui Li,Yichen Huang,Yingdan Duan et al.
Yaohui Li et al.
Antibody-dependent cellular cytotoxicity is a key mechanism for antibody-based therapeutics, and current engineering strategies to enhance ADCC primarily rely on two approaches: Fc mutations to improve the antibody's intrinsic CD16a affinit...
Rapid Isolation and Characterization of High-Affinity Single-Domain Antibodies for Broad-Spectrum Detection of SARS-CoV-2 Variants [0.03%]
快速分离和表征高亲和力单域抗体以检测SARS-CoV-2变异毒株
Ah Hyun Cho,Hee Eon Lee,Ji Hyun Lee et al.
Ah Hyun Cho et al.
The rapid evolution of SARS-CoV-2 presents substantial challenges to maintaining diagnostic accuracy. Single-domain antibodies (variable domains of camelid heavy-chain antibodies, VHHs), commonly referred to as nanobodies, are attractive to...
A His-to-Gln mutation in catechol 2,3-dioxygenase catalyzes 2,3-dihydroxybenzoic acid cleavage by an intradiol pathway [0.03%]
catechol 2,3-二氧酶中His变为Gln的突变使其催化2,3-二羟基苯甲酸的裂解反应转变为间径途径类型反应
Deepti Singh,Gurunath Ramanathan
Deepti Singh
Catechol 2,3-dioxygenases (C23DO) catalyze extradiol cleavage of catechol in aromatic hydrocarbon degradation pathways. Here, we report mutagenesis studies on two C23DO isoenzymes from Diaphorobacter sp. strain DS2, aimed at elucidating the...
Marc Zimmer
Marc Zimmer
Model organisms significantly advance research because scientific communities recognize their value, develop infrastructure around them, and utilize them to address questions relevant to multiple species. I propose adopting a similar approa...
Rational design of selective bispecific EPO-R/CD131 agonists [0.03%]
理性设计选择性双特异性促红细胞生成素受体(EPO-R)/CD131激动剂
Kailyn E Doiron,Jeffrey C Way,Pamela A Silver
Kailyn E Doiron
Erythropoietin (EPO) suppresses apoptosis and promotes survival by signaling through EPO-R/EPO-R on hematopoietic progenitors or EPO-R/CD131 on non-hematopoietic cells. However, EPO signaling through EPO-R/CD131 is controversial and there i...
Design of a ligand-dependent fluorescent biosensor, based on an engineered lipocalin (Anticalin), for the sensitive detection of the Alzheimer β-amyloid peptide [0.03%]
基于工程化脂质运载蛋白(Anticalin)的依赖配体荧光生物传感器的设计，用于阿尔茨海默病β淀粉样蛋白肽的灵敏检测
Anna Feuerbach,Arne Skerra
Anna Feuerbach
Based on the Anticalin H1GA which tightly binds Aβ40 and Aβ42 peptides - both established biomarkers of Alzheimer's disease - we describe the design of a protein-dye conjugate as analytical reagent that shows strongly elevated fluorescenc...
Enzyme-substrate co-display on yeast empowers engineering of tobacco etch virus protease activity [0.03%]
基于酵母的酶-底物共展示技术增强烟草蚀纹病毒蛋白酶活性工程改造
Brian M Mikolajczyk,Alexander W Golinski,Benjamin J Hackel
Brian M Mikolajczyk
Engineering improved protease activity using directed evolution is challenged by uncertainty in sequence-function mapping and inefficiency in evaluating activity of candidate mutants. We implemented a generalizable yeast surface display app...
Alba Pejenaute,Laura Gálvez-Larrosa,Cristina M Segovia et al.
Alba Pejenaute et al.
Nanobodies offer unique advantages in biomedical and biotechnological applications due to their smaller size, ability to bind challenging epitopes, and affordable production using recombinant technology. However, challenges in large-scale p...
Linker minimization and characterization of fc-fused interleukin-17A for increased in vivo half-life [0.03%]
一种Fc融合型白细胞介素-17A的连接臂优化及体内半衰期延长研究
Qiu C Wu,Jungmin Lee,Aishwarya Swaminathan et al.
Qiu C Wu et al.
Interleukin-17A (IL-17A) is a cytokine involved in pro-inflammatory responses and tissue regeneration, with potential therapeutic and research applications. However, its short serum half-life limits in vivo use. Here, we report the systemat...