Incorporation of azide groups into an engineered Fn3 scaffold protein for site-specific, bioorthogonal conjugation [0.03%]
氮丙基官能团化的Fn3蛋白支架用于生物正交点击反应
Kristine T Le,Tatum R McKenna,Pauline M Mallari et al.
Kristine T Le et al.
Protein bioconjugates have a wide range of applications, including for targeted drug delivery and in vivo diagnostics. Expanding the chemistry available to link protein to cargo remains a critical consideration for the development of novel ...
Assessing Structural Prediction Accuracy for Nanobody-Small Molecule Complexes [0.03%]
纳米抗体-小分子复合物的结构预测准确性评估
Berta Bori-Bru,Juan-Pablo Salvador,Ramon Crehuet
Berta Bori-Bru
Generative models have transformed structural bioinformatics, enabling antibody and nanobody design against protein epitopes; however, nanobody engineering for small molecule sensing remains largely experimental. In this work, we evaluate s...
Hui-Wang Ai,Frances H Arnold,Doug Barrick et al.
Hui-Wang Ai et al.
With this status report, we aim to provide a timely snapshot of the protein engineering field as a broad and rapidly advancing discipline that integrates computational, molecular biology, structure-guided, evolutionary, and synthetic approa...
A Tethered Yeast Surface Display System Facilitates Enriching Epitope-Specific Binding Interactions [0.03%]
一种束缚的酵母菌表面展示系统利于纯化特异位点的结合反应
Rojhae A Panton,Jakob Lind,Amanda Richardson et al.
Rojhae A Panton et al.
Engineering proteins for therapeutic applications is a field that has seen substantial growth in the past two decades, but challenges remain. A major deficiency in current strategies is the lack of a means to efficiently screen for binding ...
Free energy-guided Q302P mutation in GH1 β-glucosidase from Aspergillus fumigatus improves enzyme function via catalytic tunnel perturbation [0.03%]
自由能引导的吉卜赛人雾状芽枝霉菌GH1 β-葡萄糖苷酶Q302P突变通过催化隧道扰动改善酶功能
Shalini Das,Subba Reddy Dodda,Musaddique Hossain et al.
Shalini Das et al.
Beta-glucosidase (EC 3.2.1.21) is crucial to biorefinery industries because it is the bottleneck enzyme in the enzymatic conversion of cellobiose to glucose, a key component of the enzyme complex that converts lignocellulosic biomass into g...
Engineering of tandem bispecific IL-7 receptor agonist antibody promoting selective T cell expansion [0.03%]
工程化的串联双特异性白细胞介素-7受体激动剂抗体促进T细胞的选择性增殖
Jun-Kook Park,Inseong Jung,Seunghyun Lee et al.
Jun-Kook Park et al.
Interleukin-7 (IL-7) is indispensable for T cell development and homeostasis, and clinical studies have demonstrated its ability to restore T cell numbers in lymphopenic conditions. However, the therapeutic application of IL-7 has been limi...
Prioritizing Stability-enhancing Mutations using the ESM Protein Language Model in conjunction with Physics-based MM/GBSA Predictions [0.03%]
基于物理的分子模拟和语言模型联合预测蛋白质稳定性增强型突变优先级研究
Emily R Rhodes,Guido Scarabelli,Jonathan Jou et al.
Emily R Rhodes et al.
Directed evolution for protein engineering, as currently practiced in the biotechnology and pharmaceutical industries, is both tedious and expensive. Computationally driven protein design has the potential to expedite the engineering proces...
Mapping functional dynamics hotspots for protein engineering with NMR peak intensity analysis [0.03%]
基于核磁峰强度分析指导蛋白质工程中功能动态热点的绘制
Adam M Damry,Serena E Hunt,Sandrine Legault et al.
Adam M Damry et al.
Structural dynamics play a crucial role in protein function, and tuning these dynamics through mutagenesis has emerged as a promising strategy for enhancing activity. However, identifying dynamics hotspots for protein engineering remains a ...
Combining bacterial display and protein language models to engineer a CD69-binding affibody for molecular imaging of immune activation [0.03%]
结合细菌展示技术和蛋白质语言模型工程化CD69结合Affibody分子以实现免疫激活的分子成像
Hugo Olsson,Cornelia Westerberg,Jonas Persson et al.
Hugo Olsson et al.
Recent years have seen remarkable clinical success with therapies that harness the patient's immune system, with checkpoint inhibitors in oncology as a prominent example. Non-invasive monitoring of immune activation in vivo has the potentia...