Response Surface Methodology to Optimize the Expression Efficiency of Recombinant Reteplase [0.03%]
响应面法优化重组替降钙素的表达效应
Farhad Farzaneh,Sako Mirzaie,Ehsan Dehnavi et al.
Farhad Farzaneh et al.
Background: Over expression of Reteplase enzyme has already been studies in the periplasmic space of Escherichia coli (E. coli). However, the role different factors in its expresssin rate remained to be elucidated. ...
Efficient CRISPR/Cas9-Mediated BAX Gene Ablation in CHO Cells To Impair Apoptosis and Enhance Recombinant Protein Production [0.03%]
高效CRISPR / Cas9介导的CHO细胞中BAX基因消融以阻碍凋亡和增强重组蛋白生产
Amirabbas Rahimi,Morteza Karimipoor,Reza Mahdian et al.
Amirabbas Rahimi et al.
Background: Despite recent advances in recombinant biotherapeutics production using CHO cells, their productivity remains lower than industrial needs, mainly due to apoptosis. ...
Pan-cancer Analysis Reveals SRC May Link Lipid Metabolism and Macrophages [0.03%]
泛癌分析揭示SRC可能连接脂质代谢和巨噬细胞
Zhongyuan Chen,Yaqian Xiao,Pinhong Yang et al.
Zhongyuan Chen et al.
Background: SRC is a member of the membrane-associated non-receptor protein tyrosine kinase superfamily. It has been reported to mediate inflammation and cancer. However, the exact molecular mechanism involved is still no...
Multi-Objective Optimization of Copper Bioleaching: Comparative Study of Pure and Co-Cultured Cultivation [0.03%]
铜浸出的多目标优化：纯培养及共培养的比较研究
Yasin Rakhshani,Sayyed Shahryar Rahpeyma,Fatemeh Tabandeh et al.
Yasin Rakhshani et al.
Background: Bioleaching is a practical method to recover metals from low-grade mineral sulfides. The most frequent bacteria involved in the bioleaching of metals from ores are Acidithiobacillus ferrooxidans and Acidithiob...
Preparation and Characterization of Crayfish (Astacus leptodactylus) Chitosan with Different Deacetylation Degrees [0.03%]
不同脱乙酰度的欧洲螯虾（Astacus leptodactylus）壳聚糖的制备及表征
Ali Eslem Kadak,Aygül Küçükgülmez,Mehmet Çelik
Ali Eslem Kadak
Background: In this study, chitosan with various deacetylation degrees was extracted from crayfish (Astacus leptodactylus) shells with the purpose of examining the effect of deacetylation on the characterization of chitos...
Evaluation of Apoptosis, Cell Proliferation and Cell Cycle Progression by Inactivation of the NEAT1 Long Noncoding RNA in a Renal Carcinoma Cell Line Using CRISPR/Cas9 [0.03%]
利用CRISPR/Cas9失活NEAT1长链非编码RNA对肾癌细胞系凋亡、增殖和细胞周期进程的评估
Nastaran Haghighi,Abbas Doosti,Jafar Kiani
Nastaran Haghighi
Background: Long noncoding RNAs (lncRNAs) play an important role in cellular mechanisms including transcription, translation, and apoptosis. NEAT1 is one of the essential types of lncRNAs in humans that can bind to active...
TNF-α Regulates the Glucocorticoid Receptor Alpha Expression in Human Nasal Epithelial Cells Via p65-NF-κb and p38-MAPK Signaling Pathways [0.03%]
肿瘤坏死因子α通过p65NFкB和p38MAPK信号通路调节人鼻粘膜上皮细胞糖皮质激素受体Alpha的表达
Yongquan Jiang,Bin Liu,Ximing Bao et al.
Yongquan Jiang et al.
Background: Tumor necrosis factor (TNF)-α induces changes in the glucocorticoid receptor (GR) isoforms' expression in human nasal epithelial cells (HNECs) in chronic rhinosinusitis (CRS). ...
Phytate-Induced Dose-Response Auto-Activation of Enzyme in Commercial Recombinant Phytase From Escherichia coli [0.03%]
植酸诱导的商业重组植酸酶自动激活的剂量效应关系研究
Elmira Naghdi,Zahra Moosavi-Nejad,Bahman Gholamhossein Goudarzi et al.
Elmira Naghdi et al.
Background: Microbial phytase is one of the most widely used enzymes in food industries like cattle, poultry, and aquaculture food. Therefore, understanding the kinetic properties of the enzyme is very important to evalua...
MiRNA-106a-5p Promotes Laryngeal Carcinoma Proliferation and Migration Through PI3K/AKT/m-TOR Pathway by AKTIP [0.03%]
microRNA-106a-5p通过AKTIP促进PI3K/AKT/m-TOR途径及喉癌增殖和迁移
Liang Gong,Xue-Feng Wang,Hao Liu et al.
Liang Gong et al.
Background: Laryngeal cancer (LC) remains one of the most common tumors of the respiratory tract, the exact pathogenesis remains unclear. MiRNA-106a-5p is aberrantly expressed in a variety of cancers and plays a pro- or a...
Insights Into The Effects of Amino Acid Substitutions on The Stability of Reteplase Structure: A Molecular Dynamics Simulation Study [0.03%]
基于分子动力学模拟的氨基酸取代对雷替普酶结构稳定性影响研究的新见解
Kaveh Haji-Allahverdipoor,Mokhtar Jalali Javaran,Sajad Rashidi Monfared et al.
Kaveh Haji-Allahverdipoor et al.
Background: Reteplase (recombinant plasminogen activator, r-PA) is a recombinant protein designed to imitate the endogenous tissue plasminogen activator and catalyze the plasmin production. It is known that the applicatio...