The haematopoietic stem cell niche: new insights into the mechanisms regulating haematopoietic stem cell behaviour [0.03%]
造血干细胞生态位：调控造血干细胞行为的新机制初探
Andrew J Lilly,William E Johnson,Christopher M Bunce
Andrew J Lilly
The concept of the haematopoietic stem cell (HSC) niche was formulated by Schofield in the 1970s, as a region within the bone marrow containing functional cell types that can maintain HSC potency throughout life. Since then, ongoing researc...
Generation of Induced Pluripotent Stem (iPS) Cells by Nuclear Reprogramming [0.03%]
由细胞核重新编程诱导产生多能干细胞(iPS细胞)
Dilip Dey,Gregory R D Evans
Dilip Dey
During embryonic development pluripotency is progressively lost irreversibly by cell division, differentiation, migration and organ formation. Terminally differentiated cells do not generate other kinds of cells. Pluripotent stem cells are ...
Erythroblast enucleation [0.03%]
红细胞前体脱核现象
Ganesan Keerthivasan,Amittha Wickrema,John D Crispino
Ganesan Keerthivasan
Even though the production of orthochromatic erythroblasts can be scaled up to fulfill clinical requirements, enucleation remains one of the critical rate-limiting steps in the production of transfusable red blood cells. Mammalian erythrocy...
Hepatic differentiation of murine disease-specific induced pluripotent stem cells allows disease modelling in vitro [0.03%]
特异性疾病的诱导多能干细胞的肝细胞分化允许建立体外疾病模型
Reto Eggenschwiler,Komal Loya,Malte Sgodda et al.
Reto Eggenschwiler et al.
Direct reprogramming of somatic cells into pluripotent cells by retrovirus-mediated expression of OCT4, SOX2, KLF4, and C-MYC is a promising approach to derive disease-specific induced pluripotent stem cells (iPSCs). In this study, we focus...
Efficient non-viral integration and stable gene expression in multipotent adult progenitor cells [0.03%]
高效的非病毒整合及多能成人前体细胞的稳定基因表达
Andrew Wilber,Fernando Ulloa Montoya,Luke Hammer et al.
Andrew Wilber et al.
Non-viral integrating systems, PhiC31 phage integrase (ϕC31), and Sleeping Beauty transposase (SB), provide an effective method for ex vivo gene delivery into cells. Here, we used a plasmid-encoding GFP and neomycin phosphotransferase alon...
Toru Egashira,Shinsuke Yuasa,Keiichi Fukuda
Toru Egashira
Induced pluripotent stem (iPS) cells are generated by reprogramming human somatic cells through the forced expression of several embryonic stem (ES) cell-specific transcription factors. The potential of iPS cells is having a significant imp...
Nuclear reprogramming in mouse primordial germ cells: epigenetic contribution [0.03%]
小鼠原始生殖细胞中的核重新编程：表观遗传贡献
Massimo De Felici
Massimo De Felici
The unique capability of germ cells to give rise to a new organism, allowing the transmission of primary genetic information from generation to generation, depends on their epigenetic reprogramming ability and underlying genomic totipotency...
Phenotypic definition of the progenitor cells with erythroid differentiation potential present in human adult blood [0.03%]
人成人血液中具有红细胞分化潜能的祖细胞的表型定义
Valentina Tirelli,Barbara Ghinassi,Anna Rita Migliaccio et al.
Valentina Tirelli et al.
In Human Erythroid Massive Amplification (HEMA) cultures, AB mononuclear cells (MNC) generate 1-log more erythroid cells (EBs) than the corresponding CD34(pos) cells, suggesting that MNC may also contain CD34(neg) HPC. To clarify the phenot...
Guillaume Pourcher,Christelle Mazurier,Yé Yong King et al.
Guillaume Pourcher et al.
We previously described the large-scale production of RBCs from hematopoietic stem cells (HSCs) of diverse sources. Our present efforts are focused to produce RBCs thanks to an unlimited source of stem cells. Human embryonic stem (ES) cells...
An inducible expression system of the calcium-activated potassium channel 4 to study the differential impact on embryonic stem cells [0.03%]
一种可诱导表达钙激活钾通道4的体系以研究其对胚胎干细胞的不同影响
Stefan Liebau,Michael Tischendorf,Daniel Ansorge et al.
Stefan Liebau et al.
Rationale. The family of calcium-activated potassium channels consists of four members with varying biological functions and conductances. Besides membrane potential modulation, SK channels have been found to be involved in cardiac pacemake...