Controlled CRISPR/Cas12a Activation via DNAzyme-Mediated Splitting of Chimeric Substrate for Lead Detection [0.03%]
一种DNA酶介导的嵌合底物分裂控制CRISPR / Cas12a激活体系用于铅离子检测
Peiying Zhang,Meng Shen,Lihua Ding et al.
Peiying Zhang et al.
Chronic lead exposure poses severe threats to human health, which demands a rapid detection strategy beyond conventional instrumentation-dependent approaches. While CRISPR/Cas12a systems offer promising alternatives through trans-cleavage a...
Global Lysine 2-Hydroxyisobutyrylation Profiling Reveals the Growth Mechanism of Lactobacillus paracasei PC-01 in High-density Cultures [0.03%]
全局L-lysine 2-羟异丁酰化修饰分析揭示了植物乳杆菌PC-01高密度培养的生长机制
Shijia Shen,Runzhi Zhou,Hua Wang et al.
Shijia Shen et al.
The importance of lysine 2-hydroxyisobutyrylation (Khib) in regulating biological processes has become increasingly apparent, but its functional significance in prokaryotes, especially in the Lactobacillus paracasei PC-01, is poorly underst...
Overview of Bacterial Cellulose: Biosynthesis Strategies, Functionalization and Biomedical Marketing [0.03%]
细菌纤维素概览：生物合成策略、功能化及生物医药市场展望
Ahmed K Saleh,Tarek H Taha,Hussain Alenezi et al.
Ahmed K Saleh et al.
Bacterial cellulose (BC) is reported as an extracellular polysaccharide distinguished by its exceptional purity, mechanical features, and biocompatibility, making it an attractive material for applications in food, plant tissue culture, and...
High oral doses of vitamin B12 in early pregnancy improve B12 status of mother and child irrespective of subsequent supplementation A longitudinal study in B12 insufficient Indian women supplemented with either milk, vitamin pills, or placebo [0.03%]
叶酸早期适量口服高剂量维生素B12可改善母体及后代维生素B12的状态——一次关于对印度缺乏维生素B12的女性进行牛奶、维生素片或安慰剂补充的纵向研究
Sadanand Naik,Sergey N Fedosov,Namita Mahalle et al.
Sadanand Naik et al.
B12 deficiency during pregnancy has been associated with shunted growth, neural tube defects, anemia, and neuro-cognitive disorders. The optimal prevention strategy remains to be found. Here we explore the effect of high doses of oral B12 i...
The regulation characterization of novel transcription regulator JTY_2262 in Mycobacterium bovis BCG [0.03%]
结核分枝杆菌卡介苗株新型转录调节因子JTY_2262的表征研究
Jinmiao Song,Hui Wang,Mingrui Sun et al.
Jinmiao Song et al.
Mycobacterium tuberculosis (Mtb) can survive for a long time in vivo and evade host immune attacks, primarily through transcriptional regulation mediated by transcription regulators. JTY_2262 (homologous with Rv2250c) belongs to the Tetracy...
Micronutrients Shape FOXP2 Activity: Mechanistic Insights from Retinoic Acid, Folic Acid and Pyrroloquinoline Quinone [0.03%]
微量营养素调节FOXP2活性：由视黄酸、叶酸和吡咯喹啉醌研究所得的机制见解
Mande Goldfein,Sylvia Fanucchi
Mande Goldfein
FOXP2 is a neurodevelopmental transcription factor that regulates genes essential for neuronal differentiation, synaptic connectivity, and speech and language. Here, we show that the small neuroactive molecules retinoic acid (RA), folic aci...
Materials and Device Architectures for Cortisol Detection: Mechanisms, Measurement Technologies, and Clinical Implications [0.03%]
用于皮质醇检测的材料和器件架构：机制、测量技术和临床意义
Monali Garg,Syed Gulfishan,Bi Bi Zainab Mazhari et al.
Monali Garg et al.
Cortisol, the primary glucocorticoid produced in response to stress, can be a chronic health risk or a protective partner. Cortisol spikes can help people survive by triggering energy mobilization, immune regulation, and neuroendocrine bala...
Assessing the role of E451, D452, and H139 in the catalytic function of a Thermus thermophilus bacterial laccase [0.03%]
热菌乳酸菌漆酶中E451、D452和H139在催化功能中的作用评估
R Miranda-Blancas,M C Cardona-Echavarría,G Saab-Rincón et al.
R Miranda-Blancas et al.
Laccases are oxidoreductase enzymes that require four electrons and four protons for each catalytic cycle to trigger the conversion of one oxygen molecule into two water molecules. However, for bacterial laccases, including the laccase from...
ShortCut RNase III is capable of digesting single mRNA transcripts into < 10 nucleotide products [0.03%]
短链RNase III能够将单一信使核糖核酸剪切为少于10个核苷酸的产物
Kyle J Tynan,Mark S Lowenthal,Karndeep Singh et al.
Kyle J Tynan et al.
RNase III is a dsRNA specific ribonuclease that has been used to enzymatically digest dsRNA impurities generated during in vitro transcription. Here, we demonstrate that ShortCut RNase III, a recombinant version of E. coli RNase III fused w...
The shifting sands of venom: Divergent blood clotting factor activation pathways and differential Factor Va co-factor dependence for the venoms of Middle Eastern desert vipers (Eristicophis and Pseudocerastes species) [0.03%]
中东沙漠响尾蛇（Eristicophis和Pseudocerastes物种）毒液中不同的凝血因子激活途径及对因子Va辅因子的依赖性差异：流沙中的毒液
Patrick S Champagne,Lorenzo Seneci,Behzad Fathinia et al.
Patrick S Champagne et al.
Desert vipers (Eristicophis and Pseudocerastes) employ diverse procoagulant strategies despite their close phylogenetic relationships, yet the biochemical mechanisms underlying the variations in their coagulotoxic activities remain poorly u...