A two-signal aptasensor for sensitive exosome detection via rolling circle amplification and G-quadruplex structure [0.03%]
基于滚环放大和G-四链体结构的双信号适配子传感器用于敏感检测外泌体
Xu Sun,Qing Dong,Xuemei Zhang et al.
Xu Sun et al.
Exosomes play a pivotal role in cancer diagnosis and therapy, yet their clinical application faces significant technical challenges. To address this, we developed a sensitive, aptamer-based platform that integrates nucleic acid amplificatio...
Optimization of the angle for scattered light measurements in 3D-printed cultivation vessels [0.03%]
优化三维培养容器中散射光测量的角度
Nicolas Debener,Louis Maximilian Kuhnke,Sascha Beutel et al.
Nicolas Debener et al.
Monitoring key parameters during the cultivation of microorganisms-including biomass concentration-is often a crucial prerequisite for attaining reproducible results in the field of biotechnology. In order to overcome the drawbacks associat...
Optimization of the angle for scattered light measurements in 3D-printed cultivation vessels [0.03%]
基于3D打印培养容器的散射光测量角度优化
Nicolas Debener,Louis Maximilian Kuhnke,Sascha Beutel et al.
Nicolas Debener et al.
Monitoring key parameters during the cultivation of microorganisms-including biomass concentration-is often a crucial prerequisite for attaining reproducible results in the field of biotechnology. In order to overcome the drawbacks associat...
Sterol analysis in cancer cells using atmospheric pressure ionization techniques [0.03%]
基于大气压离子化技术的肿瘤细胞固醇分析研究
Pia Wittenhofer,Juan F Ayala-Cabrera,Laila Orell et al.
Pia Wittenhofer et al.
The analysis of sterol biosynthesis poses analytical challenges such as vast concentration differences between precursor molecules and cholesterol as well as the substantial interferences between the sterols and the matrix, resulting in str...
Next-generation protein sequencing and individual ion mass spectrometry enable complementary analysis of interleukin-6 [0.03%]
新一代蛋白质测序与单离子质量光谱互补分析白细胞介素-6
Kenneth A Skinner,Troy D Fisher,Andrew Lee et al.
Kenneth A Skinner et al.
The vast complexity of the proteome currently overwhelms any single analytical technology in capturing the full spectrum of proteoform diversity. In this study, we evaluated the complementarity of two cutting-edge proteomic technologies-sin...
Glycoproteomics analysis of colorectal cancer reveals differential alterations of glycoproteins and glycans [0.03%]
糖蛋白组学分析揭示结直肠癌中糖蛋白和糖链的差异性改变
Wenqi Zhang,Xiaotong Wang,Shengye Wen et al.
Wenqi Zhang et al.
Colorectal cancer (CRC) remains a global health concern, with an urgent need for better early detection and targeted therapies. Our glycoproteomics analysis comparing CRC to paracancerous (PCA) tissue revealed significant alterations. Globa...
Resolving agrochemical penetration in wheat leaves with secondary ion mass spectrometry imaging and depth profiling [0.03%]
利用二次离子质谱成像和深度剖面解析小麦叶片中农药的渗透性问题
Akhila Ajith,Sadia Sheraz,Aline Xavier de Souza et al.
Akhila Ajith et al.
The foliar application of an agrochemical is first encountered by the hydrophobic cuticle and then the hydrophilic epidermis of the leaf. Understanding the movement and distribution of pesticides through these layers is vital in the researc...
Tetracycline detection via carbon dots-Fe coordination from Chromolaena odorata using a portable device [0.03%]
用便携式设备基于扶芳藤碳点-铁配位检测四环素
Nguyen Huynh Dang Khoa,Nguyen Ngoc Nghia,Bui The Huy et al.
Nguyen Huynh Dang Khoa et al.
Tetracycline residues in aquatic ecosystems, resulting from widespread antibiotic use, pose significant risks by fostering antibiotic resistance and threatening ecological and human health. Addressing the need for rapid, sensitive, and port...
Demonstrating voxel-by-voxel (V × V) single-point calibration in liver tissue by IR-MALDESI quantitative MSI [0.03%]
基于IR-MALDESI定量质谱成像的肝组织体素对体素质子弛豫时间单点校正方法研究
Emily R Bruce,Russell R Kibbe,Logan J Opperman et al.
Emily R Bruce et al.
Quantitative mass spectrometry imaging (qMSI) provides information regarding the colocalization, relative abundance, and concentration of a target analyte in a tissue without homogenization. Ionization sources, including IR-MALDESI, commonl...
Anti-interference labeled SERS lateral flow immunoassay based on Prussian blue for ultra-sensitive quantitative detection of bloodstains in forensic field [0.03%]
一种基于普鲁士蓝的防干扰标签SERS侧流免疫分析法在法医现场用于血痕超灵敏定量检测
Long Chen,Feng Wang,Ziwen Guo et al.
Long Chen et al.
In the field investigation of criminal cases, the tracking of blood evidence is very important. However, post-crime bloodstains are often cleaned up or covered up, making residual traces difficult to detect. Consequently, the creation of ex...