A novel bead-based assay for selective measurement of arginase 1 activity in human serum [0.03%]
一种新型珠基测定人血清鸟胺酸脱羧酶活性的方法
Kenjiro Nagaoka,Noriyoshi Ogino,Keiki Ogino et al.
Kenjiro Nagaoka et al.
Arginase is widely used to analyze nitric oxide-related physiology and inflammation. However, traditional activity assays are often complicated and lack isoform resolution because of the shared substrates and products of arginase 1 (ARG1) a...
Integrated targeted and untargeted analysis of polar peptides in foods using hydrophilic interaction liquid chromatography-data-independent acquisition-mass spectrometry [0.03%]
基于亲水作用色谱-数据非依赖性采集质谱的食品极性肽类组学分析方法
Boudewijn Hollebrands,Germaine Thong,Hans-Gerd Janssen
Boudewijn Hollebrands
A hydrophilic interaction liquid chromatography-data-independent acquisition mass spectrometry (HILIC-DIA-MS) workflow was developed for simultaneous targeted semi-quantification of polar peptides and untargeted profiling of both peptides a...
Relevance analysis of N-glycan variations in C2C12 cells and mouse serum under simulated microgravity using a quaternary phosphonium hydrazide labeling strategy-based mass spectrometry quantitation approach [0.03%]
基于四元磷𬭩肼基标记策略质谱定量方法分析模拟微重力条件下C2C12细胞和小鼠血清N-糖链变异的相关性
Qian-Ru Ma,Jie-Cheng Lu,Yan-Qing Li et al.
Qian-Ru Ma et al.
Mass spectrometry combined with stable isotope labeling is a powerful technique for detecting disease-related changes in glycosylation patterns and identifying potential biomarkers. However, stable isotope labeling reagents that simultaneou...
Comparison of the phenolic and antioxidant potential of five European herbal remedies by effect-directed analysis using offline two-dimensional liquid chromatography-high resolution mass spectrometry [0.03%]
基于两维液相色谱-高分辨质谱的定向分析五种欧洲草药酚类和抗氧化潜力的比较研究
M Häßler,K Wetzel,T Tishakova et al.
M Häßler et al.
Herbal remedies contain various phenolic compounds. However, it remains difficult to identify the most important bioactive components and compare their effectiveness in different plant species. In this study, effect-directed analysis has be...
Opioid detection and quantification in plasma and oral fluid by LC-MS/MS [0.03%]
液相色谱-串联质谱法检测和定量血浆和口腔液体中的阿片类药物
Luana M Rosendo,Suzel Costa,Susana Simões et al.
Luana M Rosendo et al.
The opioid crisis remains a significant public health concern, necessitating the development of sensitive and reliable analytical methods for drug detection. This study aimed to develop and validate a liquid chromatography-tandem mass spect...
Neutralizing activity analysis of mimotopes against porcine epidemic diarrhea virus (PEDV) spike protein [0.03%]
猪流行性腹泻病毒刺突蛋白 Mimotope 中和活性分析
Tae-Hun Kim,Jae-Yeon Park,Soonil Kwon et al.
Tae-Hun Kim et al.
Mimotopes of the porcine epidemic diarrhea virus (PEDV) spike protein (SP) were screened from a Fv-antibody library, and the neutralizing activity of screened mimotopes was analyzed using plaque assay and docking simulation. The screened Fv...
Portable biosensor for quantitative detection of meat adulteration based on heparin sodium-mediated one-tube RPA/SCas12a amplification strategy [0.03%]
基于硫酸肝素钠介导的单管RPA/SCas12a扩增策略的便携式生物传感器用于肉制品掺假的定量检测
Guofeng Sun,Kai Shi
Guofeng Sun
Meat adulteration poses significant social concerns, including the infringement of consumer rights, potential risks of food allergies, and conflicts with religious dietary practices. Therefore, a pressing need exists for the creation of swi...
Quality assessment of Brazilian olive oils by GC×GC-MS and chemometrics [0.03%]
采用二维气相色谱-串联质谱和化学计量学评估巴西橄榄油的质量
Andre Cunha Paiva,Glaucimar Alex Passos de Resende,Luidy Darllan Barbosa et al.
Andre Cunha Paiva et al.
Virgin olive oil quality assessment is critical for consumer protection and industry regulation. Fraudulent activities involving virgin olive oil are common worldwide. In Brazil, recent nationwide inspections have revealed recurrent cases o...
Visualizing xenobiotics and metabolites in zebrafish by VUV-laser desorption post-ionization mass spectrometry imaging [0.03%]
利用VUV激光解吸后续离子模式质谱成像技术观测斑马鱼中的外源物及其代谢产物分布状况
Anan Li,Shijie Lu,Xueqing Shang et al.
Anan Li et al.
Mass spectrometry imaging (MSI) enables spatial mapping of chemical distributions across complex surfaces, with broad applications spanning materials analysis, life sciences, and clinical diagnostics. However, conventional ionization source...
Sensitive and selective detection of microRNA using hyperbranched rolling circle amplification based on symmetric dumbbell-type probe [0.03%]
基于对称哑铃型探针的分枝滚环反应放大技术检测microRNA及其应用研究
Shu-Ling Yan,Xiao-Tong Yang,Chun-Guang Yang et al.
Shu-Ling Yan et al.
Examining the changes in microRNA expression in vivo enhances the understanding of their tumor-associated functions and facilitates clinical assays based on their roles as disease markers. However, due to the low abundance and high homology...