Secretory Production of Heterologous Antimicrobial Peptides in Corynebacterium glutamicum [0.03%]
谷氨酸棒杆菌中异源抗菌肽的分泌表达
Wei Long,Lina Apitius,Patrick Lenz et al.
Wei Long et al.
Antimicrobial peptides (AMPs) are host defense peptides that act against a broad spectrum of microorganisms. AMPs are of high interest as medicinal products, antimicrobial coatings, and for controlling biofilm formation. Applications and re...
Real-Time VCC Monitoring and Forecasting in HEK-Cell-Based rAAV Vector Production Using Capacitance Spectroscopy [0.03%]
基于HEK细胞的rAAV载体生产过程中使用电容谱学进行实时VCC监测和预测
Rafael Machleid,Suneetha Nunna,Ajith George et al.
Rafael Machleid et al.
Recombinant adeno-associated virus (rAAV) vector production is a complex process in which the robust cultivation of human embryonic kidney cells (HEK293) plays a critical role in generating high-quality viral vectors. Tracking the viable ce...
Combination of Two-Stage Continuous Feeding and Optimized Synthetic Medium Increases Lipid Production in Lipomyces starkeyi [0.03%]
两步连续供料和优化合成培养基结合促进毛霉菌产脂
Chih-Chan Wu,Kenji Okano,Pijar Religia et al.
Chih-Chan Wu et al.
The oleaginous yeast Lipomyces starkeyi is recognized for its remarkable lipid accumulation under nitrogen-limited conditions. However, precise control of microbial lipid production in L. starkeyi remains challenging due to the complexity o...
Gesa Brauneck,Dominik Engel,Luca Antonia Grebe et al.
Gesa Brauneck et al.
For about 100 years, the shake flask has been established for biotechnological cultivations as one of the most important cultivation systems in early process development. Its appeal lies in its simple handling and highly versatile applicati...
Evaluation of Yeast Alcohol Acetyltransferases for Ethyl Acetate Production in Clostridium ljungdahlii [0.03%]
乙醇乙酸盐生产用酿酒酵母酒精酰基转移酶的筛选及在解糖弧菌中的表达与鉴定
Santiago T Boto,Kareem Gerges,Bettina Bardl et al.
Santiago T Boto et al.
Sustainable chemical production from C1 gaseous substrates, such as syngas or CO2/H2, can be achieved through gas fermentation. In gas fermentation, acetogenic bacteria are able to utilize oxidized inorganic carbon sources as the sole carbo...
Integrated SegFlow, µSIA, and UPLC for Online Sialic Acid Quantitation of Glycoproteins Directly from Bioreactors [0.03%]
整合SegFlow、µSIA和UPLC在线定量生物反应器中糖蛋白唾液酸含量
Letha Chemmalil,Tanmay Kulkarni,Mathura Raman et al.
Letha Chemmalil et al.
This study emphasizes the critical importance of closely monitoring and controlling the sialic acid content in therapeutic glycoproteins, including EPO, interferon-γ, Orencia, Enbrel, and others, as the level of sialylation directly impact...
Synthesis and Evaluation of PCL/Chitosan/CQD-Fe Magnetic Nanocomposite for Wound Healing: Emphasis on Gene Expression [0.03%]
聚己内酯/壳聚糖/CQD-Fe磁性纳米复合材料的合成与评价：促伤口愈合性能及基因表达研究
Elham Maghareh Abed,Fatemeh Yazdian,Abbas Akhavan Sepahi et al.
Elham Maghareh Abed et al.
The development of an effective and rapid method for healing the skin is of crucial importance. In this study, we prepared a porous scaffold made of polycaprolactone (PCL) and carbon quantum dots (CQDs), Fe, and Chitosan (Cs) as the scaffol...
Polyporus umbellatus, A Precious Rare Fungus With Good Pharmaceutical and Food Value [0.03%]
珍贵药食同源真菌雷丸功效的分子机理研究进展与展望
Sizhu Ren,Hua Liu,Qing Sang et al.
Sizhu Ren et al.
Polyporus umbellatus is a rare porous fungus that exhibits notable pharmacological activities. Particularly, due to its diuretic properties, it is considered an important source of targeted drugs for the treatment of kidney disease. Extensi...
A Consecutive Genome Engineering Method Reveals a New Phenotype and Regulation of Glucose and Glycerol Utilization in Clostridium Pasteurianum [0.03%]
连续基因组工程技术揭示了Clostridium pasteurianum中葡萄糖和甘油利用的新表型及调控机制
Tom Nguyen,Luca W G Meleski,Minu P Belavatta et al.
Tom Nguyen et al.
Clostridium pasteurianum is a microorganism for production of 1,3-propanediol (1,3-PDO) and butanol, but suffers from lacking genetic tools for metabolic engineering to improve product titers. Furthermore, previous studies of C. pasteurianu...
Succinic Acid Production With Actinobacillus succinogenes -Influence of an Electric Potential on the Intercellular NADH/NAD+ Balance [0.03%]
利用Actinobacillus succinogenes生产丁二酸—电位对细胞内NADH/NAD+平衡的影响
Jan-Niklas Hengsbach,Marcel Cwienczek,Wolfgang Laudensack et al.
Jan-Niklas Hengsbach et al.
Bioelectrochemical systems (BESs) offer a sustainable method for chemical production, including the enhanced production of succinic acid. By combining fermentation with BES, it could be possible to achieve sustainable succinic acid producti...